LOCUS       V033077                11383 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033077
VERSION     V033077
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..6237
                     /label="RAD50(NM_005732)"
                     /note="RAD50(NM_005732)"
                     /gene="RAD50"
     CDS             6244..6309
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6353..6564
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6577..6845
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6872..7471
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7478..8066
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8140..8373
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8445..8566
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8585..8720
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8758..8774)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8782..8798
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8806..8836)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8851..8872
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9160..9748)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9919..10779)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10780..10884)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11358..11374
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgtcccgg atcgaaaaga tgagcattct gggcgtgcgg
     2341 agttttggaa tagaggacaa agataagcaa attatcactt tcttcagccc ccttacaatt
     2401 ttggttggac ccaatggggc gggaaagacg accatcattg aatgtctaaa atatatttgt
     2461 actggagatt tccctcctgg aaccaaagga aatacatttg tacacgatcc caaggttgct
     2521 caagaaacag atgtgagagc ccagattcgt ctgcaatttc gtgatgtcaa tggagaactt
     2581 atagctgtgc aaagatctat ggtgtgtact cagaaaagca aaaagacaga atttaaaact
     2641 ctggaaggag tcattactag aacaaagcat ggtgaaaagg tcagtctgag ctctaagtgt
     2701 gcagaaattg accgagaaat gatcagttct cttggggttt ccaaggctgt gctaaataat
     2761 gtcattttct gtcatcaaga agattctaat tggcctttaa gtgaaggaaa ggctttgaag
     2821 caaaagtttg atgagatttt ttcagcaaca agatacatta aagccttaga aacacttcgg
     2881 caggtacgtc agacacaagg tcagaaagta aaagaatatc aaatggaact aaaatatctg
     2941 aagcaatata aggaaaaagc ttgtgagatt cgtgatcaga ttacaagtaa ggaagcccag
     3001 ttaacatctt caaaggaaat tgtcaaatcc tatgagaatg aacttgatcc attgaagaat
     3061 cgtctaaaag aaattgaaca taatctctct aaaataatga aacttgacaa tgaaattaaa
     3121 gccttggata gccgaaagaa gcaaatggag aaagataata gtgaactgga agagaaaatg
     3181 gaaaaggttt ttcaagggac tgatgagcaa ctaaatgact tatatcacaa tcaccagaga
     3241 acagtaaggg agaaagaaag gaaattggta gactgtcatc gtgaactgga aaaactaaat
     3301 aaagaatcta ggcttctcaa tcaggaaaaa tcagaactgc ttgttgaaca gggtcgtcta
     3361 cagctgcaag cagatcgcca tcaagaacat atccgagcta gagattcatt aattcagtct
     3421 ttggcaacac agctagaatt ggatggcttt gagcgtggac cattcagtga aagacagatt
     3481 aaaaattttc acaaacttgt gagagagaga caagaagggg aagcaaaaac tgccaaccaa
     3541 ctgatgaatg actttgcaga aaaagagact ctgaaacaaa aacagataga tgagataaga
     3601 gataagaaaa ctggactggg aagaataatt gagttaaaat cagaaatcct aagtaagaag
     3661 cagaatgagc tgaaaaatgt gaagtatgaa ttacagcagt tggaaggatc ttcagacagg
     3721 attcttgaac tggaccagga gctcataaaa gctgaacgtg agttaagcaa ggctgagaaa
     3781 aacagcaatg tagaaacctt aaaaatggaa gtaataagtc tccaaaatga aaaagcagac
     3841 ttagacagga ccctgcgtaa acttgaccag gagatggagc agttaaacca tcatacaaca
     3901 acacgtaccc aaatggagat gctgaccaaa gacaaagctg acaaagatga acaaatcaga
     3961 aaaataaaat ctaggcacag tgatgaatta acctcactgt tgggatattt tcccaacaaa
     4021 aaacagcttg aagactggct acatagtaaa tcaaaagaaa ttaatcagac cagggacaga
     4081 cttgccaaat tgaacaagga actagcttca tctgagcaga ataaaaatca tataaataat
     4141 gaactaaaaa gaaaggaaga gcagttgtcc agttacgaag acaagctgtt tgatgtttgt
     4201 ggtagccagg attttgaaag tgatttagac aggcttaaag aggaaattga aaaatcatca
     4261 aaacagcgag ccatgctggc tggagccaca gcagtttact cccagttcat tactcagcta
     4321 acagacgaaa accagtcatg ttgccccgtt tgtcagagag tttttcagac agaggctgag
     4381 ttacaagaag tcatcagtga tttgcagtct aaactgcgac ttgctccaga taaactcaag
     4441 tcaacagaat cagagctaaa aaaaaaggaa aagcggcgtg atgaaatgct gggacttgtg
     4501 cccatgaggc aaagcataat tgatttgaag gagaaggaaa taccagaatt aagaaacaaa
     4561 ctgcagaatg tcaatagaga catacagcgc ctaaagaacg acatagaaga acaagaaaca
     4621 ctcttgggta caataatgcc tgaagaagaa agtgccaaag tatgcctgac agatgttaca
     4681 attatggaga ggttccagat ggaacttaaa gatgttgaaa gaaaaattgc acaacaagca
     4741 gctaagctac aaggaataga cttagatcga actgtccaac aagtcaacca ggagaaacaa
     4801 gagaaacagc acaagttaga cacagtttct agtaagattg aattgaatcg taagcttata
     4861 caggaccagc aggaacagat tcaacatcta aaaagtacaa caaatgagct aaaatctgag
     4921 aaacttcaga tatccactaa tttgcaacgt cgtcagcaac tggaggagca gactgtggaa
     4981 ttatccactg aagttcagtc tttgtacaga gagataaagg atgctaaaga gcaggtaagc
     5041 cctttggaaa caacattgga aaagttccag caagaaaaag aagaattaat caacaaaaaa
     5101 aatacaagca acaaaatagc acaggataaa ctgaatgata ttaaagagaa ggttaaaaat
     5161 attcatggct atatgaaaga cattgagaat tatattcaag atgggaaaga cgactataag
     5221 aagcaaaaag aaactgaact taataaagta atagctcaac taagtgaatg cgagaaacac
     5281 aaagaaaaga taaatgaaga tatgagactc atgagacaag atattgatac acagaagata
     5341 caagaaaggt ggctacaaga taaccttact ttaagaaaaa gaaatgagga actaaaagaa
     5401 gttgaagaag aaagaaaaca acatttgaag gaaatgggtc aaatgcaggt tttgcaaatg
     5461 aaaagtgaac atcagaagtt ggaagagaac atagacaata taaaaagaaa tcataatttg
     5521 gcattagggc gacagaaagg ttatgaagaa gaaattattc attttaagaa agaacttcga
     5581 gaaccacaat ttcgggatgc tgaggaaaag tatagagaaa tgatgattgt tatgaggaca
     5641 acagaacttg tgaacaagga tctggatatt tattataaga ctcttgacca agcaataatg
     5701 aaatttcaca gtatgaaaat ggaagaaatc aataaaatta tacgtgacct gtggcgaagt
     5761 acctatcgtg gacaagatat tgaatacata gaaatacggt ctgatgccga tgaaaatgta
     5821 tcagcttctg ataaaaggcg gaattataac taccgagtgg tgatgctgaa gggagacaca
     5881 gccttggata tgcgaggacg atgcagtgct ggacaaaagg tattagcctc actcatcatt
     5941 cgcctggccc tggctgaaac gttctgcctc aactgtggca tcattgcctt ggatgagcca
     6001 acaacaaatc ttgaccgaga aaacattgaa tctcttgcac atgctctggt tgagataata
     6061 aaaagtcgct cacagcagcg taacttccag cttctggtaa tcactcatga tgaagatttt
     6121 gtggagcttt taggacgttc tgaatatgtg gagaaattct acaggattaa aaagaacatc
     6181 gatcagtgct cagagattgt gaaatgcagt gttagctccc tgggattcaa tgttcatgga
     6241 tccgactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac
     6301 gatgacaagt agcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag
     6361 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     6421 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     6481 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     6541 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     6601 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     6661 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     6721 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     6781 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     6841 agatccaagc tgtgaccggc gcctacgcta gatgaccgag tacaagccca cggtgcgcct
     6901 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta
     6961 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca
     7021 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     7081 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     7141 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     7201 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     7261 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     7321 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     7381 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     7441 ctggtgcatg acccgcaagc ccggtgcctg agtcgacaat caacctctgg attacaaaat
     7501 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     7561 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     7621 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     7681 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     7741 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     7801 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     7861 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     7921 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     7981 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     8041 gatctccctt tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag
     8101 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     8161 aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     8221 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     8281 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8341 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     8401 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     8461 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8521 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     8581 agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8641 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8701 aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt aatcatggtc
     8761 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8821 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8881 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8941 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     9001 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     9061 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     9121 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     9181 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9241 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9301 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9361 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9421 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9481 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9541 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9601 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9661 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9721 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9781 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9841 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9901 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9961 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    10021 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    10081 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    10141 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    10201 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    10261 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    10321 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    10381 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10441 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10501 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10561 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10621 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10681 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10741 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10801 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10861 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    10921 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    10981 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    11041 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    11101 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    11161 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
    11221 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    11281 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    11341 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
