LOCUS       V044483                10462 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044483
VERSION     V044483
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4488
                     /label="PIK3R1(NM_181523)"
                     /note="PIK3R1(NM_181523)"
                     /gene="PIK3R1"
     CDS             4495..4560
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4604..4815
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4828..5096
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5125..5134
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5155..5817
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5887..5940
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5941..6540
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6541..7129
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7203..7436
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7508..7629
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7669..7804
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7837..7853)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7861..7877
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7885..7915)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7930..7951
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8239..8827)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8998..9858)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9859..9963)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10437..10453
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga gtgctgaggg gtaccagtac
     2341 agagcgctgt atgattataa aaaggaaaga gaagaagata ttgacttgca cttgggtgac
     2401 atattgactg tgaataaagg gtccttagta gctcttggat tcagtgatgg acaggaagcc
     2461 aggcctgaag aaattggctg gttaaatggc tataatgaaa ccacagggga aaggggggac
     2521 tttccgggaa cttacgtaga atatattgga aggaaaaaaa tctcgcctcc cacaccaaag
     2581 ccccggccac ctcggcctct tcctgttgca ccaggttctt cgaaaactga agcagatgtt
     2641 gaacaacaag ctttgactct cccggatctt gcagagcagt ttgcccctcc tgacattgcc
     2701 ccgcctcttc ttatcaagct cgtggaagcc attgaaaaga aaggtctgga atgttcaact
     2761 ctatacagaa cacagagctc cagcaacctg gcagaattac gacagcttct tgattgtgat
     2821 acaccctccg tggacttgga aatgatcgat gtgcacgttt tggctgacgc tttcaaacgc
     2881 tatctcctgg acttaccaaa tcctgtcatt ccagcagccg tttacagtga aatgatttct
     2941 ttagctccag aagtacaaag ctccgaagaa tatattcagc tattgaagaa gcttattagg
     3001 tcgcctagca tacctcatca gtattggctt acgcttcagt atttgttaaa acatttcttc
     3061 aagctctctc aaacctccag caaaaatctg ttgaatgcaa gagtactctc tgaaattttc
     3121 agccctatgc ttttcagatt ctcagcagcc agctctgata atactgaaaa cctcataaaa
     3181 gttatagaaa ttttaatctc aactgaatgg aatgaacgac agcctgcacc agcactgcct
     3241 cctaaaccac caaaacctac tactgtagcc aacaacggta tgaataacaa tatgtcctta
     3301 caagatgctg aatggtactg gggagatatc tcgagggaag aagtgaatga aaaacttcga
     3361 gatacagcag acgggacctt tttggtacga gatgcgtcta ctaaaatgca tggtgattat
     3421 actcttacac taaggaaagg gggaaataac aaattaatca aaatatttca tcgagatggg
     3481 aaatatggct tctctgaccc attaaccttc agttctgtgg ttgaattaat aaaccactac
     3541 cggaatgaat ctctagctca gtataatccc aaattggatg tgaaattact ttatccagta
     3601 tccaaatacc aacaggatca agttgtcaaa gaagataata ttgaagctgt agggaaaaaa
     3661 ttacatgaat ataacactca gtttcaagaa aaaagtcgag aatatgatag attatatgaa
     3721 gaatataccc gcacatccca ggaaatccaa atgaaaagga cagctattga agcatttaat
     3781 gaaaccataa aaatatttga agaacagtgc cagacccaag agcggtacag caaagaatac
     3841 atagaaaagt ttaaacgtga aggcaatgag aaagaaatac aaaggattat gcataattat
     3901 gataagttga agtctcgaat cagtgaaatt attgacagta gaagaagatt ggaagaagac
     3961 ttgaagaagc aggcagctga gtatcgagaa attgacaaac gtatgaacag cattaaacca
     4021 gaccttatcc agctgagaaa gacgagagac caatacttga tgtggttgac tcaaaaaggt
     4081 gttcggcaaa agaagttgaa cgagtggttg ggcaatgaaa acactgaaga ccaatattca
     4141 ctggtggaag atgatgaaga tttgccccat catgatgaga agacatggaa tgttggaagc
     4201 agcaaccgaa acaaagctga aaacctgttg cgagggaagc gagatggcac ttttcttgtc
     4261 cgggagagca gtaaacaggg ctgctatgcc tgctctgtag tggtggacgg cgaagtaaag
     4321 cattgtgtca taaacaaaac agcaactggc tatggctttg ccgagcccta taacttgtac
     4381 agctctctga aagaactggt gctacattac caacacacct cccttgtgca gcacaacgac
     4441 tccctcaatg tcacactagc ctacccagta tatgcacagc agaggcgagg atccgactac
     4501 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag
     4561 tagcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     4621 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     4681 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     4741 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     4801 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     4861 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     4921 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     4981 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     5041 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     5101 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc
     5161 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc
     5221 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc
     5281 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc
     5341 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac
     5401 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac
     5461 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag
     5521 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac
     5581 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc
     5641 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc
     5701 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac
     5761 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga
     5821 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga
     5881 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
     5941 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     6001 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     6061 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     6121 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     6181 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     6241 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     6301 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     6361 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     6421 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     6481 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     6541 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6601 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6661 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6721 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6781 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6841 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     6901 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     6961 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7021 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7081 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt
     7141 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7201 actggaaggg ctaattcact cccaacgaag ataagatctg ctttttgctt gtactgggtc
     7261 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7321 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7381 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     7441 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     7501 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7561 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7621 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     7681 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7741 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7801 ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     7861 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7921 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7981 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8041 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8101 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8161 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8221 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8281 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8341 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8401 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8461 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8521 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8581 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8641 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     8701 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8761 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8821 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8881 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8941 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9001 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9061 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9121 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9181 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9241 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9301 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9361 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9421 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9481 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9541 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9601 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9661 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9721 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9781 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9841 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9901 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9961 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    10021 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
    10081 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    10141 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
    10201 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
    10261 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
    10321 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    10381 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    10441 aacgacggcc agtgccaagc tg
//
