LOCUS       V041089                10400 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041089
VERSION     V041089
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5232
                     /label="RRBP1(NM_001042576)"
                     /note="RRBP1(NM_001042576)"
                     /gene="RRBP1"
     CDS             5239..5265
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5269..5295
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5299..5325
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5370..5581
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5594..5862
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5889..6488
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6495..7083
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7157..7390
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7462..7583
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7602..7737
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7775..7791)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7799..7815
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7823..7853)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7868..7889
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8177..8765)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8936..9796)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9797..9901)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10375..10391
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggatatt tacgacactc aaaccttggg ggttgtggtc
     2341 tttggaggat tcatggttgt ttctgccatt ggcatcttcc tggtgtcgac tttctccatg
     2401 aaggaaacgt catatgaaga agccctagcc aaccagcgca aggagatggc gaaaactcac
     2461 caccagaaag tcgagaagaa aaagaaggag aaaacagtgg agaagaaagg aaagaccaag
     2521 aaaaaggaag agaaacctaa tgggaagata cctgatcatg atccagcccc caatgtgact
     2581 gtcctccttc gagaaccagt gcgggctcct gctgtggctg tggctccaac cccagtgcag
     2641 ccccccatta tcgttgctcc tgtcgccaca gttccagcca tgccccagga gaagctggcc
     2701 tcctccccca aggacaaaaa gaagaaggag aaaaaagtgg caaaagtgga accagctgtc
     2761 agctctgtag tgaattccat ccaggttctc acttcgaagg ctgccatctt ggaaactgct
     2821 cccaaggagg gcagaaatac agatgtggcc cagagcccag aggcaccaaa gcaagaggct
     2881 cctgccaaga agaagtctgg ttcaaagaaa aaagggcccc cagatgccga cggccctctc
     2941 tacctcccct acaagacgct ggtctccacg gttgggagca tggtgttcaa cgagggcgag
     3001 gcccagcggc tcatcgagat cctgtctgag aaggctggca tcattcagga cacctggcac
     3061 aaggccactc agaagggtga ccctgtggcg attctgaaac gccagctgga agagaaggaa
     3121 aaactgctgg ccacagaaca ggaagatgcg gctgtcgcca agagcaaact gagggagctc
     3181 aacaaggaga tggcagcaga aaaggccaaa gcagcagccg gggaggccaa agtgaaaaag
     3241 cagctggtgg cccgggagca ggagatcacg gctgtgcagg cacgcatgca ggccagctac
     3301 cgggagcacg tgaaggaggt gcagcagctg cagggcaaga tccggactct tcaggagcag
     3361 ctggagaatg gccccaacac gcagctggcc cgcctgcagc aggagaactc catcctgcgg
     3421 gatgccttga accaggccac gagccaggtg gagagcaagc agaacgcaga gctggccaag
     3481 cttcggcagg agctcagcaa ggtcagcaaa gagctggtgg agaagtcaga ggctgtgcgg
     3541 caagatgagc agcagcggaa agctctggaa gccaaggcag ctgccttcga gaagcaggtc
     3601 ctgcagctgc aggcgtccca cagggagagt gaggaggccc tgcagaagcg cctggacgag
     3661 gtcagccggg agctgtgcca cacgcagagc agccacgcca gcctccgggc ggatgccgag
     3721 aaggcccagg agcaacagca gcagatggcc gagctgcaca gcaagttaca gtcctccgag
     3781 gcggaggtgc gcagcaaatg cgaggagctg agtggcctcc acgggcagct ccaggaggcc
     3841 agggcggaga actcccagct cacagagaga atccgttcca ttgaggccct gctggaggcg
     3901 ggccaggcgc gggatgccca ggacgtccag gccagccagg cggaggctga ccagcagcag
     3961 actcgcctca aggagctgga gtcccaggtg tcgggtctgg agaaggaggc catcgagctc
     4021 agggaggccg tcgagcagca gaaagtgaag aacaatgacc tccgggagaa gaactggaag
     4081 gccatggagg cactggccac ggccgagcag gcctgcaagg agaagctgct ctccctgacc
     4141 caggccaagg aggaatcgga gaagcagctc tgtctgattg aggcgcagac catggaggcc
     4201 ctgctggctc tgctcccaga actctctgtc ttggcacaac agaattacac cgagtggctg
     4261 caggatctca aagagaaagg ccccacgctg ctgaagcacc cgccagctcc cgcggagccc
     4321 tcctcggacc tggcctccaa gttgagggag gccgaggaga cgcagagcac actgcaggcc
     4381 gagtgtgacc agtaccgcag catcctggcg gagacggagg gcatgctcag agacctgcag
     4441 aagagcgtgg aggaggagga gcaggtgtgg agggccaagg tgggcgccgc agaggaggag
     4501 ctccagaagt cccgggtcac agtgaagcat ctcgaagaga ttgtagagaa gctaaaagga
     4561 gaacttgaaa gttcggacca ggtgagggag cacacgtcgc atttggaggc agagctggaa
     4621 aagcacatgg cggccgccag cgccgagtgc cagaactacg ccaaggaggt ggcagggctg
     4681 aggcaacttc tcctagaatc tcaatctcag ctcgatgccg ccaagagcga agcccagaaa
     4741 cagagcgatg agcttgccct ggtcaggcag cagttgagtg aaatgaagag ccacgtagag
     4801 gatggtgaca tagctggggc cccagcttcc tccccagagg cgcccccagc cgagcaggac
     4861 cccgttcagc tgaagacgca gctggagtgg acagaagcca tcctggagga tgagcagaca
     4921 cagcggcaga agctcacggc cgagtttgag gaggctcaga cctcggcatg tcggttacaa
     4981 gaagaattgg agaagctccg cacagccggc cccctagagt cttcagaaac agaggaggcc
     5041 tcacagctga aggagagact agaaaaagag aagaagttaa caagtgacct ggggcgcgcc
     5101 gccacgagac tgcaggagct tctgaagacg acccaggagc agctggcaag ggagaaggac
     5161 acggtgaaga agctgcagga acagctggaa aaggcagagg acggcagcag ctcaaaggag
     5221 ggcacctctg tcggatccta cccatacgac gtacctgatt atgcaactta tccttatgat
     5281 gtacctgatt atgctagcta tccttatgat gtcccagact acgcataggc ggccgcgaag
     5341 gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     5401 gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa
     5461 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     5521 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     5581 gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg
     5641 ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc
     5701 gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc
     5761 ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact
     5821 ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc
     5881 tacgctagat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
     5941 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     6001 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     6061 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     6121 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     6181 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     6241 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     6301 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     6361 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     6421 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     6481 gtgcctgagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6541 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6601 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6661 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6721 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6781 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6841 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     6901 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6961 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     7021 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     7081 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7141 aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt
     7201 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7261 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7321 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7381 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     7441 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     7501 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7561 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     7621 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7681 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7741 acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     7801 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7861 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7921 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7981 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8041 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8101 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8161 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8221 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8281 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8341 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8401 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8461 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8521 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8581 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     8641 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8701 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8761 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8821 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8881 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8941 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9001 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9061 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9121 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9181 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9241 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9301 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9361 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9421 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9481 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9541 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9601 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9661 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9721 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9781 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9841 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9901 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     9961 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
    10021 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
    10081 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
    10141 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
    10201 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
    10261 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
    10321 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
    10381 cgacggccag tgccaagctg
//
