LOCUS       V037439                11045 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037439
VERSION     V037439
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5139
                     /label="PRPF6(NM_012469)"
                     /note="PRPF6(NM_012469)"
                     /gene="PRPF6"
     promoter        5204..5415
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5428..5696
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5725..5734
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5755..6417
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6487..6540
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6541..7140
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7141..7728
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7802..8035
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8107..8228
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8247..8382
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8420..8436)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8444..8460
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8468..8498)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8513..8534
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8822..9410)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9581..10441)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10442..10546)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11020..11036
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga acaagaagaa gaaaccgttc
     2341 ctagggatgc ccgcgcccct cggctacgtg ccggggctgg gccggggcgc cactggcttc
     2401 accacgcggt cagacattgg gcccgcccgt gatgcaaatg accctgtgga tgatcgccat
     2461 gcacccccag gcaagagaac cgttggggac cagatgaaga aaaatcaggc tgctgacgat
     2521 gacgacgagg atctaaatga caccaattac gatgagttta atggctatgc tgggagcctc
     2581 ttctcaagtg gaccctacga gaaagatgat gaggaagcag atgctatcta tgcagccctg
     2641 gataaaagga tggatgaaag aagaaaagaa agacgggagc aaagggagaa agaagaaata
     2701 gagaaatatc gtatggaacg ccccaaaatc caacagcagt tctcagacct caagaggaag
     2761 ttggcagaag tcacagaaga agagtggctg agcatccccg aggttggcga tgccagaaat
     2821 aaacgtcagc ggaacccacg ctatgagaag ctgacccctg ttcctgacag tttctttgcc
     2881 aaacatttac agaccggaga gaaccatacc tcagtggatc cccgacaaac tcaatttgga
     2941 ggtcttaaca caccctatcc aggtggacta aacactccat acccaggtgg aatgacgcca
     3001 ggactgatga cacctggcac aggtgagctg gacatgagga agattggcca agcgaggaac
     3061 actctgatgg acatgaggct gagccaggtg tctgactccg tgagtggaca gaccgtcgtt
     3121 gaccccaaag gctacctgac ggatttaaat tccatgatcc cgacacacgg aggagacatc
     3181 aatgatatca agaaggcgcg actgctcctc aagtctgttc gggagacgaa ccctcatcac
     3241 ccgccagcct ggattgcatc agcccgcctg gaagaagtca ctgggaagct acaagtagct
     3301 cggaacctta tcatgaaggg gacggagatg tgccccaaga gtgaagatgt ctggctggaa
     3361 gcagccaggt tgcagcctgg ggacacagcc aaggccgtgg tagcccaagc tgtccgtcat
     3421 ctcccacagt ctgtcaggat ttacatcaga gccgcagagc tggaaacgga cattcgtgca
     3481 aagaagcggg ttcttcggaa agccctcgag catgttccaa actcggttcg cttgtggaaa
     3541 gcagccgttg agctggaaga acctgaagat gctagaatca tgctgagccg agctgtggag
     3601 tgctgcccca ccagcgtgga gctctggctt gctctggcaa ggctggagac ctatgaaaat
     3661 gcccgcaagg tcttgaacaa ggcgcgggag aacattccta cagaccgaca tatctggatc
     3721 acggctgcta agctggagga agccaatggg aacacgcaga tggtggagaa gatcatcgac
     3781 cgagccatca cctcgctgcg ggccaacggt gtggagatca accgtgagca gtggatccag
     3841 gatgccgagg aatgtgacag ggctgggagt gtggccacct gccaggccgt catgcgtgcc
     3901 gtgattggga ttgggattga ggaggaagat cggaagcata cctggatgga ggatgctgac
     3961 agttgtgtag cccacaatgc cctggagtgt gcacgagcca tctacgccta cgccctgcag
     4021 gtgttcccca gcaagaagag tgtgtggctg cgcgccgcgt acttcgagaa gaaccatggc
     4081 actcgggagt ccctggaagc actcctgcag agggctgtgg cccactgccc caaagcagag
     4141 gtgctgtggc tcatgggcgc caagtccaag tggctggcag gggatgtgcc tgcagcaagg
     4201 agcatcctgg ccctggcctt ccaggccaac cccaacagtg aggagatctg gctggcagcc
     4261 gtgaagctgg agtccgagaa tgatgagtac gagcgggccc ggaggctgct ggccaaggcg
     4321 cggagcagtg cccccaccgc ccgggtgttc atgaagtctg tgaagctgga gtgggtgcaa
     4381 gacaacatca gggcagccca agatctgtgc gaggaggccc tgcggcacta tgaggacttc
     4441 cccaagctgt ggatgatgaa ggggcagatc gaggagcaga aggagatgat ggagaaggcg
     4501 cgggaagcct ataaccaggg gttgaagaag tgtccccact ccacacccct gtggcttttg
     4561 ctctctcggc tggaggagaa gattgggcag cttactcgag cacgggccat tttggaaaag
     4621 tctcgtctga agaacccaaa gaaccctggg ctgtggttgg agtccgtgcg gctggagtac
     4681 cgtgcggggc tgaagaacat cgcaaataca ctcatggcca aggcgctgca ggagtgcccc
     4741 aactccggta tcctgtggtc tgaggccatc ttcctcgagg caaggcccca gaggaggacc
     4801 aagagcgtgg atgccctgaa gaagtgtgag catgaccccc atgtgctcct ggccgtggcc
     4861 aagctgtttt ggagtcagcg gaagatcacc aaggccaggg agtggttcca ccgcactgtg
     4921 aagattgact cggacctggg ggatgcctgg gccttcttct acaagtttga gctgcagcat
     4981 ggcactgagg agcagcagga ggaggtgagg aagcgctgtg agagtgcaga gcctcggcat
     5041 ggggagctgt ggtgcgccgt gtccaaggac atcgccaact ggcagaagaa gatcggggac
     5101 atccttaggc tggtggccgg ccgcatcaag aacaccttct gaggatccct gcgatatcca
     5161 gcgcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     5221 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     5281 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     5341 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     5401 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     5461 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     5521 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     5581 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     5641 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     5701 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc
     5761 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc
     5821 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc
     5881 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc
     5941 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac
     6001 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac
     6061 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag
     6121 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac
     6181 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc
     6241 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc
     6301 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac
     6361 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga
     6421 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga
     6481 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
     6541 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     6601 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     6661 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     6721 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     6781 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     6841 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     6901 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     6961 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     7021 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     7081 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     7141 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7201 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7261 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7321 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7381 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7441 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7501 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     7561 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7621 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc
     7681 cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta
     7741 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     7801 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct
     7861 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7921 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7981 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     8041 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     8101 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8161 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8221 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt
     8281 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     8341 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg
     8401 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8461 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8521 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8581 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8641 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8701 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8761 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8821 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8881 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8941 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9001 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9061 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9121 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9181 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9241 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     9301 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9361 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9421 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9481 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9541 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9601 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9661 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9721 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9781 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9841 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9901 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9961 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10021 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10081 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10141 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10201 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10261 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10321 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10381 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10441 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10501 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10561 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
    10621 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
    10681 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
    10741 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
    10801 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
    10861 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    10921 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    10981 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
    11041 agctg
//
