LOCUS V037538 8649 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V037538 VERSION V037538 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2303..2926 /label="RAB6B(NM_016577)" /note="RAB6B(NM_016577)" /gene="RAB6B" CDS 2933..2998 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 3042..3253 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3266..3534 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 3569..4276 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 4286..4339 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 4340..4738 /codon_start=1 /gene="Aspergillus terreus BSD" /note="confers resistance to blasticidin" /product="blasticidin S deaminase" /transl_table=1 /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG*" /label="BSD" misc_feature 4745..5332 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5406..5639 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 5711..5832 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 5851..5986 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6024..6040) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6048..6064 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6072..6102) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6117..6138 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6426..7014) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7185..8045) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8046..8150) /gene="bla" /label="AmpR promoter" primer_bind 8624..8640 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgtccgc agggggagat tttgggaatc cactgagaaa 2341 attcaagttg gtgttcttgg gggagcagag cgtcgggaag acgtctctga ttacgaggtt 2401 catgtacgac agcttcgaca acacatacca ggcaaccatt gggattgact tcttgtcaaa 2461 aaccatgtac ttggaggacc gcacggtgcg actgcagctc tgggacacag ctggtcagga 2521 gaggttccgc agcctgatcc ccagctacat ccgggactcc acggtggctg tggtggtgta 2581 cgacatcaca aatctcaact ccttccaaca gacctctaag tggatcgacg acgtcaggac 2641 agagaggggc agtgatgtta tcatcatgct ggtgggcaac aagacggacc tggctgataa 2701 gaggcagata accatcgagg agggggagca gcgcgccaaa gaactgagcg tcatgttcat 2761 tgagaccagt gcgaagactg gctacaacgt gaagcagctt tttcgacgtg tggcgtcggc 2821 tctacccgga atggagaatg tccaggagaa aagcaaagaa gggatgatcg acatcaagct 2881 ggacaaaccc caggagcccc cggccagcga gggcggctgc tcctgcggat ccgactacaa 2941 agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta 3001 gcggccgcga aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc 3061 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg 3121 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg 3181 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc 3241 cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc 3301 tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc 3361 ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg 3421 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct 3481 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct 3541 gtgaccggcg cctacgctag acgccaccat ggtgagcaag ggcgaggagg ataacatggc 3601 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga 3661 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct 3721 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat 3781 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc 3841 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac 3901 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg 3961 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc 4021 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa 4081 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc 4141 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga 4201 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat 4261 ggacgagctg tacaagggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt 4321 ggaggagaat cccggcccta tggccaagcc tttgtctcaa gaagaatcca ccctcattga 4381 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc 4441 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg 4501 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac 4561 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg 4621 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca 4681 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaagt 4741 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 4801 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 4861 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 4921 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 4981 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 5041 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 5101 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 5161 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 5221 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 5281 tctccgcctt cgccctcaga cgagtcggat ctccctttgg ccgcctcccc gcctggtacc 5341 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 5401 gggactggaa gggctaattc actcccaacg aagataagat ctgctttttg cttgtactgg 5461 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 5521 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 5581 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 5641 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 5701 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 5761 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 5821 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc 5881 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 5941 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga 6001 gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 6061 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 6121 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 6181 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 6241 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 6301 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 6361 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 6421 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 6481 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 6541 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 6601 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 6661 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 6721 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 6781 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 6841 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 6901 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 6961 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 7021 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 7081 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 7141 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 7201 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 7261 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 7321 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 7381 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 7441 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 7501 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 7561 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 7621 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 7681 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 7741 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 7801 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 7861 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 7921 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 7981 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 8041 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 8101 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 8161 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 8221 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga 8281 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa 8341 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca 8401 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta 8461 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg 8521 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg 8581 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt 8641 gccaagctg //