LOCUS       V029241                12019 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029241
VERSION     V029241
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6045
                     /label="Pitpnm1(NM_008851)"
                     /note="Pitpnm1(NM_008851)"
                     /gene="Pitpnm1"
     CDS             6052..6117
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6161..6372
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6385..6653
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6682..6691
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6712..7374
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7444..7497
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7498..8097
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8098..8686
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8760..8993
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9065..9186
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9226..9361
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9394..9410)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9418..9434
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9442..9472)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9487..9508
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9796..10384)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10555..11415)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11416..11520)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11994..12010
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc ttatcaagga gtaccacatc
     2341 ctgctgccca tgagcctgga cgagtatcaa gtggcccagc tctacatgat ccagaaaaag
     2401 agccgtgagg agtctagtgg tgagggcagc ggcgtggaga tcctggccaa ccggccctac
     2461 acagatgggc ctggaggcaa cgggcagtat acacacaagg tgtaccacgt gggctcccac
     2521 atcccaggct ggttccgggc actgttaccc aaggctgctc tgcaggtaga agaggaatct
     2581 tggaacgctt acccatatac ccggacacgg tacacctgcc cctttgtgga gaagttctcc
     2641 attgagatag agacctacta cttgcctgat ggggggcagc aacctaacgt cttcaacctg
     2701 agtggggctg agagaagaca gagaatcgtg gataccatcg acatcgtgcg ggatgcagtg
     2761 gccccaggag aatacaaagc ggaagaggac cctcggctgt accgctcagc caagacaggc
     2821 cgagggccgc tggctgatga ctgggcacgg acagcggccc agacaggacc tctcatgtgt
     2881 gcctataagc tgtgcaaggt tgaattccgc tactggggca tgcaggccaa gattgagcag
     2941 ttcatccatg acgtaggtct gcgcagggtg atgcttcgcg cccatcgcca ggcctggtgt
     3001 tggcaagatg agtggataga actgagcatg gctgacatcc gggcactgga ggaggagact
     3061 gcacgcatgc tagcgcagcg tatggctaag tgcaacactg gcagtgaggg accagaggct
     3121 cagacccctg ggaaatccag cactgaggcc cgacctggga ccagcactgc tggcacccct
     3181 gatggacctg aggcccctcc cggccccgac gcctccccag atgccagctt tgggaagcag
     3241 tggtcctcat cctcccgttc ctcctactca tcccaacatg gaggcggcgt gtctccacag
     3301 agcttgtctg agtggcgcat gcagaacatt gcccgagact ctgagaacag ctccgaggaa
     3361 gaattctttg atgcccatga aggtttctcg gacagtgatg aggtcttccc caaggagatg
     3421 accaagtgga attccaatga ttttatcgat gcctttgcct ccccaaccga ggtggagggg
     3481 gtaccagatc ctacagtcat ggctaccaaa ggcattgaag atggggcccg agctcccagg
     3541 gactcagagg gcctagatgg agcgggggat ctagtggtcg aggcgtgctc tgtgcatgcc
     3601 ctcttcctca tcctgcacag cggcagcatc ctggactctg gccctggaga caccaactcc
     3661 aagcaggccg acgtgcaaac gctgagcaca gcctttgagg ccgtcacccg agtccatttc
     3721 cccgaggccc tgggtcacgt ggcactgcgg ctggtgccct gtccacccat ttgcgcggct
     3781 gcctatgctc ttgtctccaa cctgagcccc tacagccatg atggggatag cctgtcccgc
     3841 tcccaagacc acattccgct ggctgccctg ccgctcctgg ccacctcatc ctctcgctac
     3901 cagggtgccg tggccactgt catcgctcgc accaaccagg cctatgcagc cttcctgcgc
     3961 tcatcggagg gcacaggttt ctgcgggcag gtggtgctga tcggagatgg tgttggtggc
     4021 atcctgggct ttgatgcgct ctgccacagt gccagtgcag gcccagggag tcggggcagc
     4081 agccgccgtg ggagcatgaa caatgagatg ctctcccctg aagttggtcc agtacgggac
     4141 ccgctggcag atggggtgga ggtactgggt cgtgctagcc cagaaccctc agccctacct
     4201 gctcagcgta ccttcagtga catggccaat cctgatcctg atggctctca gaacagcctc
     4261 caggtagcct ccacagctac ctcctctggg gagccccgcc gtgcaagcac agcctcctgt
     4321 ccacctgcca gttctgaggc tcctgacggc cccacgaacg ctgctcgcct ggacttcaag
     4381 gtctcaggct tcttcctctt tggctcccca ctgggcctgg tgctggctct tcgcaaaacc
     4441 gtgatgcccg ccttggaggt ggctcagctg cgtccagcct gcgagcagat ctacaatctc
     4501 ttccatgccg ctgacccctg tgcctcccgc ctggaacccc tgctggcccc caagttccag
     4561 gccatcgccc ccctggctgt acctcgttac cagaagtttc ccctaggaga cggctcgtcc
     4621 ttgctgctgg ctgacacttt gcaaacccat tcgagtctct tcctggaaga gctggagatg
     4681 atggtgccct ccacacccac ctcggccagt ggtgccttct ggaagggcag tgaactaggc
     4741 aatgagccag catcccagac agcagccccc agcaccacca gcgaggttgt taagatcctg
     4801 gaccgctggt ggggaaacaa gcggatcgac tattcactgt actgccccga ggcactcacg
     4861 gccttcccca cggtcacgct gccccacctc ttccacgcca gctactggga atcagcggat
     4921 gtagtggcct tcattctgcg ccaggtcatt gagaaggagc ggccacagct gacagagtgt
     4981 gaggagccat ccatctatag ccccgccttc cccagggaga agtggcagcg caaacgcaca
     5041 caggtcaaga tccggaacgt cacttccaac caccgagcga gtgacaccgt ggtatgtgag
     5101 ggccgtcccc aggtgctgaa tgggcgcttc atgtatggac cattggatgt ggtcacgctc
     5161 actggagaga aggtggatgt ctacgtcatg acacagccac tgtcaggcaa gtggatccac
     5221 tttggcacag aggtcactaa cagctcaggc cgtctcacct tcccagtgcc ctcagaacgt
     5281 gcactgggca ttggtgtcta ccctgtgcgc atggtggtca ggggagacca cacctatgcc
     5341 gagtgctgtc tgactgtggt gtcccgaggc acagaagctg tggtcttcag catcgatggt
     5401 tctttcactg ccagtgtctc tatcatgggc agcgacccca aggtgcgcgc tggcgcagtg
     5461 gacgtggtca ggcactggca ggactctggc tacttgattg tgtatgtaac tggccggcct
     5521 gacatgcaaa agcaccgtgt agtggcctgg ctgtcgcaac acaacttccc ccatggtgtt
     5581 gtctccttct gtgatggcct cacccacgac ccgctgcggc agaaagccat gtttctgcag
     5641 agcctggtgc aagaggtaga actgaacatc gtggctggat atgggtcacc aaaagatgtg
     5701 gcagtgtatg cagcactggg gctctctccg agccagacct acattgttgg ccgtgctgtg
     5761 cgcaaactgc aggcacagtg tcagttcctg tcagatggct atgtggccca cttgggccag
     5821 ctggaggcag gctcccactc ccatgctccc tcaggacctc cgagagccgc tctggccaag
     5881 agtagctatg ctgtggctgc ccctgtggac ttcctccgga agcagagcca gctgcttcga
     5941 tccagaggcc ctagccaggt ggaccgtgag ggtccaggaa cacctcccac caccctggcc
     6001 aggggcaaga ctcgcagcat cagcctcaag ttggacagtg aagagggatc cgactacaaa
     6061 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtag
     6121 cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     6181 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     6241 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     6301 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     6361 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     6421 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     6481 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     6541 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     6601 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     6661 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg
     6721 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc
     6781 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc
     6841 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc
     6901 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc
     6961 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc
     7021 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac
     7081 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc
     7141 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc
     7201 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc
     7261 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc
     7321 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc
     7381 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct
     7441 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg
     7501 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     7561 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     7621 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     7681 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     7741 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     7801 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     7861 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     7921 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     7981 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     8041 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat
     8101 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     8161 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     8221 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     8281 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     8341 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     8401 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     8461 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     8521 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     8581 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     8641 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag
     8701 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     8761 ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta ctgggtctct
     8821 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8881 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8941 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     9001 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     9061 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9121 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9181 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     9241 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     9301 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9361 ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
     9421 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     9481 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     9541 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     9601 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     9661 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     9721 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9781 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9841 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9901 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9961 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    10021 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    10081 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    10141 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    10201 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
    10261 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    10321 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    10381 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    10441 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    10501 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    10561 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    10621 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    10681 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    10741 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10801 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10861 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10921 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10981 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    11041 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    11101 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    11161 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    11221 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    11281 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    11341 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    11401 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    11461 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    11521 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    11581 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    11641 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    11701 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    11761 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    11821 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    11881 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    11941 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    12001 gacggccagt gccaagctg
//
