LOCUS       V034519                10447 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034519
VERSION     V034519
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4473
                     /label="PAK5(NM_177990)"
                     /note="PAK5(NM_177990)"
                     /gene="PAK5"
     CDS             4480..4545
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4589..4800
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4813..5081
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5110..5119
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5140..5802
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5872..5925
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5926..6525
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6526..7114
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7188..7421
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7493..7614
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7654..7789
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7822..7838)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7846..7862
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7870..7900)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7915..7936
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8224..8812)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8983..9843)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9844..9948)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10422..10438
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt ttgggaagaa aaagaaaaag
     2341 attgaaatat ctggcccgtc caactttgaa cacagggttc atactgggtt tgatccacaa
     2401 gagcagaagt ttaccggcct tccccagcag tggcacagcc tgttagcaga tacggccaac
     2461 aggccaaagc ctatggtgga cccttcatgc atcacaccca tccagctggc tcctatgaag
     2521 acaatcgtta gaggaaacaa accctgcaag gaaacctcca tcaacggcct gctagaggat
     2581 tttgacaaca tctcggtgac tcgctccaac tccctaagga aagaaagccc acccacccca
     2641 gatcagggag cctccagcca cggtccaggc cacgcggaag aaaatggctt catcaccttc
     2701 tcccagtatt ccagcgaatc cgatactact gctgactaca cgaccgaaaa gtacagggag
     2761 aagagtctct atggagatga tctggatccg tattatagag gcagccacgc agccaagcaa
     2821 aatgggcacg taatgaaaat gaagcacggg gaggcctact attctgaggt gaagcctttg
     2881 aaatccgatt ttgccagatt ttctgccgat tatcactcac atttggactc actgagcaaa
     2941 ccaagtgaat acagtgacct caagtgggag tatcagagag cctcgagtag ctcccctctg
     3001 gattattcat tccaattcac accttctaga actgcaggga ccagcgggtg ctccaaggag
     3061 agcctggcgt acagtgaaag tgaatgggga cccagcctgg atgactatga caggaggcca
     3121 aagtcttcgt acctgaatca gacaagccct cagcccacca tgcggcagag gtccaggtca
     3181 ggctcgggac tccaggaacc gatgatgcca tttggagcaa gtgcatttaa aacccatccc
     3241 caaggacact cctacaactc ctacacctac cctcgcttgt ccgagcccac aatgtgcatt
     3301 ccaaaggtgg attacgatcg agcacagatg gtcctcagcc ctccactgtc agggtctgac
     3361 acctacccca ggggccctgc caaactacct caaagtcaaa gcaaatcggg ctattcctca
     3421 agcagtcacc agtacccgtc tgggtaccac aaagccacct tgtaccatca cccctccctg
     3481 cagagcagtt cgcagtacat ctccacggct tcctacctga gctccctcag cctctcatcc
     3541 agcacctacc cgccgcccag ctggggctcc tcctccgacc agcagccctc cagggtgtcc
     3601 catgaacagt ttcgggcggc cctgcagctg gtggtcagcc caggagaccc cagggaatac
     3661 ttggccaact ttatcaaaat cggggaaggc tcaaccggca tcgtatgcat cgccaccgag
     3721 aaacacacag ggaaacaagt tgcagtgaag aaaatggacc tccggaagca acagagacga
     3781 gaactgcttt tcaatgaggt cgtgatcatg cgggattacc accatgacaa tgtggttgac
     3841 atgtacagca gctaccttgt cggcgatgag ctctgggtgg tcatggagtt tctagaaggt
     3901 ggtgccttga cagacattgt gactcacacc agaatgaatg aagaacagat agctactgtc
     3961 tgcctgtcag ttctgagagc tctctcctac cttcataacc aaggagtgat tcacagggac
     4021 ataaaaagtg actccatcct cctgacaagc gatggccgga taaagttgtc tgattttggt
     4081 ttctgtgctc aagtttccaa agaggtgccg aagaggaaat cattggttgg cactccctac
     4141 tggatggccc ctgaggtgat ttctaggcta ccttatggga cagaggtgga catctggtcc
     4201 ctcgggatca tggtgataga aatgattgat ggcgagcccc cctacttcaa tgagcctccc
     4261 ctccaggcga tgcggaggat ccgggacagt ttacctccaa gagtgaagga cctacacaag
     4321 gtttcttcag tgctccgggg attcctagac ttgatgttgg tgagggagcc ctctcagaga
     4381 gcaacagccc aggaactcct cggacatcca ttcttaaaac tagcaggtcc accgtcttgc
     4441 atcgtccccc tcatgagaca atacaggcat cacggatccg actacaaaga ccatgacggt
     4501 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     4561 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     4621 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     4681 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     4741 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     4801 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     4861 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     4921 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     4981 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     5041 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     5101 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     5161 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     5221 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     5281 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     5341 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     5401 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     5461 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     5521 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     5581 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     5641 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     5701 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     5761 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     5821 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     5881 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     5941 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6001 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6061 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6121 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6181 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6241 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6301 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6361 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6421 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6481 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     6541 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6601 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6661 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6721 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     6781 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     6841 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     6901 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     6961 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7021 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7081 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
     7141 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     7201 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca
     7261 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7321 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7381 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     7441 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     7501 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7561 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7621 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7681 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7741 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     7801 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7861 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7921 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7981 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8041 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8101 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8161 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8221 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8281 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8341 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8401 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8461 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8521 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8581 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8641 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     8701 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8761 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8821 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8881 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8941 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9001 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9061 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9121 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9181 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9241 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9301 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9361 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9421 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9481 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9541 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9601 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9661 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9721 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9781 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9841 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9901 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9961 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    10021 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    10081 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    10141 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
    10201 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    10261 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
    10321 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    10381 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    10441 caagctg
//
