LOCUS       V036238                10819 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036238
VERSION     V036238
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4845
                     /label="OTUD7B(NM_020205)"
                     /note="OTUD7B(NM_020205)"
                     /gene="OTUD7B"
     CDS             4852..4917
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4961..5172
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5185..5453
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5482..5491
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5512..6174
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6244..6297
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6298..6897
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6898..7486
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7560..7793
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7865..7986
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8026..8161
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8194..8210)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8218..8234
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8242..8272)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8287..8308
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8596..9184)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9355..10215)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10216..10320)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10794..10810
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga ccctggacat ggatgctgtt
     2341 ctgtcagatt ttgtccgttc cacaggagca gagccagggc tagcgcgaga tctcctagaa
     2401 ggaaagaatt gggatgtgaa tgccgccctc agtgattttg aacagctacg tcaagtccat
     2461 gctggaaacc tacccccatc ctttagtgag gggagtggtg gctccaggac ccctgaaaaa
     2521 gggttttctg acagagagcc tactcgccct ccccgaccca tcctccagcg gcaggatgac
     2581 atcgttcaag aaaaacgcct gtctaggggc atctcccacg ccagctccag cattgtttcc
     2641 ctggcccggt cccatgtctc ctccaatggt gggggtgggg ggagcaatga gcaccccctg
     2701 gaaatgccca tctgtgcctt ccagcttcca gatctcactg tatacaatga agacttccgc
     2761 agcttcatag agagagacct cattgagcag tccatgctgg ttgccttgga acaggcaggg
     2821 cgtttgaact ggtgggtgag tgtggatccc acctctcaga ggctgcttcc tttggcaact
     2881 actggagatg ggaactgcct cctgcatgca gcctcccttg gaatgtgggg tttccatgat
     2941 cgggacttga tgctgcggaa agctttgtat gcactgatgg agaagggagt tgagaaggaa
     3001 gcgttgaaaa ggcgctggag gtggcagcag acacagcaga ataaagagtc agggctggta
     3061 tacacagaag atgaatggca gaaggagtgg aatgaactga tcaagcttgc ctcaagtgaa
     3121 ccccgaatgc atctaggtac caatggagcc aactgtggtg gggtggagag ttctgaggag
     3181 cctgtatatg agagccttga agagtttcac gtctttgtcc ttgctcatgt gcttaggagg
     3241 cccatagtcg tcgtggcaga caccatgctg agggactccg gaggggaagc atttgcccct
     3301 attccctttg gaggaatcta tctgcctttg gaggtcccag ccagccagtg tcaccgctcc
     3361 cctctggtgc tcgcctatga tcaggcccac ttttctgcac tcgtgtccat ggagcagaag
     3421 gagaatacca aggaacaagc tgtgatccca cttacagatt cagagtataa gctgctgccc
     3481 ttgcactttg ctgtggaccc tggaaagggc tgggagtggg gcaaagatga tagtgacaat
     3541 gtccgattgg ccagtgtaat tctgtcccta gaggtcaaat tgcatctgct gcatagctac
     3601 atgaatgtga agtggatccc actgtcctct gatgcacagg ctcctctggc ccagcctgag
     3661 tcccccaccg cctcagctgg agatgagccc cggtccactc ctgagtctgg agactcagac
     3721 aaggagtcag ttggcagcag ttccaccagc aacgagggcg gccggcggaa ggagaagtca
     3781 aagcgagatc gggagaagga caagaagaga gcagattctg tggctaacaa actgggcagc
     3841 tttggcaaaa ccttgggcag caagctcaag aagaacatgg ggggcctgat gcacagcaag
     3901 ggttcaaagc ctggaggggt ggggacaggg ttgggaggaa gcagcggcac tgagacactg
     3961 gagaagaaga agaaaaactc actgaagagc tggaagggtg gcaaggagga ggcagctggg
     4021 gatgggcctg tgtctgagaa gcccccagct gagtctgttg gtaacggagg gagcaagtat
     4081 agccaggagg tgatgcagag cctgagcatt ctgaggactg ccatgcaagg ggaggggaag
     4141 tttatttttg ttggaaccct gaagatgggt caccgtcacc agtatcagga ggaaatgatc
     4201 cagcgctacc tttctgatgc tgaggagaga ttcctggcag aacagaagca gaaggaggca
     4261 gagaggaaga tcatgaatgg aggaataggg ggtggccctc ctccagccaa aaagccagag
     4321 ccagatgcta gggaagagca gccgaccggt cccccagcag agtccagggc aatggcattt
     4381 tccactggct accctgggga ctttactatc cctcggccgt ctgggggcgg agtccactgc
     4441 caggaacccc ggaggcagtt ggcagggggt ccatgtgtcg ggggcctacc accatatgcc
     4501 accttcccca gacagtgccc tcctgggcga ccctaccccc accaggacag catcccttct
     4561 ctggagccag gcagccactc taaggatgga cttcacaggg gtgccttgtt accacccccc
     4621 taccgagtgg ctgattccta tagcaatggc tacagagagc cccctgagcc agatggatgg
     4681 gctggaggtc tccggggcct tcccccaact cagaccaaat gcaaacaacc gaactgcagc
     4741 ttctatggac accctgagac aaacaacttc tgttcctgtt gttacaggga agaactgagg
     4801 aggagggagc gggaaccgga tggggagctc ctggtgcaca ggttcggatc cgactacaaa
     4861 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtag
     4921 cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     4981 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     5041 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     5101 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     5161 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     5221 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     5281 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     5341 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     5401 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     5461 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg
     5521 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc
     5581 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc
     5641 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc
     5701 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc
     5761 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc
     5821 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac
     5881 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc
     5941 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc
     6001 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc
     6061 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc
     6121 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc
     6181 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct
     6241 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg
     6301 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     6361 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     6421 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     6481 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     6541 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     6601 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     6661 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     6721 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     6781 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     6841 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat
     6901 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     6961 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     7021 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     7081 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     7141 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     7201 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7261 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     7321 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7381 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     7441 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag
     7501 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     7561 ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta ctgggtctct
     7621 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7681 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7741 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     7801 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     7861 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7921 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7981 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     8041 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8101 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8161 ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
     8221 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8281 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8341 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8401 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8461 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8521 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8581 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8641 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8701 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8761 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8821 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8881 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8941 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9001 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     9061 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9121 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9181 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9241 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9301 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9361 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9421 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9481 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9541 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9601 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9661 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9721 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9781 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9841 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9901 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9961 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10021 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10081 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10141 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10201 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10261 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10321 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    10381 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    10441 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    10501 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    10561 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    10621 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    10681 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    10741 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    10801 gacggccagt gccaagctg
//
