LOCUS V038879 8047 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038879 VERSION V038879 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..2901 /label="Nt5c(NM_015807)" /note="Nt5c(NM_015807)" /gene="Nt5c" CDS 2908..2973 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 3017..3228 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3241..3509 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 3536..4135 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 4142..4730 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 4804..5037 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 5109..5230 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 5249..5384 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(5422..5438) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5446..5462 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5470..5500) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5515..5536 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5824..6412) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6583..7443) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7444..7548) /gene="bla" /label="AmpR promoter" primer_bind 8022..8038 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggcggtg aagcggcccg tgcgcgtgct ggtggacatg 2341 gacggcgtgc tagctgattt cgagtccggc ctcctgcagg gttttcgccg ccgctttccc 2401 gaggaaccgc acgtgccgct ggaacagcgc cggggcttcc tcgccaacga gcagtacgga 2461 gctctgaggc cggacctggc ggaaaaagtg gccagtgtgt atgaatctcc aggctttttc 2521 ctaaacttgg agcccatccc tggggccttg gacgctttgc gagagatgaa cgacatgaaa 2581 gacaccgagg tcttcatctg caccacccca ctgctgaagt acgaccactg tgtgggcgag 2641 aagtaccgct gggtggagca gaacctgggg ccagagtttg tggagcggat tatcttgact 2701 agggacaaga cggtggtcat gggagacctg ctcattgatg acaaagacaa cattcaaggc 2761 ctagaggaga ccccaagctg ggagcacatc ttgttcacct gctgccacaa tcagcacctg 2821 gccctgcccc ccaccaggag acggctgctt tcctggagtg acaactggag ggggatcatt 2881 gagagcaagc gagccagcct gggatccgac tacaaagacc atgacggtga ttataaagat 2941 catgacatcg actacaagga tgacgatgac aagtagcggc cgcgaaggat ctgcgatcgc 3001 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 3061 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 3121 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 3181 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 3241 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 3301 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 3361 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 3421 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 3481 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagatgac 3541 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac 3601 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca 3661 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg 3721 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt 3781 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg 3841 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc 3901 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc 3961 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac 4021 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt 4081 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagtcga 4141 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 4201 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 4261 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 4321 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 4381 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 4441 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 4501 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct 4561 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 4621 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 4681 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt 4741 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 4801 gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt 4861 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 4921 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 4981 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 5041 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 5101 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 5161 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 5221 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca 5281 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 5341 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga 5401 cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 5461 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 5521 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 5581 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 5641 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 5701 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 5761 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 5821 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 5881 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 5941 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 6001 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 6061 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 6121 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 6181 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 6241 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 6301 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 6361 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 6421 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 6481 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 6541 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 6601 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 6661 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 6721 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 6781 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 6841 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 6901 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 6961 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 7021 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 7081 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 7141 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 7201 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 7261 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 7321 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 7381 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 7441 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 7501 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 7561 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 7621 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 7681 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 7741 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 7801 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 7861 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 7921 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 7981 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 8041 caagctg //