LOCUS       V043750                11431 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043750
VERSION     V043750
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5457
                     /label="RASA1(NM_002890)"
                     /note="RASA1(NM_002890)"
                     /gene="RASA1"
     CDS             5464..5529
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5573..5784
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5797..6065
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6094..6103
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6124..6786
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6856..6909
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6910..7509
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7510..8098
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8172..8405
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8477..8598
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8638..8773
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8806..8822)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8830..8846
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8854..8884)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8899..8920
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9208..9796)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9967..10827)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10828..10932)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11406..11422
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga tggcggccga ggccggcagt
     2341 gaggagggcg gcccggtaac agccggagct ggaggaggcg gcgcggcagc gggctccagt
     2401 gcctatcccg cagtgtgtcg ggtgaagata cccgcggccc tgcctgtggc agccgccccc
     2461 tatcctgggc tggtggagac cggagtggct ggaactctgg gtggcggagc cgctttgggg
     2521 tcagagttcc taggagccgg gtctgtggca ggggcactgg ggggagctgg actgacaggg
     2581 ggaggtactg ctgctggcgt agctggtgct gctgctggcg tggccggtgc tgctgttgct
     2641 ggacctagtg gagacatggc tctcaccaaa ctgcccactt cgttgcttgc tgagactctc
     2701 gggccaggcg gcggttttcc ccctctgccc cctccccctt acctgccccc tttgggggcg
     2761 ggcctcggga cagtggacga aggtgactct ctggatggac cagaatacga ggaggaagag
     2821 gtggccatac cgttgaccgc tcctccaact aaccagtggt atcacggaaa acttgacaga
     2881 acgatagcag aagaacgcct caggcaggca gggaagtctg gcagttatct tataagagag
     2941 agtgatcgga ggccagggtc ctttgtactt tcatttctta gccagatgaa tgttgtcaac
     3001 cattttagga ttattgctat gtgtggagat tactacattg gtggaagacg tttttcttca
     3061 ctgtcagacc taataggtta ttacagtcat gtttcttgtt tgcttaaagg agaaaaatta
     3121 ctttacccag ttgcaccacc agagccagta gaagatagaa ggcgtgtacg agctattcta
     3181 ccttacacaa aagtaccaga cactgatgaa ataagtttct taaaaggaga tatgttcatt
     3241 gttcataatg aattagaaga tggatggatg tgggttacaa atttaagaac agatgaacaa
     3301 ggccttattg ttgaagacct agtagaagag gtgggccggg aagaagatcc acatgaagga
     3361 aaaatatggt tccatgggaa gatttccaaa caggaagctt ataatttact aatgacagtt
     3421 ggtcaagtct gcagttttct tgtgaggccc tcagataata ctcctggcga ttattcactt
     3481 tatttccgga ccaatgaaaa tattcagcga tttaaaatat gtccaacgcc aaacaatcag
     3541 tttatgatgg gaggccggta ttataacagc attggggaca tcatagatca ctatcgaaaa
     3601 gaacagattg ttgaaggata ttatcttaag gaacctgtac caatgcagga tcaagaacaa
     3661 gtactcaatg acacagtgga tggcaaggaa atctataata ccatccgtcg taaaacaaag
     3721 gatgcctttt ataaaaacat tgttaagaaa ggttatcttc tgaaaaaggg caaaggaaaa
     3781 cgttggaaaa atttatattt tatcttagag ggtagtgatg cccaacttat ttattttgaa
     3841 agcgaaaaac gagctaccaa accaaaagga ttaatagatc tcagtgtatg ttctgtctat
     3901 gtcgttcatg atagtctctt tggcaggcca aactgttttc agatagtagt tcagcacttt
     3961 agtgaagaac attacatctt ttactttgca ggagaaactc cagaacaagc agaggattgg
     4021 atgaaaggtc tgcaggcatt ttgcaattta cggaaaagta gtccagggac atccaataaa
     4081 cgccttcgtc aggtcagcag ccttgtttta catattgaag aagcccataa actcccagta
     4141 aaacatttta ctaatccata ttgtaacatc tacctgaata gtgtccaagt agcaaaaact
     4201 catgcaaggg aagggcaaaa cccagtatgg tcagaagagt ttgtctttga tgatcttcct
     4261 cctgacatca atagatttga aataactctt agtaataaaa caaagaaaag caaagatcct
     4321 gatatcttat ttatgcgctg ccagttgagc cgattacaga aagggcatgc cacagatgaa
     4381 tggtttctgc tcagctccca tataccatta aaaggtattg aaccagggtc cctgcgtgtt
     4441 cgagcacgat actctatgga aaaaatcatg ccagaagaag agtacagtga atttaaagag
     4501 cttatactgc aaaaggaact tcatgtagtc tatgctttat cacatgtatg tggacaagac
     4561 cgaacactac tggccagcat cctactgagg atttttcttc acgaaaagct tgaatcgttg
     4621 ttgttatgca cactaaatga cagagaaata agcatggaag atgaagccac taccctattt
     4681 cgagccacaa cacttgcaag caccttgatg gagcagtata tgaaagccac tgctacacag
     4741 tttgttcatc atgctttgaa agactctatt ttaaagataa tggaaagcaa gcagtcttgt
     4801 gagttaagtc catcaaagtt agaaaaaaat gaagatgtga acactaattt aacacaccta
     4861 ttgaacatac tttcagagct tgtggagaaa atattcatgg cttcagaaat acttccaccg
     4921 acattgagat atatttatgg gtgtttacag aaatctgttc agcataagtg gcctacaaat
     4981 accaccatga gaacaagagt tgttagtggt tttgtttttc ttcgactcat ctgtcctgcc
     5041 atcctgaatc cacggatgtt caatatcatc tcagattctc catctcctat tgctgcaaga
     5101 acactgatat tagtggctaa atctgtgcag aacttagcaa atcttgtgga atttggagct
     5161 aaggagccct acatggaagg tgtcaatcca ttcatcaaaa gcaacaaaca tcgtatgatc
     5221 atgtttttag atgaacttgg gaatgtacct gaacttccgg acactacaga gcattctaga
     5281 acggacctgt cccgtgattt agcagcattg catgagattt gcgtggctca ttcagatgaa
     5341 cttcgaacgc tcagtaatga gcgtggtgca cagcagcacg tattgaaaaa gcttctggct
     5401 ataacagaac tgcttcaaca aaaacaaaac cagtatacaa aaaccaatga tgtcagggga
     5461 tccgactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac
     5521 gatgacaagt agcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag
     5581 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     5641 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     5701 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     5761 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     5821 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     5881 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     5941 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     6001 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     6061 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc
     6121 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag
     6181 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc
     6241 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc
     6301 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac
     6361 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc
     6421 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc
     6481 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag
     6541 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg
     6601 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc
     6661 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg
     6721 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc
     6781 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc
     6841 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
     6901 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     6961 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     7021 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     7081 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     7141 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     7201 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     7261 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     7321 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     7381 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     7441 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     7501 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     7561 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     7621 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     7681 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     7741 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     7801 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     7861 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     7921 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7981 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     8041 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     8101 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     8161 aaagggggga ctggaagggc taattcactc ccaacgaaga taagatctgc tttttgcttg
     8221 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     8281 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     8341 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8401 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     8461 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     8521 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8581 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     8641 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8701 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     8761 ttttttggag gcctagactt ttgcagagac caaattcgta atcatgtcat agctgtttcc
     8821 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8881 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8941 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     9001 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     9061 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     9121 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     9181 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     9241 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     9301 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     9361 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     9421 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9481 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     9541 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     9601 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     9661 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9721 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9781 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9841 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9901 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9961 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    10021 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    10081 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    10141 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    10201 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    10261 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    10321 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    10381 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    10441 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    10501 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    10561 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    10621 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    10681 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    10741 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10801 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10861 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10921 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
    10981 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
    11041 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
    11101 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
    11161 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
    11221 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
    11281 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    11341 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
    11401 acgttgtaaa acgacggcca gtgccaagct g
//
