LOCUS       V027466                10938 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027466
VERSION     V027466
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..4957
                     /label="Nedd4(NM_010890)"
                     /note="Nedd4(NM_010890)"
                     /gene="Nedd4"
     CDS             4964..5053
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        5097..5308
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5321..5589
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5618..5627
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5648..6310
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6380..6433
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6434..7033
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7034..7621
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7695..7928
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8000..8121
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8140..8275
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8313..8329)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8337..8353
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8361..8391)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8406..8427
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8715..9303)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9474..10334)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10335..10439)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10913..10929
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatga gctcggacat ggcagccgac gagtcggagg ccccagtact
     2341 ctcggaggac gaggtatggg agttttgcct ggataagaca gaagatggtg gcggatcccc
     2401 cggaagtgat gttacagata cttgtgagcc tccatgtgga tgctgggagt tgaatccgaa
     2461 ttccctggaa gaggagcacg tgctgttcac tgctgatccg tacctggagc tccacaacga
     2521 tgacacacga gttgtgagag tgaaggttat agctggcata ggcctggcca agaaagacat
     2581 cttgggagcc agtgatcctt acgtaagagt gacattgtat gacccgatga gtggaatcct
     2641 taccagcgtg cagacaaaaa ctatcaaaaa gtctttgaat ccaaaatgga atgaagaaat
     2701 actgttcagg gtccttccac agcgacaccg cattcttttc gaagtgtttg atgaaaatcg
     2761 tttgacaaga gatgatttcc taggtcaagt ggatgtccct ctctatcctt taccgactga
     2821 aaacccaaga atggagagac catatacatt taaggatttt gttcttcatc caagaagtca
     2881 caaatcaaga gttaaaggtt atctgagatt aaaaatgact tatttaccta aaaatggctc
     2941 agaagatgaa aatgcagacc aggctgagga gttagagcct ggctgggttg ttttggacca
     3001 accagatgct gccactcatt tgccgcatcc accagaaccc tctcccctac ctccaggatg
     3061 ggaagagagg caggatgtcc ttggaaggac ctactacgta aaccatgaat ctagaagaac
     3121 acagtggaaa aggccaagcc ctgacgatga cctcacggat gaagacaatg atgatatgca
     3181 gctgcaagcg cagcgagcat tcaccaccag gcggcagata tcggaggatg tggatggccc
     3241 tgacaaccgg gagtcccctg agaattggga aatcgtacga gaagatgaaa acaccgagta
     3301 tagtggtcag gctgtccagt cacctccatc gggtcacatt gatgtgcaga ctcaccttgc
     3361 agaagagttt aataccagac ttgctgtgtg tggaaatcca gccaccagcc agccggttac
     3421 cagctcaaat cattccagca gaggaggcag cttgcagacc tgtatctttg aggaacagcc
     3481 tacacttcct gtgcttttgc ctacttcatc tggattgcca ccaggttggg aagaaaaaca
     3541 agatgacaga ggaagatcat actatgtaga ccacaactct aaaaccacca catggtccaa
     3601 gcccaccatg caggatgatc caagatcgaa aatccctgct catctgagag gaaagactga
     3661 ctccaatgac ctgggaccct tacctccagg ctgggaagaa agaacccaca cagatgggcg
     3721 agtcttcttc ataaaccaca atataaagaa gacccagtgg gaagatcctc gcctgcagaa
     3781 cgtggcaatc actggaccag cagtgcccta ctccagagat tacaagagaa agtacgagtt
     3841 cttcagaagg aagctcaaga agcagactga cattccaaac aaatttgaaa tgaagcttcg
     3901 ccgcgcaaac attctggagg attcttaccg gaggattatg ggtgtgaaga gagctgactt
     3961 gctcaaggcc agactctgga ttgagtttga tggtgaaaag ggccttgact atggaggggt
     4021 tgccagagag tggttcttcc tcatctcgaa ggaaatgttc aacccttact acggcctgtt
     4081 tgaatattct gctacggata attacaccct acagataaat cctaactcgg gcttgtgtaa
     4141 tgaagatcac ctctcatact tcaagttcat tggccgtgtg gctgggatgg cagtttatca
     4201 tggcaagctg ttggatgggt ttttcatccg tccgttttac aagatgatgc ttcagaaact
     4261 gataacactg cacgacatgg agtccgtgga tagtgaatat tacagttctc tgcgatggat
     4321 tcttgaaaat gacccgacgg agctggacct gagatttatc atagatgaag aactttttgg
     4381 acagacacat cagcacgaac tgaaaaccgg aggatcagag attgttgtca ccaataagaa
     4441 caaaaaggag tatatctacc ttgtaataca atggcgattt gtgaaccgta tccagaagca
     4501 aatggcagct tttaaagagg gattttttga actgatacca caggatctca tcaagatatt
     4561 tgatgaaaat gagctagagc ttctcatgtg tggtctggga gatgtggatg tgaacgactg
     4621 gcgggaacac acaaaataca aaaatggcta cagcatgaac caccaggtca tccactggtt
     4681 ctggaaggct gtttggatga tggattcgga aaaaagaata cgcttacttc agtttgtcac
     4741 tggcacatcc cgtgtgccga tgaatgggtt tgctgaactc tatggctcga atggaccaca
     4801 atccttcaca gtggaacaat ggggcacccc tgataagctg ccaagagcac acacctgctt
     4861 caatcgcctg gacctgccac cctacgaatc ctttgacgaa ctctgggata aacttcagat
     4921 ggcaattgag aacacacagg gctttgatgg cgttgatgga tccgaacaaa aactaatatc
     4981 agaggaagat ttggaacaga aactaatcag tgaagaagat ttagagcaaa agttaatttc
     5041 cgaggaggac ttatagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc
     5101 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
     5161 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     5221 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     5281 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
     5341 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     5401 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     5461 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     5521 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
     5581 ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag
     5641 cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt
     5701 cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa
     5761 gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg
     5821 cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa
     5881 caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt
     5941 gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac
     6001 cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt
     6061 ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag
     6121 cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc
     6181 catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga
     6241 gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat
     6301 cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc
     6361 cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga
     6421 gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     6481 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     6541 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     6601 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     6661 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     6721 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     6781 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     6841 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     6901 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     6961 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     7021 gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     7081 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     7141 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     7201 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     7261 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7321 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7381 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     7441 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7501 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7561 tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggc cgcctccccg
     7621 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7681 agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc
     7741 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7801 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7861 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7921 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7981 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     8041 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8101 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     8161 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8221 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
     8281 ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8341 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     8401 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     8461 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     8521 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     8581 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8641 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8701 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8761 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8821 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8881 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8941 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9001 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9061 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9121 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     9181 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9241 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9301 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9361 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9421 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9481 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9541 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9601 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9661 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9721 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9781 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9841 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9901 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9961 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10021 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10081 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10141 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10201 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10261 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10321 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10381 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10441 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
    10501 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
    10561 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
    10621 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
    10681 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
    10741 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
    10801 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
    10861 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
    10921 acggccagtg ccaagctg
//
