LOCUS       V042037                10278 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042037
VERSION     V042037
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4330
                     /label="PRKCD(NM_006254)"
                     /note="PRKCD(NM_006254)"
                     /gene="PRKCD"
     CDS             4337..4426
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        4476..4687
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4700..4968
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5003..5710
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5720..5773
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5774..6373
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6374..6961
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7035..7268
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7340..7461
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7480..7615
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7653..7669)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7677..7693
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7701..7731)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7746..7767
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8055..8643)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8814..9674)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9675..9779)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10253..10269
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggcgcc gttcctgcgc atcgccttca actcctatga
     2341 gctgggctcc ctgcaggccg aggacgaggc gaaccagccc ttctgtgccg tgaagatgaa
     2401 ggaggcgctc agcacagagc gtgggaaaac actggtgcag aagaagccga ccatgtatcc
     2461 tgagtggaag tcgacgttcg atgcccacat ctatgagggg cgcgtcatcc agattgtgct
     2521 aatgcgggca gcagaggagc cagtgtctga ggtgaccgtg ggtgtgtcgg tgctggccga
     2581 gcgctgcaag aagaacaatg gcaaggctga gttctggctg gacctgcagc ctcaggccaa
     2641 ggtgttgatg tctgttcagt atttcctgga ggacgtggat tgcaaacagt ctatgcgcag
     2701 tgaggacgag gccaagttcc caacgatgaa ccgccgcgga gccatcaaac aggccaaaat
     2761 ccactacatc aagaaccatg agtttatcgc caccttcttt gggcaaccca ccttctgttc
     2821 tgtgtgcaaa gactttgtct ggggcctcaa caagcaaggc tacaaatgca ggcaatgtaa
     2881 cgctgccatc cacaagaaat gcatcgacaa gatcatcggc agatgcactg gcaccgcggc
     2941 caacagccgg gacactatat tccagaaaga acgcttcaac atcgacatgc cgcaccgctt
     3001 caaggttcac aactacatga gccccacctt ctgtgaccac tgcggcagcc tgctctgggg
     3061 actggtgaag cagggattaa agtgtgaaga ctgcggcatg aatgtgcacc ataaatgccg
     3121 ggagaaggtg gccaacctct gcggcatcaa ccagaagctt ttggctgagg ccttgaacca
     3181 agtcacccag agagcctccc ggagatcaga ctcagcctcc tcagagcctg ttgggatata
     3241 tcagggtttc gagaagaaga ccggagttgc tggggaggac atgcaagaca acagtgggac
     3301 ctacggcaag atctgggagg gcagcagcaa gtgcaacatc aacaacttca tcttccacaa
     3361 ggtcctgggc aaaggcagct tcgggaaggt gctgcttgga gagctgaagg gcagaggaga
     3421 gtactttgcc atcaaggccc tcaagaagga tgtggtcctg atcgacgacg acgtggagtg
     3481 caccatggtt gagaagcggg tgctgacact tgccgcagag aatccctttc tcacccacct
     3541 catctgcacc ttccagacca aggaccacct gttctttgtg atggagttcc tcaacggggg
     3601 ggacctgatg taccacatcc aggacaaagg ccgctttgaa ctctaccgtg ccacgtttta
     3661 tgccgctgag ataatgtgtg gactgcagtt tctacacagc aagggcatca tttacaggga
     3721 cctcaaactg gacaatgtgc tgctggaccg ggatggccac atcaagattg ccgactttgg
     3781 gatgtgcaaa gagaacatat tcggggagag ccgggccagc accttctgcg gcacccctga
     3841 ctatatcgcc cctgagatcc tacagggcct gaagtacaca ttctctgtgg actggtggtc
     3901 tttcggggtc cttctgtacg agatgctcat tggccagtcc cccttccatg gtgatgatga
     3961 ggatgaactc ttcgagtcca tccgtgtgga cacgccacat tatccccgct ggatcaccaa
     4021 ggagtccaag gacatcctgg agaagctctt tgaaagggaa ccaaccaaga ggctgggagt
     4081 gaccggaaac atcaaaatcc accccttctt caagaccata aactggactc tgctggaaaa
     4141 gcggaggttg gagccacctt tcaggcccaa agtgaagtca cccagagact acagtaactt
     4201 tgaccaggag ttcctgaacg agaaggcgcg cctctcctac agcgacaaga acctcatcga
     4261 ctccatggac cagtctgcat tcgctggctt ctcctttgtg aaccccaaat tcgagcacct
     4321 cctggaagat ctcgaggaac aaaaactaat atcagaggaa gatttggaac agaaactaat
     4381 cagtgaagaa gatttagagc aaaagttaat ttccgaggag gacttatagg atccgcggcc
     4441 gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     4501 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
     4561 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     4621 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     4681 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg
     4741 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg
     4801 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc
     4861 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc
     4921 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc
     4981 ggcgcctacg ctagacgcca ccatggtgag caagggcgag gaggataaca tggccatcat
     5041 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     5101 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     5161 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     5221 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     5281 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     5341 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     5401 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     5461 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     5521 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     5581 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     5641 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     5701 gctgtacaag ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga
     5761 gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     5821 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     5881 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     5941 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     6001 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     6061 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     6121 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     6181 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     6241 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     6301 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     6361 gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6421 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6481 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     6541 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     6601 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     6661 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     6721 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     6781 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     6841 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     6901 tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggc cgcctccccg
     6961 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7021 agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc
     7081 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7141 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7201 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7261 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7321 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     7381 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7441 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     7501 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7561 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
     7621 ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     7681 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     7741 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7801 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7861 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7921 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7981 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8041 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8101 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8161 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8221 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8281 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8341 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8401 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8461 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     8521 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8581 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8641 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8701 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8761 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8821 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8881 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8941 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9001 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9061 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9121 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9181 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9241 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9301 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9361 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9421 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9481 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9541 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9601 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9661 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9721 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9781 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     9841 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     9901 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     9961 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
    10021 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
    10081 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
    10141 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
    10201 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
    10261 acggccagtg ccaagctg
//
