LOCUS       V045246                12775 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045246
VERSION     V045246
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6801
                     /label="Pzp(NM_007376)"
                     /note="Pzp(NM_007376)"
                     /gene="Pzp"
     CDS             6808..6873
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6917..7128
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7141..7409
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7438..7447
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7468..8130
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8200..8253
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8254..8853
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8854..9442
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9516..9749
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9821..9942
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9982..10117
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10150..10166)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10174..10190
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10198..10228)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10243..10264
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10552..11140)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11311..12171)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12172..12276)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12750..12766
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga ggagaaacca gctgcccaca
     2341 ccagcttttc ttttactgtt cctgcttctt cccagagatg ccaccacagc tactgcaaaa
     2401 ccacaatatg tggtgctggt cccgtcagag gtctattcag gagtccctga aaaggcctgt
     2461 gtcagcctca accatgtgaa tgagactgtg atgctcagct taactctaga gtatgcaatg
     2521 cagcaaacga agctcctcac agaccaggct gtggataagg actccttcta ctgcagcccc
     2581 ttcacgatct caggttcacc tttaccctac acattcatta ctgtggagat aaaaggacca
     2641 acgcagcgct tcataaagaa gaagtcaata caaataataa aagctgagag cccagtcttt
     2701 gtccagacag acaaacccat atacaaacca ggacagatag tgaaattccg agttgtttct
     2761 gtggacatca gttttcgccc gttgaatgaa acgttccctg tcgtttatat tgagactccc
     2821 aagaggaacc gaatttttca atggcaaaat atccatctgg caggaggact ccaccagctc
     2881 tctttcccac tgtctgttga gccagctctg ggtatctaca aggttgtagt gcagaaggac
     2941 tcagggaaga aaatagaaca ctcctttgag gtgaaggaat acgttttacc caaatttgag
     3001 gtgataataa aaatgcagaa gactatggct ttcctggaag aggaacttcc tataactgct
     3061 tgtggcgtat acacatatgg aaagcctgtt cccggtctgg tgacattgag agtgtgcaga
     3121 aaatattcac gataccgctc cacctgccat aaccaaaact caatgagtat ctgtgaagaa
     3181 ttcagccaac aggcagatga taaaggatgt ttcagacaag ttgtaaaaac caaagtattc
     3241 cagctcagac aaaaaggcca cgacatgaag atagaggtgg aagccaaaat caaagaggaa
     3301 ggaacaggaa tagaactaac tggtattgga tcatgtgaaa tagcaaatgc cttaagcaaa
     3361 ctgaaattta ctaaagtaaa tacaaattac aggcctgggc tacctttctc tggacaggtt
     3421 cttcttgttg atgagaaggg taaaccaatc cccaacaaaa acataacttc cgtcgtgtct
     3481 ccacttgggt acctgtccat ttttactacg gatgagcatg gcttggctaa catttccatt
     3541 gacacttcca acttcacagc tccgtttttg agagttgtgg tcacctacaa gcagaaccat
     3601 gtctgctatg ataactggtg gcttgatgaa tttcacacac aggcagacca ttctgcaact
     3661 ctagtctttt ctccaagcca gagttatatt caacttgaac ttgtttttgg tactttggcc
     3721 tgtgggcaaa ctcaggagat tcggatacac tacctcttga atgaagatat catgaagaat
     3781 gaaaaagact taacctttta ctatctgatc aaagcaaggg gaagcatctt caacttagga
     3841 agccatgtgt tgtcgcttga acaaggaaac atgaaaggag tcttttccct cccaattcaa
     3901 gtggagccag gcatggctcc cgaggctcag ctgctcattt atgctatttt acctaatgaa
     3961 gaacttgtcg ctgatgctca gaattttgaa atcgagaagt gttttgccaa caaggtaaat
     4021 ttgagtttcc catcagcaca gagcctgcca gcctctgaca cccacctgaa ggtcaaagcc
     4081 gcgcctctgt ccctctgtgc cctcactgca gtagaccaga gtgtgctgct actgaagccc
     4141 gaagccaagc tctcacctca gtcaatctac aatttgctgc caggaaagac tgtccagggt
     4201 gccttctttg gtgtcccagt gtacaaagac catgagaact gcatcagcgg agaagacatc
     4261 actcacaatg ggatcgtgta cacgccaaag cactctctgg gcgataatga tgcacacagc
     4321 atttttcagt ctgtaggaat aaatattttt accaactcca aaatccacaa accacgcttt
     4381 tgtcaagagt ttcaacacta tccggcaatg ggaggagtgg cacctcaagc cttagctgtg
     4441 gctgcttcag gcccaggatc cagcttcaga gcaatgggcg tgccgatgat ggggttggac
     4501 tactctgatg aaattaatca ggtggtggaa gtaagagaga cagtgcggaa gtacttccct
     4561 gagacctgga tctgggacct ggtgccactg gacgtatccg gggacggtga attggcggta
     4621 aaggtccccg acaccatcac tgagtggaag gccagtgcat tctgcttgtc tggaaccact
     4681 ggccttggcc tctcctccac catctccctt caagccttcc agcccttctt cttggagctc
     4741 actctcccat actctgtggt gcgaggggaa gcctttaccc tcaaagccac cgtgctcaat
     4801 tacatgtctc actgcattca gatccgagtg gacctagaga tttctcctga tttcctggca
     4861 gtcccagtgg ggggccatga aaactctcat tgcatctgtg gaaatgaaag gaaaaccgtg
     4921 tcctgggctg tgaccccaaa gtcgctgggg gaggtgaact tcacagctac cgcagaagcc
     4981 ttgcagtctc cagaattgtg tggcaataag ttgacagaag tgccagccct tgtacacaag
     5041 gacactgtgg tgaagtccgt aatagttgag cctgaaggaa ttgagaagga gcaaacgtac
     5101 aacacactgt tatgcccaca agatactgag ttacaagata attggtcact ggagcttcca
     5161 cccaatgtgg ttgaaggatc tgccagggct acacattccg ttttgggtga tatactgggc
     5221 tctgcaatgc aaaacctcca gaatcttctc cagatgccct atggctgtgg ggaacaaaac
     5281 atggtccttt ttgtccctaa catctatgtt ctgaactatc tgaacgagac acagcagctg
     5341 acagaggcga tcaagtccaa agccattaac tacctcatca gtgggtacca gaggcagctg
     5401 aactatcagc acagtgatgg ctcgtacagc acattcggga accatggtgg tggcaacact
     5461 ccgggaaata cttggctcac tgcgtttgtg ctcaaggcct ttgctcaagc tcagtcgcac
     5521 atctttatag agaagacaca catcacaaat gctttcaact ggctctccat gaaacaaaag
     5581 gagaatggct gtttccaaca gtctggatat ctgcttaaca atgcgatgaa gggtggtgtg
     5641 gatgatgaag tgactctctc tgcctacatc accattgctc tgctggagat gcctctgcct
     5701 gtcacgcaca gtgctgtgcg aaatgctcta ttttgcctgg aaacggcctg ggcctccatc
     5761 tcacagagcc aggaaagtca tgtctatacc aaggcattgc tggcctatgc cttcgccctg
     5821 gcaggaaaca aggccaagag aagcgaactg cttgaatccc taaacaaaga tgctgtgaag
     5881 gaagaggatt cgctgcactg gcaacgccct ggggatgttc agaaagtgaa ggccttatct
     5941 ttctatcaac ctcgggcccc ttctgccgaa gtggagatga cagcttacgt gcttctcgcc
     6001 tatctaacct ctgagtcttc ccggcccaca cgggacctgt cttcatcaga cctgtccaca
     6061 gcatccaaga ttgtgaagtg gatcagcaag caacaaaact cccacggggg cttctcctcc
     6121 actcaggata cagtggtggc tctccaagcc ctctccaaat atggagctgc cacttttacg
     6181 agaagtcaga aagaagtgtt ggtcaccatc gagtcttcag ggaccttctc taagactttc
     6241 catgttaaca gtggcaatcg cctgctgctg caggaagtca ggctgccaga tctgcccgga
     6301 aattacgtca ccaaagggtc aggatcagga tgtgtgtacc ttcagacatc tctaaagtac
     6361 aacatcctcc cagtggcaga tggaaaagca cccttcgctc tgcaagtcaa cactctcccc
     6421 ctaaactttg acaaggcagg agatcacaga acattccaga ttcgcatcaa cgtaagctac
     6481 actggagaac gacccagctc caacatggtc attgttgatg tgaagatggt atcaggcttc
     6541 atacctatga agccatccgt gaaaaagctc caagaccagc ctaacattca gaggactgaa
     6601 gtgaacacca accatgttct aatctacatt gaaaagctaa ccaatcaaac cctcggtttc
     6661 tccttcgcgg tggaacaaga catcccagta aagaacttaa aaccagcccc cataaaagtc
     6721 tatgattatt atgagacaga tgaattcacc gttgaagaat acagtgcccc tttcagtgat
     6781 ggctctgaac aaggaaatgc tggatccgac tacaaagacc atgacggtga ttataaagat
     6841 catgacatcg actacaagga tgacgatgac aagtagcggc cgcgaaggat ctgcgatcgc
     6901 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     6961 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     7021 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     7081 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga
     7141 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg
     7201 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag
     7261 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct
     7321 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt
     7381 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc
     7441 accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc
     7501 accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc
     7561 cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg
     7621 agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac
     7681 cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag
     7741 gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc
     7801 gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc
     7861 atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc
     7921 agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc
     7981 cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc
     8041 gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag
     8101 cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc
     8161 gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta
     8221 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc
     8281 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     8341 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     8401 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     8461 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     8521 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     8581 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     8641 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     8701 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     8761 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     8821 acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt
     8881 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     8941 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     9001 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     9061 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     9121 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     9181 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     9241 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     9301 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     9361 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     9421 tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt
     9481 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     9541 aagataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     9601 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     9661 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     9721 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     9781 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     9841 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9901 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     9961 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
    10021 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
    10081 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt
    10141 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    10201 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    10261 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    10321 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    10381 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    10441 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    10501 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    10561 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    10621 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    10681 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    10741 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    10801 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    10861 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    10921 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    10981 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    11041 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    11101 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    11161 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    11221 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    11281 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    11341 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    11401 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    11461 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    11521 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    11581 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    11641 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    11701 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    11761 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    11821 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    11881 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    11941 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    12001 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    12061 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    12121 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    12181 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    12241 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    12301 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    12361 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    12421 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    12481 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
    12541 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
    12601 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    12661 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
    12721 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//
