LOCUS       V034590                 9714 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034590
VERSION     V034590
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..3909
                     /label="PRMT3(NM_005788)"
                     /note="PRMT3(NM_005788)"
                     /gene="PRMT3"
     CDS             3916..3942
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3946..3972
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3976..4002
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4067..4278
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4291..4559
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4588..4597
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4618..5280
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5350..5403
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5404..5802
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     misc_feature    5809..6397
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6471..6704
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6776..6897
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6916..7051
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7089..7105)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7113..7129
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7137..7167)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7182..7203
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7491..8079)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8250..9110)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9111..9215)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9689..9705
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt gctcgttagc gtcaggcgct
     2341 accggcggcc ggggcgctgt ggagaatgag gaggacctgc cagaactgtc ggacagcggg
     2401 gacgaggccg cctgggagga tgaggacgat gcagatctcc cccacggcaa gcagcagacc
     2461 ccctgcctgt tctgtaacag gttattcaca tctgctgaag aaacattttc acactgtaag
     2521 tctgagcatc agtttaatat tgacagcatg gttcataaac atggacttga attttatgga
     2581 tacattaagc taataaattt tattagactt aagaatccta cagttgagta catgaattcc
     2641 atatacaacc cagtgccttg ggagaaagaa gagtatttga agccagtatt agaagatgac
     2701 cttttacttc aatttgatgt agaagatctt tatgaaccgg tgtcagtacc cttctcatac
     2761 cccaatggac tcagtgaaaa tacatctgtt gttgaaaaat tgaaacatat ggaagccagg
     2821 gcactgtctg ctgaagccgc attggccaga gcacgtgagg atctgcaaaa aatgaaacaa
     2881 tttgctcagg attttgtgat gcacacagat gtcagaacct gctcgtcatc tactagtgtc
     2941 attgcggacc tccaggagga tgaggatggt gtttatttca gctcatacgg gcattatggg
     3001 atacatgaag aaatgctaaa ggacaaaata cgaacagaaa gctaccgaga tttcatatac
     3061 caaaatccac atatcttcaa agacaaggta gttttggatg ttgggtgtgg aactggaatt
     3121 ctctctatgt ttgctgctaa agctggggcg aagaaggttc ttggagttga tcaatctgaa
     3181 atactttacc aggcaatgga tattataaga ctaaataaac ttgaagatac tattacacta
     3241 attaaaggaa agattgaaga agttcatctt cctgtagaaa aagtagatgt tatcatatct
     3301 gagtggatgg gctattttct tctgtttgag tctatgttag attctgtcct ttatgcaaag
     3361 aacaaatact tggcaaaagg aggctcggtc taccctgaca tttgcactat cagccttgta
     3421 gcagtgagtg atgtgaataa acatgctgat agaattgctt tttgggatga tgtctatggc
     3481 ttcaagatgt cctgcatgaa gaaagcagtt attccagaag ctgttgtgga agttttagat
     3541 ccgaagactc ttatttcaga accttgtggt attaagcata tagattgcca tacgacgtct
     3601 atctcagatt tggaattttc atcagatttt accctgaaaa tcacaaggac atccatgtgc
     3661 acggcaattg ctggctactt tgatatatat tttgagaaga attgccacaa cagggtcgtg
     3721 ttctctacgg gccctcagag caccaaaaca cactggaaac aaacagtatt tctactggaa
     3781 aaaccatttt cagttaaagc aggtgaagcc ttgaaaggaa aggtcacagt tcacaagaat
     3841 aagaaagatc cacgttctct caccgtgacc ctcacgttga ataattcaac tcaaacttat
     3901 ggtctccagc tcgagtaccc atacgacgta cctgattatg caacttatcc ttatgatgta
     3961 cctgattatg ctagctatcc ttatgatgtc ccagactacg catagggatc cctgcgatat
     4021 ccagcgcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca
     4081 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga
     4141 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     4201 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     4261 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc
     4321 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
     4381 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc
     4441 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
     4501 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc
     4561 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc
     4621 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg
     4681 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa
     4741 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac
     4801 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc
     4861 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc
     4921 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc
     4981 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg
     5041 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac
     5101 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc
     5161 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc
     5221 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc
     5281 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc
     5341 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
     5401 cctatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca
     5461 atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac
     5521 ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc
     5581 gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc
     5641 ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat
     5701 ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg
     5761 attcgtgaat tgctgccctc tggttatgtg tgggagggct aagtcgacaa tcaacctctg
     5821 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     5881 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     5941 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6001 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6061 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6121 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6181 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     6241 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     6301 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     6361 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac
     6421 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6481 aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga
     6541 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6601 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6661 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     6721 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     6781 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6841 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6901 tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6961 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7021 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg
     7081 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7141 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7201 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7261 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7321 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7381 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7441 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7501 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7561 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7621 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7681 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7741 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7801 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7861 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7921 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7981 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8041 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8101 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8161 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8221 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8281 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8341 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8401 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8461 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8521 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8581 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8641 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8701 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8761 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8821 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8881 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8941 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9001 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9061 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9121 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9181 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9241 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     9301 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     9361 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     9421 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     9481 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     9541 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     9601 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     9661 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
