LOCUS       V043434                10343 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043434
VERSION     V043434
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4437
                     /label="Ncl(NM_010880)"
                     /note="Ncl(NM_010880)"
                     /gene="Ncl"
     promoter        4502..4713
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4726..4994
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5023..5032
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5053..5715
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5785..5838
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5839..6438
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6439..7026
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7100..7333
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7405..7526
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7545..7680
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7718..7734)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7742..7758
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7766..7796)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7811..7832
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8120..8708)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8879..9739)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9740..9844)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10318..10334
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg tgaagctcgc aaaggctggc
     2341 aaaacccacg gtgaggccaa gaaaatggct cctcctccaa aggaggtgga agaggatagt
     2401 gaagatgaag aaatgtcaga agatgaagat gacagcagtg gagaagagga ggttgtcatc
     2461 cctcagaaaa aaggcaaaaa ggctaccaca accccagcaa agaaggtggt tgtttcacaa
     2521 acaaaaaagg ctgcagttcc cacaccagct aagaaagcag ctgtgacccc aggcaaaaag
     2581 gcagtagcca caccagctaa gaaaaacatt acaccagcca aagtcattcc aacaccgggt
     2641 aagaagggag ctgcacaagc aaaagcgttg gtaccaactc ctggtaaaaa gggagctgcc
     2701 actccagcta agggggctaa gaacggtaag aatgccaaga aggaagacag tgatgaggat
     2761 gaagatgaag aggatgaaga tgatagcgat gaggatgaag atgatgagga agaggatgag
     2821 tttgagccac caatagtaaa aggagtgaag ccagcaaaag cagctcctgc tgctcctgcc
     2881 tcagaggatg aggaagatga tgaggatgaa gatgatgagg aagatgatga tgaagaggag
     2941 gaagatgact ctgaggaaga agttatggag atcacaacag ccaaaggaaa gaaaactcct
     3001 gcaaaagttg ttcctatgaa agccaagagt gtggctgagg aggaggatga tgaggaagag
     3061 gatgaagatg acgaggatga ggatgatgag gaagaggatg acgaagatga tgatgaggaa
     3121 gaagaggagg aagaacctgt taaagcagca cctggaaaac ggaagaagga gatgaccaag
     3181 cagaaagaag cccctgaagc caagaaacag aaagtagaag gctcagaacc aactacacct
     3241 ttcaatctgt tcattggaaa ccttaatcca aacaagtctg ttaatgaatt aaaatttgcc
     3301 atcagtgaac tttttgctaa aaatgatctt gctgttgtgg atgtcagaac tggtacaaat
     3361 aggaaatttg gttatgtgga ctttgagtct gctgaagacc tagaaaaggc cttggagctc
     3421 actggtttaa aagtgtttgg caatgaaatt aaactagaaa aaccaaaagg aagagatagt
     3481 aagaaagttc gagctgcaag aacacttcta gccaaaaacc tctctttcaa catcactgag
     3541 gatgaattaa aggaagtgtt tgaagacgcc atggagatca gattagtcag ccaggatggg
     3601 aaaagtaaag ggattgctta tattgaattt aagtctgaag ctgatgcaga gaaaaatttg
     3661 gaagaaaagc agggggcaga aattgatgga cgatctgttt cactctacta tactggagag
     3721 aaaggtcaaa ggcaagagag aactggaaag accagcactt ggagtggtga atcaaagact
     3781 ttggttttaa gtaacctttc ctacagtgca acaaaagaaa ctcttgagga agtatttgag
     3841 aaagcaactt ttatcaaagt gccccagaac ccacatggca aacctaaagg gtatgcattt
     3901 atagaatttg cttcatttga agatgctaaa gaagctttaa attcctgtaa taaaatggaa
     3961 attgagggca gaacaatcag gctggagttg caaggatcca attcgagaag tcaaccatcc
     4021 aaaactctgt ttgtcaaagg tctgtctgag gataccactg aagagacctt aaaagaatca
     4081 tttgagggct ctgttcgtgc aagaatagtc actgatcggg aaactggttc ttccaaaggg
     4141 tttggttttg tagactttaa tagtgaggaa gatgccaaag ctgccaagga ggccatggaa
     4201 gatggagaaa ttgacggaaa caaagttacc ttggactggg ccaaacctaa gggtgaaggt
     4261 ggctttggtg gtcgaggtgg aggcagagga ggtttcggag gcagaggtgg aggcagaggt
     4321 ggaagaggtg gatttggagg aagaggccgg ggaggctttg gaggtagagg aggcttccga
     4381 ggcggcagag gaggaggggg agacttcaag ccacaaggaa agaagacgaa gtttgaatag
     4441 ggatccctgc gatatccagc gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     4501 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     4561 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     4621 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     4681 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     4741 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     4801 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     4861 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     4921 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     4981 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     5041 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     5101 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     5161 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     5221 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     5281 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     5341 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     5401 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     5461 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     5521 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     5581 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     5641 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     5701 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     5761 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     5821 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     5881 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     5941 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6001 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6061 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6121 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6181 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6241 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6301 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6361 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6421 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6481 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6541 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6601 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6661 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6721 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6781 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     6841 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6901 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6961 cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct
     7021 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7081 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt
     7141 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7201 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7261 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7321 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     7381 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     7441 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7501 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     7561 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7621 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7681 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa
     7741 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7801 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7861 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7921 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7981 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8041 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8101 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8161 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8221 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8281 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8341 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8401 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8461 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8521 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     8581 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8641 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8701 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8761 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8821 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8881 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8941 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9001 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9061 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9121 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9181 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9241 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9301 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9361 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9421 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9481 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9541 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9601 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9661 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9721 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9781 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9841 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     9901 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     9961 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
    10021 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
    10081 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
    10141 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
    10201 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
    10261 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
    10321 aaacgacggc cagtgccaag ctg
//
