LOCUS       V028235                10660 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028235
VERSION     V028235
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4692
                     /label="RRM1(NM_001033)"
                     /note="RRM1(NM_001033)"
                     /gene="RRM1"
     CDS             4693..4758
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4802..5013
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5026..5294
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5323..5332
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5353..6015
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6085..6138
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6139..6738
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6739..7327
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7401..7634
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7706..7827
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7867..8002
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8035..8051)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8059..8075
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8083..8113)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8128..8149
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8437..9025)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9196..10056)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10057..10161)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10635..10651
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc atgtgatcaa gcgagatggc
     2341 cgccaagaac gagtcatgtt tgacaaaatt acatctcgaa tccagaagct ttgttatgga
     2401 ctcaatatgg attttgttga tcctgctcag atcaccatga aagtaatcca aggcttgtac
     2461 agtggggtca ccacagtgga actagatact ttggctgctg aaacagctgc aaccttgact
     2521 actaagcacc ctgactatgc tatcctggca gccaggatcg ctgtctctaa cttgcacaaa
     2581 gaaacaaaga aagtgttcag tgatgtgatg gaagacctct ataactacat aaatccacat
     2641 aatggcaaac actctcccat ggtggccaag tcaacattgg atattgttct ggccaataaa
     2701 gatcgcctga attctgctat tatctatgac cgagatttct cttacaatta cttcggcttt
     2761 aagacgctag agcggtctta tttgttgaag atcaatggaa aagtggctga aagaccacaa
     2821 catatgttga tgagagtatc tgttgggatc cacaaagaag acattgatgc agcaattgaa
     2881 acatataatc ttctttctga gaggtggttt actcatgctt cgcccactct cttcaatgct
     2941 ggtaccaacc gcccacaact ttctagctgt tttcttctga gtatgaaaga tgacagcatt
     3001 gaaggcattt atgacactct aaagcaatgt gcattgattt ctaagtctgc tggaggaatt
     3061 ggtgttgctg tgagttgtat tcgggctact ggcagctaca ttgctgggac taatggcaat
     3121 tccaatggcc ttgtaccgat gctgagagta tataacaaca cagctcgata tgtggatcaa
     3181 ggtgggaaca agcgtcctgg ggcatttgct atttacctgg agccttggca tttagacatc
     3241 tttgaattcc ttgatttaaa gaagaacaca ggaaaggaag agcagcgtgc cagagatctt
     3301 ttctttgctc tttggattcc ggatctcttc atgaaacgag tggagactaa tcaggactgg
     3361 tctttgatgt gtccaaatga gtgtcctggt ctggatgagg tttggggaga ggaatttgag
     3421 aaactatatg caagttatga gaaacaaggt cgtgtccgca aagttgtaaa agctcagcag
     3481 ctttggtatg ccatcattga gtctcagacg gaaacaggca ccccgtatat gctctacaaa
     3541 gattcctgta atcgaaagag caaccagcag aacctgggaa ccatcaaatg cagcaacctg
     3601 tgcacagaaa tagtggagta caccagcaaa gatgaggttg ctgtttgtaa tttggcttcc
     3661 ctggccctga atatgtatgt cacatcagaa cacacatacg actttaagaa gttggctgaa
     3721 gtcactaaag tcgttgtccg aaacttgaat aaaattattg atataaacta ctatcctgta
     3781 ccagaggcat gcctatcaaa taaacgccat cgccccattg gaattggggt acaaggtctg
     3841 gcagatgctt ttatcctgat gagataccct tttgagagtg cagaagccca gttactgaat
     3901 aagcagatct ttgaaactat ttattatggt gctctggaag ccagctgtga ccttgccaag
     3961 gagcagggcc catacgaaac ctatgagggc tctccagtta gcaaaggaat tcttcagtat
     4021 gatatgtgga atgttactcc tacagaccta tgggactgga aggttctcaa ggagaagatt
     4081 gcaaagtatg gtataagaaa cagtttactt attgccccga tgcctacagc ttccactgct
     4141 cagatcctgg ggaataatga gtccattgaa ccttacacca gcaacatcta tactcgcaga
     4201 gtcttgtcag gagaatttca gattgtaaat cctcacttat tgaaagatct taccgagcgg
     4261 ggcctatggc atgaagagat gaaaaaccag attattgcat gcaatggctc tattcagagc
     4321 ataccagaaa ttcctgatga cctgaagcaa ctttataaaa ctgtgtggga aatctctcag
     4381 aaaactgttc tcaagatggc agctgagaga ggtgctttca ttgatcaaag ccaatctttg
     4441 aacatccaca ttgctgagcc taactatggc aaactcacta gtatgcactt ctacggctgg
     4501 aagcagggtt tgaagactgg gatgtattat ttaaggacaa gaccagcggc taatccaatc
     4561 cagttcactc taaataagga gaagctaaaa gataaagaaa aggtatcaaa agaggaagaa
     4621 gagaaggaga ggaacacagc agccatggtg tgctctttgg agaatagaga tgaatgtctg
     4681 atgtgtggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     4741 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     4801 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     4861 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     4921 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     4981 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     5041 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     5101 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     5161 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     5221 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     5281 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     5341 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     5401 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     5461 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     5521 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     5581 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     5641 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     5701 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     5761 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     5821 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     5881 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     5941 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     6001 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     6061 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     6121 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6181 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6241 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6301 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6361 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6421 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6481 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6541 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6601 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6661 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6721 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6781 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6841 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6901 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6961 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7021 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7081 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     7141 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7201 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7261 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7321 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7381 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
     7441 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7501 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7561 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7621 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     7681 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     7741 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7801 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     7861 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7921 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     7981 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
     8041 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8101 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8161 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8221 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8281 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8341 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8401 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8461 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8521 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8581 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8641 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8701 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8761 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8821 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     8881 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8941 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9001 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9061 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9121 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9181 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9241 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9301 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9361 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9421 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9481 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9541 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9601 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9661 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9721 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9781 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9841 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9901 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9961 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10021 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10081 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10141 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    10201 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    10261 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    10321 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    10381 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    10441 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    10501 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    10561 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    10621 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
