LOCUS       V029249                11149 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029249
VERSION     V029249
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5175
                     /label="Nod1(NM_172729)"
                     /note="Nod1(NM_172729)"
                     /gene="Nod1"
     CDS             5182..5247
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5291..5502
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5515..5783
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5812..5821
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5842..6504
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6574..6627
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6628..7227
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7228..7816
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7890..8123
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8195..8316
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8356..8491
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8524..8540)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8548..8564
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8572..8602)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8617..8638
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8926..9514)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9685..10545)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10546..10650)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11124..11140
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg aggaacacgg ccatcatgag
     2341 atggaaggca ccccattggg ttgtcactcc cacattaaac tgctgaagat caacagggaa
     2401 catctggtca ccaacattcg gaacactcag tgtctggtgg acaacttgct ggagaatggc
     2461 tacttctcag ccgaagatgc agagattgtg tgtgcctgtc ccaccaagcc tgacaaggtc
     2521 cgaaagatcc ttgacctggt gcagagcaaa ggcgaggagg tgtctgagtt cttcctctac
     2581 gtgctgcagc agctggagga tgcttacgtg gacctcaggc tgtggctctc agaaattggc
     2641 ttctcccctt cccagctcat tcggaccaaa actatcgtca atactgaccc agtaagcagg
     2701 tatacccaac agctgcgaca ccaactgggc cgcgactcca agttcatgct gtgctacgcc
     2761 cagaaggagg acctgctgct ggaggagacc tatatggaca cactcatgga gctggtaggc
     2821 ttcaacaatg aaaacctggg cagcctagga ggcctggatt gcctgctgga ccacagtacg
     2881 ggcgtcctca acgagcatgg cgagactgtc ttcgtgttcg gggacgcggg agtgggcaag
     2941 tccatgctgc tgcagaggtt gcagagcctc tgggcgtcag gcaggttgac ctccacagcc
     3001 aaattcttct tccacttccg ctgccgcatg ttcagctgct tcaaggagag cgacatgctg
     3061 agtctgcagg acctgctctt caagcatttc tgctacccgg agcaggaccc cgaggaggtg
     3121 ttctccttct tgctgcgctt tccccacaca gcgctcttca cttttgacgg cctggatgag
     3181 ctgcactcag acttcgacct gagccgcgtg ccggatagct gctgcccctg ggagccggct
     3241 caccctctgg tcctgctggc taacctccta agtgggaggc tgctcaaggg tgccggcaaa
     3301 ttgctcactg ctcgcacagg cgtggaggtc ccccgccagc tcctgcgcaa aaaggtgctg
     3361 ctccggggct tctccccaag tcacctgcgc gcctatgccc gccggatgtt ccccgagcgc
     3421 acagcgcagg agcatctgct gcagcagctg gatgccaacc ccaacctctg cagcctgtgc
     3481 ggggtgccgc tcttctgttg gatcatcttc cgttgtttcc agcacttcca gacggtcttc
     3541 gagggctcct cttcacagtt gccggactgt gctgtgaccc tgaccgatgt ctttctgctg
     3601 gtcactgagg tgcatctgaa caggccgcag cccagcagcc tggtgcagcg caacacgcgc
     3661 agcccggcgg aaaccctacg tgcaggctgg cgcacgctgc atgcgctggg agaggtggct
     3721 caccgaggca ccgacaagag cctctttgtg tttggccagg aggaggtgca ggcgtcgaag
     3781 ctgcaggaag gagatctgca gctgggcttc ctgcgggctt tgcccgatgt gggccctgag
     3841 cagggccagt cttacgaatt tttccacctt acgctccagg ccttcttcac cgccttcttc
     3901 ctggtagcag atgacaaagt gagcacccgg gagttgctga ggttctttcg agaatggacg
     3961 tctcctggag aggcaacaag ctcgtcctgc cattcttcct tcttctcctt ccagtgcctg
     4021 ggcggcagaa gccggttggg ccctgatcct ttcaggaaca aagatcactt ccagttcacc
     4081 aacctcttcc tgtgcgggct actggccaaa gcccgacaga aactccttcg gcagctggtg
     4141 cccaaggcta tcctgaggag gaagcgcaag gccctgtggg ctcacctgtt tgctagcctg
     4201 cgctcctact tgaagagcct acctcgggtc cagtctggag gctttaacca ggtgcatgcc
     4261 atgcccacat tcctgtggat gctgcgctgc atctatgaga cgcagagcca gaaggtgggg
     4321 cgcctcgccg ccaggggcat cagtgcggac tacctcaagc tggccttttg caacgcttgc
     4381 tctgcggact gcagcgccct gtccttcgtc ctgcatcact tccacaggca gctggcccta
     4441 gacctggaca acaacaacct caatgactat ggcgtgcagg agctgcagcc ttgctttagc
     4501 cgtctcacgg ttatcagact cagcgtcaac cagatcaccg acacgggggt gaaggtgcta
     4561 tgtgaggaac tgaccaagta taagatcgtg acgttcctgg gtttatacaa caaccagata
     4621 actgatatcg gagccaggta tgtggcccaa atcctggatg aatgcagagg cctcaagcac
     4681 cttaaactag ggaaaaacag aataacaagt gagggcggga agtgtgtggc tttggctgtg
     4741 aagaacagca cctccatcgt tgatgttggg atgtggggta atcagattgg agacgaaggg
     4801 gcaaaggcct tcgcagaggc attgaaggac caccccagcc tgaccactct cagtcttgca
     4861 ttcaatggca tctctccgga gggagggaag agccttgcgc aggccctgaa gcagaacacc
     4921 acactgacag taatctggct gaccaaaaat gaacttaatg atgagtctgc agagtgcttc
     4981 gctgagatgc tgagagtgaa ccagacgcta cggcatttat ggctgatcca gaatcgcatc
     5041 acagccaagg ggacagcgca gctggcgagg gcactgcaga agaacacagc cataacagag
     5101 atttgtctca atggaaactt gattaagccc gaggaggcca aagtcttcga gaatgagaag
     5161 agaatcatct gcttcggatc cgactacaaa gaccatgacg gtgattataa agatcatgac
     5221 atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga tcgctccggt
     5281 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     5341 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     5401 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     5461 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     5521 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     5581 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     5641 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     5701 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     5761 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg
     5821 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg
     5881 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg
     5941 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac
     6001 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc
     6061 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc
     6121 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc
     6181 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc
     6241 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc
     6301 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg
     6361 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc
     6421 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc
     6481 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac
     6541 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc
     6601 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc
     6661 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     6721 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     6781 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     6841 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     6901 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     6961 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     7021 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     7081 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     7141 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     7201 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga
     7261 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7321 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7381 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7441 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7501 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7561 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7621 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7681 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7741 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7801 tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct
     7861 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata
     7921 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7981 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8041 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8101 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     8161 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     8221 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8281 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     8341 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8401 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     8461 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat
     8521 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     8581 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8641 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8701 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8761 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8821 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8881 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8941 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9001 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9061 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9121 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9181 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9241 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9301 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9361 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9421 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9481 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9541 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9601 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9661 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9721 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9781 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9841 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9901 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9961 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    10021 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    10081 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    10141 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    10201 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    10261 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10321 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10381 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10441 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10501 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10561 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10621 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10681 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    10741 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
    10801 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    10861 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
    10921 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    10981 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    11041 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
    11101 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
