LOCUS       V030612                10513 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030612
VERSION     V030612
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5343
                     /label="PARP1(NM_001618)"
                     /note="PARP1(NM_001618)"
                     /gene="PARP1"
     CDS             5350..5439
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        5483..5694
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5707..5975
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6002..6601
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6608..7196
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7270..7503
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7575..7696
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7715..7850
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7888..7904)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7912..7928
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7936..7966)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7981..8002
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8290..8878)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9049..9909)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9910..10014)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10488..10504
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggcggag tcttcggata agctctatcg agtcgagtac
     2341 gccaagagcg ggcgcgcctc ttgcaagaaa tgcagcgaga gcatccccaa ggactcgctc
     2401 cggatggcca tcatggtgca gtcgcccatg tttgatggaa aagtcccaca ctggtaccac
     2461 ttctcctgct tctggaaggt gggccactcc atccggcacc ctgacgttga ggtggatggg
     2521 ttctctgagc ttcggtggga tgaccagcag aaagtcaaga agacagcgga agctggagga
     2581 gtgacaggca aaggccagga tggaattggt agcaaggcag agaagactct gggtgacttt
     2641 gcagcagagt atgccaagtc caacagaagt acgtgcaagg ggtgtatgga gaagatagaa
     2701 aagggccagg tgcgcctgtc caagaagatg gtggacccgg agaagccaca gctaggcatg
     2761 attgaccgct ggtaccatcc aggctgcttt gtcaagaaca gggaggagct gggtttccgg
     2821 cccgagtaca gtgcgagtca gctcaagggc ttcagcctcc ttgctacaga ggataaagaa
     2881 gccctgaaga agcagctccc aggagtcaag agtgaaggaa agagaaaagg cgatgaggtg
     2941 gatggagtgg atgaagtggc gaagaagaaa tctaaaaaag aaaaagacaa ggatagtaag
     3001 cttgaaaaag ccctaaaggc tcagaacgac ctgatctgga acatcaagga cgagctaaag
     3061 aaagtgtgtt caactaatga cctgaaggag ctactcatct tcaacaagca gcaagtgcct
     3121 tctggggagt cggcgatctt ggaccgagta gctgatggca tggtgttcgg tgccctcctt
     3181 ccctgcgagg aatgctcggg tcagctggtc ttcaagagcg atgcctatta ctgcactggg
     3241 gacgtcactg cctggaccaa gtgtatggtc aagacacaga cacccaaccg gaaggagtgg
     3301 gtaaccccaa aggaattccg agaaatctct tacctcaaga aattgaaggt taaaaaacag
     3361 gaccgtatat tccccccaga aaccagcgcc tccgtggcgg ccacgcctcc gccctccaca
     3421 gcctcggctc ctgctgctgt gaactcctct gcttcagcag ataagccatt atccaacatg
     3481 aagatcctga ctctcgggaa gctgtcccgg aacaaggatg aagtgaaggc catgattgag
     3541 aaactcgggg ggaagttgac ggggacggcc aacaaggctt ccctgtgcat cagcaccaaa
     3601 aaggaggtgg aaaagatgaa taagaagatg gaggaagtaa aggaagccaa catccgagtt
     3661 gtgtctgagg acttcctcca ggacgtctcc gcctccacca agagccttca ggagttgttc
     3721 ttagcgcaca tcttgtcccc ttggggggca gaggtgaagg cagagcctgt tgaagttgtg
     3781 gccccaagag ggaagtcagg ggctgcgctc tccaaaaaaa gcaagggcca ggtcaaggag
     3841 gaaggtatca acaaatctga aaagagaatg aaattaactc ttaaaggagg agcagctgtg
     3901 gatcctgatt ctggactgga acactctgcg catgtcctgg agaaaggtgg gaaggtcttc
     3961 agtgccaccc ttggcctggt ggacatcgtt aaaggaacca actcctacta caagctgcag
     4021 cttctggagg acgacaagga aaacaggtat tggatattca ggtcctgggg ccgtgtgggt
     4081 acggtgatcg gtagcaacaa actggaacag atgccgtcca aggaggatgc cattgagcac
     4141 ttcatgaaat tatatgaaga aaaaaccggg aacgcttggc actccaaaaa tttcacgaag
     4201 tatcccaaaa agttctaccc cctggagatt gactatggcc aggatgaaga ggcagtgaag
     4261 aagctgacag taaatcctgg caccaagtcc aagctcccca agccagttca ggacctcatc
     4321 aagatgatct ttgatgtgga aagtatgaag aaagccatgg tggagtatga gatcgacctt
     4381 cagaagatgc ccttggggaa gctgagcaaa aggcagatcc aggccgcata ctccatcctc
     4441 agtgaggtcc agcaggcggt gtctcagggc agcagcgact ctcagatcct ggatctctca
     4501 aatcgctttt acaccctgat cccccacgac tttgggatga agaagcctcc gctcctgaac
     4561 aatgcagaca gtgtgcaggc caaggtggaa atgcttgaca acctgctgga catcgaggtg
     4621 gcctacagtc tgctcagggg agggtctgat gatagcagca aggatcccat cgatgtcaac
     4681 tatgagaagc tcaaaactga cattaaggtg gttgacagag attctgaaga agccgagatc
     4741 atcaggaagt atgttaagaa cactcatgca accacacaca atgcgtatga cttggaagtc
     4801 atcgatatct ttaagataga gcgtgaaggc gaatgccagc gttacaagcc ctttaagcag
     4861 cttcataacc gaagattgct gtggcacggg tccaggacca ccaactttgc tgggatcctg
     4921 tcccagggtc ttcggatagc cccgcctgaa gcgcccgtga caggctacat gtttggtaaa
     4981 gggatctatt tcgctgacat ggtctccaag agtgccaact actgccatac gtctcaggga
     5041 gacccaatag gcttaatcct gttgggagaa gttgcccttg gaaacatgta tgaactgaag
     5101 cacgcttcac atatcagcaa gttacccaag ggcaagcaca gtgtcaaagg tttgggcaaa
     5161 actacccctg atccttcagc taacattagt ctggatggtg tagacgttcc tcttgggacc
     5221 gggatttcat ctggtgtgaa tgacacctct ctactatata acgagtacat tgtctatgat
     5281 attgctcagg taaatctgaa gtatctgctg aaactgaaat tcaattttaa gacctccctg
     5341 tggggatccg aacaaaaact aatatcagag gaagatttgg aacagaaact aatcagtgaa
     5401 gaagatttag agcaaaagtt aatttccgag gaggacttat agcggccgcg aaggatctgc
     5461 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     5521 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     5581 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     5641 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     5701 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     5761 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     5821 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     5881 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     5941 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta
     6001 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt
     6061 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga
     6121 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     6181 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     6241 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     6301 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     6361 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     6421 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     6481 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     6541 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     6601 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6661 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6721 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6781 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6841 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6901 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6961 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     7021 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7081 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7141 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     7201 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7261 aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta
     7321 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7381 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7441 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7501 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7561 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7621 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7681 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc
     7741 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7801 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg
     7861 cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7921 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7981 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8041 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8101 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8161 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8221 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8281 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8341 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8401 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8461 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8521 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8581 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8641 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8701 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     8761 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8821 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8881 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8941 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9001 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9061 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9121 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9181 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9241 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9301 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9361 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9421 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9481 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9541 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9601 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9661 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9721 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9781 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9841 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9901 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9961 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10021 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    10081 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    10141 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    10201 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    10261 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    10321 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    10381 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    10441 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    10501 cagtgccaag ctg
//
