LOCUS       V024555                14747 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024555
VERSION     V024555
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..8790
                     /label="Prrc2a(NM_020027)"
                     /note="Prrc2a(NM_020027)"
                     /gene="Prrc2a"
     CDS             8797..8862
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        8906..9117
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             9130..9398
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      9427..9436
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             9457..10119
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             10189..10242
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             10243..10842
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    10843..11430
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             11504..11737
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    11809..11930
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      11949..12084
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(12122..12138)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    12146..12162
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(12170..12200)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    12215..12236
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12524..13112)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(13283..14143)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(14144..14248)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     14722..14738
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt ccgatcgctc ggggccgact
     2341 gccaagggga aggatggaaa gaagtattcc tcgctcaacc tgtttgatac gtataagggc
     2401 aagtccttag agatccagaa acccgctgtt gcccctcgac atggcctgca gagtctcgga
     2461 aaagttgcca ttgcccggcg catgccaccc ccagccaacc ttccaagcct gaaagccgag
     2521 aacaaaggca atgaccccaa tgtctcacta gtgccgaaag acggaacagg atgggcaagc
     2581 aaacaggagc agtccgaccc caagagttcc gatgcctcaa ccgctcagcc gccggaatcg
     2641 cagccactgc cggcttcaca gacgcctgcc tccaaccagc cgaaacgacc cccaacagcc
     2701 cccgagaaca ctccttcggt tccaagcggg gtaaagtcct gggcacaagc cagtgtcacc
     2761 catggagccc atggagatgg tggaagggca tcaaacctac tgtcgcgatt ctctcgagag
     2821 gaatttccga ccctgcaggc ggctggagac caggacaagg ctgccaagga aagggagtct
     2881 gccgaacagt cgtctgggcc cggaccaagc ctccgccccc aaaattctac aacttggagg
     2941 gacggaggtg ggcgtggccc tgatgacctg gagggcccgg actccaaact tcatcatggc
     3001 catgatcccc ggggtgggct gcagccttca ggcccacccc agttccctcc ctaccgcggg
     3061 atgatgccgc cctttatgta tcccccatat ctcccattcc ctccgcccta tggaccccag
     3121 gggccttata gatacccgac tcctgatggg cccagccgtt ttccccgtgt ggcgggcccc
     3181 cggggctcag ggccaccaat gcgccttgta gagcctgtgg gtcggccttc tattcttaaa
     3241 gaggataatc ttaaagagtt tgaccaactg gaccaggaaa atgatgatgg ttgggcaggg
     3301 gcccatgaag aggttgacta tactgaaaag ctcaaattca gcgatgagga agatggacga
     3361 gactccgacg aggagggagc tgagggccac aaggattccc agtcagctgc tgctgaggaa
     3421 cctgagacag atggcaaaaa gggcacctcc cctggcagcg aattgccccc tcccaagaca
     3481 gcctggacag agaatgctcg gccttcagag acagagccag ctcctcctac cccaaagcct
     3541 cccccacctc cacctcaccg ggggccagtc gggaactggg ggccccctgg ggactaccca
     3601 gatcgagggg gtcctccctg caaaccccca gcacctgagg atgaggatga ggcatggcgg
     3661 cagcgtcgga agcaatcttc ttcagagatc tccctggcag tggaacgagc tcgaaggcgc
     3721 agagaagagg aagagcgacg catgcaggag gagcgccggg cagcctgtgc tgagaaactt
     3781 aagcgtcttg atgaaaagtt tggggcacct gacaagcggc tcaaggcaga gcctgcagcc
     3841 ccacctgtta cccctgctgc cccagctcta ccacctgtag tccccaaaga aattcctgca
     3901 gctccagctc tgccaccaac acctacccca acaccagaga aagaacctga agagccggca
     3961 caagcccctc ctgtgcaggc cgcccccagt ccaggggtgg ctccagttcc cactctggtg
     4021 agtgggggtg gttgcactgc caacagcaac agcagtggca gcttcgaagc cagcccagtg
     4081 gaaccccaac tgccttcaaa agagggtcct gaaccaccag aagaggttcc tccacctacc
     4141 acacccccag ccccaaagat ggaacccaaa ggtgatgggg ttggttctac tcgccagccc
     4201 cccagtcagg gcttgggcta ccccaaatac cagaagtcat tgcctcctcg cttccagcgg
     4261 cagcaacagg agcagctcct gaagcagcag cagcagcaac agcagtggca gcagcagcaa
     4321 caaggcactg cccctcctgc cccagtgccc ccatcaccac cacaacctgt gaccctggga
     4381 gctgtacctg ccccacaagc accgccccca cctcccaaag ccctgtaccc aggtgctctg
     4441 ggccggcccc caccaatgcc tccaatgaac tttgaccccc gctggatgat gattcctcct
     4501 tacgtggacc ctcggcttct ccagggccgg ccccctctgg acttctaccc tcctggtgtg
     4561 catccctctg gcctggttcc ccgggaacgc tcagacagtg ggggctcaag ctcagagcca
     4621 tttgaacgcc atgcaccccc tctgttgcgg gaaaggggca ccccaccagt ggaccccaag
     4681 ttggcctggg ttggagatgt ctttaccacc acacccactg atcctcgtcc tcttacctca
     4741 ccactgcgcc aggcagcaga tgaggaggag aagagtatga ggagcgagac tcctccggta
     4801 cccccgcccc caccttatct ggctaattat ccaggttttc cagagaatgg aactcctggg
     4861 cccccaatct cgagattccc tctggaggaa tctgctcccc cgggaccccg cccactaccc
     4921 tggcctccag gcaatgatga agctgccaag atgcaggctc caccgcccaa gaaggaaccc
     4981 tcgaaggagg aaccgccaca gctgtccggg ccagaagcag gcagaaagcc tgcccgtgga
     5041 ggccaaggcc caccaccacc acggagagag aatcgtactg agactcgctg gggcccccga
     5101 ccaggcagtt gtcgccgtgg aatccctcca gaggagccag gtgtccctcc tcgaagagct
     5161 gggcctataa agaagccacc accacctgtg aaagtagagg agctgcctcc taagtcccta
     5221 gaacaggggg atgagacccc caaggtccca aagccagatg cactcaagac agctaagggc
     5281 aaggtgggcc ccaaagaaac cccaccaggt ggaaacctgt ctcctgcacc taggcttcgg
     5341 cgggactatt cctatgaaag agtaggtcct acatcttgcc gtggtcgggg cagaggcgag
     5401 tattttgcca gaggaagggg ttttcggggg acctatgggg gccggggtcg gggagcccgg
     5461 agccgtgaat tccgaagtta ccgagagttc cgaggagacg atgggcgtgg gggtggttca
     5521 ggcggaacaa accatccttc tgctccccga ggccgcactg ccagtgagac acgaagtgag
     5581 ggttcagaat atgaagaaat ccccaagaga cgccgccagc gtggctctga aacaggcagt
     5641 gagactcatg agagtgacct ggccccctct gacaaggaag cccctccgcc caaagaggga
     5701 gtgcttggtc aggtccctct tgccccccca caaccaggag ctcctccatc accagcccca
     5761 gcccgattct ccactgcccg aggtggtcgt gtcttcactc caagaggagt accatcacgc
     5821 cgtggccgag gtggggggcg gccccctcct gtttgctcag gctggagccc tcctgccaag
     5881 tctctggttc caaagaaacc tccaacaggt cctttgcctc caagtaaaga acctttgaaa
     5941 gagaagctga tctcagggcc tctgtccccc atgtctcgag ctggcaacat gggtgtaggc
     6001 atggaagatg gggagcgacc ccgacggagg cgacatggca gggctcaaca gcaggacaaa
     6061 ccacctcgtt tccgaaggct gaaacaagaa cgtgaaaacg ctgccagagg agctgatggc
     6121 aagccccctt ccctaaccct tgcagcctct acccctggac ctgaggagac actaacagca
     6181 gccacagttc ccccaccccc tcgccgcaca gctgctaagt ctcctgatct gtcaaaccag
     6241 aactcagacc aagccaatga ggaatgggag actgcatcag agagcagcga ctttgccagt
     6301 gagcgccggg gtgacaagga gactccccca gcagcactta tgacctctaa agctgtgggg
     6361 actcctgggg ccaatgcagg tggggctgga ccaggcattt cagccatgtc tcgtggagac
     6421 ctgagccaac gagccaagga tttgagcaag cggagcttct caagtcagcg gccaggcatg
     6481 gatcgtcaaa accggcgccc aggcactggg ggcaagactg gcagtggtgg tggcagtagc
     6541 ggaggtggtg gtgcaggccc tgggggacgg accggcccag gacgagggga taagaggagc
     6601 tggccctctc ccaagaaccg aagccgtcct cctgaggagc gacctccggg acttcctctg
     6661 cctccgccac cccccagcag ttctgctgtc ttccgcctgg accaagttat ccatagcaac
     6721 cctgctggaa tccaacaggc tctggcccaa cttagcagcc gccaaggaaa tgtaactgca
     6781 ccaggggggc acccaaggcc taagcctggg cctccccaag cccctcaggg ctcctcccct
     6841 cggcccccaa cccgttatga ccccccaagg gccagcagcg ctatcagttc tgacccccac
     6901 ttcgaggagc cagggccgat ggtgaggggg gtgggcggga ctccccggga ctctgccggg
     6961 gttaatccct tccccccgaa acggcgggag cggcctcccc gaaaaccgga gctgctgcag
     7021 gaggaaactg tgccagcttc tcatagctct ggattcttgg gctctaagcc tgaggtccca
     7081 ggccctcagg aagaatccag agattcaggc acagaggccc taactcccca catctggaat
     7141 cgtttacata ctgccacaag ccgaaagagt taccagcctg gctccatcga gccctggatg
     7201 gagcccctga gtccctttga ggatgtggca ggcacagaga tgagtcagtc tgacagtgga
     7261 gtggacctga gtggagattc tcaggtgtca tcaggtccct gcagtcagcg aagttcccct
     7321 gatggaggac ttaaggggtc agcagaggga ccccccaagc ggcctggagg accctcaccc
     7381 ctgaatgctg ttcctggtga gagtgcatct ggctctgaac cctctgaacc tcctaggaga
     7441 cggccacctg cttcccatga aggggaaaga aaggagctgc cccgggagca gcctctgccc
     7501 cctggtccca taggcacaga acgatcacag cgcacagatc gaggcccaga gccaggccct
     7561 ctccgaccag cccaccgacc gggctctcaa gttgagtttg gcactaccaa caaggactca
     7621 gacttgtgcc tagtggtcgg agacacttta aaaggagaaa aggagttggt ggcatcagcc
     7681 acagaggccg ttcccatatc cagagactgg gagctgctcc ctagtgcttc tacttcagct
     7741 gagccacagc ccaagagcct gggttctggg cagtgtgttc cagagcccag cccttcaggt
     7801 cagcgtccgt accctgaggt cttctatggc agccctggac ctcctaattc ccagcaggtc
     7861 tctgggggtg cacccatcga ctctcagtta catcccaaca gtggaggctt ccgtcctggg
     7921 acaccctcat tgcaccagta caggtcacag cctctgtatc tgcccccagg tccagcccct
     7981 ccctcggcac tgctttctgg ggtagctctc aagggccagt ttctggattt ctcagcactg
     8041 caagccacag agctggggaa gttgcctgct gggggagttc tctaccctcc gccatccttc
     8101 ctctactctg cagctttctg ccccagccct ttgcctgacc cacccttgct tcaggtacgc
     8161 caggatctgc catccccttc agatttttac tctactcctc tgcagcctgg tggccagagt
     8221 ggcttccttc cttcgggagc ccctgctcag cagatgcttc tacctgtggt agactcacag
     8281 ctgccagtgg tgaactttgg ctccctgcca ccagcgccac cccctgctcc accccccctt
     8341 tctctgttac ctgtgggtcc tgctctccag ccccccaacc tagctgtgcg gcccccacct
     8401 gctcctgctg ctcgggtctt accttcaccc gctagaccct tcgcccctag cttggggcga
     8461 gcagagctgc acccagtaga actaaagcct ttccaggatt accgaaagct gagcagcaac
     8521 cttgggggtc ctgggtcgtc acggactccc ccatctggaa ggcccttctc tggtctcaat
     8581 tcccgtctca aggccccacc ttccacctac agtggagtat tccgaactca gcgcattgac
     8641 ctctaccagc aggcttcccc accagatgcc ctgcgctgga tgccgaagcc ttgggaacgg
     8701 gcagggccac cttctcgaga agggcctccc cgaagagcag aggagcctgg gtcccggggg
     8761 gagaaggagc ctggattgcc cccaccccgc ggatccgact acaaagacca tgacggtgat
     8821 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc gcgaaggatc
     8881 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     8941 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     9001 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     9061 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     9121 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     9181 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     9241 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     9301 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     9361 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     9421 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
     9481 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
     9541 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
     9601 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
     9661 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
     9721 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
     9781 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
     9841 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
     9901 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
     9961 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
    10021 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
    10081 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc
    10141 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga
    10201 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
    10261 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
    10321 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
    10381 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
    10441 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
    10501 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
    10561 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
    10621 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
    10681 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
    10741 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
    10801 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac
    10861 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
    10921 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
    10981 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
    11041 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
    11101 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
    11161 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
    11221 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
    11281 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
    11341 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg
    11401 agtcggatct ccctttggcc gcctccccgc ctggtacctt taagaccaat gacttacaag
    11461 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
    11521 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
    11581 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
    11641 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
    11701 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
    11761 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
    11821 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    11881 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    11941 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
    12001 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
    12061 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat
    12121 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    12181 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    12241 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    12301 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    12361 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    12421 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    12481 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    12541 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    12601 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    12661 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    12721 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    12781 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    12841 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    12901 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    12961 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    13021 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    13081 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    13141 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    13201 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    13261 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    13321 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    13381 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    13441 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    13501 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    13561 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    13621 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    13681 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    13741 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    13801 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    13861 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    13921 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    13981 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    14041 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    14101 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    14161 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    14221 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
    14281 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
    14341 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
    14401 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
    14461 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
    14521 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
    14581 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
    14641 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    14701 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
