LOCUS       V035673                10088 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035673
VERSION     V035673
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4182
                     /label="PRLR(NM_000949)"
                     /note="PRLR(NM_000949)"
                     /gene="PRLR"
     promoter        4247..4458
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4471..4739
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4768..4777
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4798..5460
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5530..5583
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5584..6183
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6184..6771
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6845..7078
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7150..7271
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7290..7425
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7463..7479)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7487..7503
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7511..7541)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7556..7577
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7865..8453)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8624..9484)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9485..9589)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10063..10079
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga aggaaaatgt ggcatctgca
     2341 accgttttca ctctgctact ttttctcaac acctgccttc tgaatggaca gttacctcct
     2401 ggaaaacctg agatctttaa atgtcgttct cccaataagg aaacattcac ctgctggtgg
     2461 aggcctggga cagatggagg acttcctacc aattattcac tgacttacca cagggaagga
     2521 gagacactca tgcatgaatg tccagactac ataaccggtg gccccaactc ctgccacttt
     2581 ggcaagcagt acacctccat gtggaggaca tacatcatga tggtcaatgc cactaaccag
     2641 atgggaagca gtttctcgga tgaactttat gtggacgtga cttacatagt tcagccagac
     2701 cctcctttgg agctggctgt ggaagtaaaa cagccagaag acagaaaacc ctacctgtgg
     2761 attaaatggt ctccacctac cctgattgac ttaaaaactg gttggttcac gctcctgtat
     2821 gaaattcgat taaaacccga gaaagcagct gagtgggaga tccattttgc tgggcagcaa
     2881 acagagttta agattctcag cctacatcca ggacagaaat accttgtcca ggttcgctgc
     2941 aaaccagacc atggatactg gagtgcatgg agtccagcga ccttcattca gatacctagt
     3001 gacttcacca tgaatgatac aaccgtgtgg atctctgtgg ctgtcctttc tgctgtcatc
     3061 tgtttgatta ttgtctgggc agtggctttg aagggctata gcatggtgac ctgcatcttt
     3121 ccgccagttc ctgggccaaa aataaaagga tttgatgctc atctgttgga gaagggcaag
     3181 tctgaagaac tactgagtgc cttgggatgc caagactttc ctcccacttc tgactatgag
     3241 gacttgctgg tggagtattt agaagtagat gatagtgagg accagcatct aatgtcagtc
     3301 cattcaaaag aacacccaag tcaaggtatg aaacccacat acctggatcc tgacactgac
     3361 tcaggccggg ggagctgtga cagcccttcc cttttgtctg aaaagtgtga ggaaccccag
     3421 gccaatccct ccacattcta tgatcctgag gtcattgaga agccagagaa tcctgaaaca
     3481 acccacacct gggaccccca gtgcataagc atggaaggca aaatccccta ttttcatgct
     3541 ggtggatcca aatgttcaac atggccctta ccacagccca gccagcacaa ccccagatcc
     3601 tcttaccaca atattactga tgtgtgtgag ctggctgtgg gccctgcagg tgcaccggcc
     3661 actctgttga atgaagcagg taaagatgct ttaaaatcct ctcaaaccat taagtctaga
     3721 gaagagggaa aggcaaccca gcagagggag gtagaaagct tccattctga gactgaccag
     3781 gatacgccct ggctgctgcc ccaggagaaa accccctttg gctccgctaa acccttggat
     3841 tatgtggaga ttcacaaggt caacaaagat ggtgcattat cattgctacc aaaacagaga
     3901 gagaacagcg gcaagcccaa gaagcccggg actcctgaga acaataagga gtatgccaag
     3961 gtgtccgggg tcatggataa caacatcctg gtgttggtgc cagatccaca tgctaaaaac
     4021 gtggcttgct ttgaagaatc agccaaagag gccccaccat cacttgaaca gaatcaagct
     4081 gagaaagccc tggccaactt cactgcaaca tcaagcaagt gcaggctcca gctgggtggt
     4141 ttggattacc tggatcccgc atgttttaca cactcctttc actgaggatc cctgcgatat
     4201 ccagcgcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca
     4261 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga
     4321 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     4381 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     4441 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc
     4501 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
     4561 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc
     4621 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
     4681 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc
     4741 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc
     4801 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg
     4861 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa
     4921 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac
     4981 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc
     5041 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc
     5101 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc
     5161 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg
     5221 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac
     5281 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc
     5341 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc
     5401 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc
     5461 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc
     5521 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
     5581 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     5641 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     5701 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     5761 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     5821 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     5881 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     5941 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     6001 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     6061 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     6121 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     6181 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     6241 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     6301 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6361 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     6421 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6481 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6541 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     6601 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6661 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6721 ctccgccttc gccctcagac gagtcggatc tccctttggc cgcctccccg cctggtacct
     6781 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     6841 ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg
     6901 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6961 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7021 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7081 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     7141 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7201 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     7261 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc
     7321 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     7381 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag
     7441 acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7501 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7561 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7621 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7681 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7741 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7801 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7861 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7921 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7981 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8041 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8101 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8161 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8221 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8281 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     8341 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8401 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8461 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8521 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8581 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8641 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8701 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8761 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8821 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8881 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8941 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9001 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9061 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9121 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9181 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9241 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9301 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9361 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9421 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9481 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9541 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9601 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     9661 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     9721 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     9781 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     9841 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     9901 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     9961 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
    10021 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
    10081 ccaagctg
//
