LOCUS       V031723                10784 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031723
VERSION     V031723
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4878
                     /label="SECISBP2(NM_024077)"
                     /note="SECISBP2(NM_024077)"
                     /gene="SECISBP2"
     promoter        4943..5154
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5167..5435
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5464..5473
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5494..6156
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6226..6279
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6280..6879
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6880..7467
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7541..7774
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7846..7967
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7986..8121
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8159..8175)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8183..8199
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8207..8237)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8252..8273
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8561..9149)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9320..10180)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10181..10285)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10759..10775
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cgtcggaggg gccgcgggag
     2341 cccgaaagcg agggcatcaa gttatcagca gatgtcaaac catttgtccc cagatttgcc
     2401 gggctcaatg tggcatggtt agagtcctca gaagcatgtg tcttccccag ctctgcagcc
     2461 acatactatc cgtttgttca ggaaccacca gtgacagagc agaaaatata tactgaagac
     2521 atggcctttg gagcttcaac ttttccacct cagtatttat cttctgagat aactcttcat
     2581 ccatatgcct attctcctta tacccttgac tccacacaga atgtttactc agtgcctggc
     2641 tcccagtatc tttataacca acccagttgt taccgaggtt ttcaaacagt gaagcatcga
     2701 aatgagaaca catgccctct cccacaagaa atgaaagctc tgtttaagaa gaaaacctat
     2761 gatgagaaaa aaacgtatga tcagcaaaag tttgacagtg aaagggctga tggaactata
     2821 tcatctgaga taaaatcagc tagaggttca catcatttgt ccatttacgc tgagaatagt
     2881 ttgaaatcag atggttacca taagcgaaca gacaggaaat ccagaatcat tgcaaaaaat
     2941 gtatctacct ccaaacctga gtttgaattt accacactgg actttcctga actgcaaggt
     3001 gcagagaaca atatgtcaga gatacagaag caacccaagt ggggacctgt ccactctgtc
     3061 tctaccgaca tttctcttct aagagaagta gtaaaaccag ctgcagtgtt atcaaagggt
     3121 gaaatagtgg tgaaaaataa cccaaatgaa tctgtaactg ctaatgccgc taccaattct
     3181 ccttcatgta caagagagtt atcttggaca ccaatgggtt atgttgttcg acagacatta
     3241 tctacagaac tgtcagcagc ccctaaaaat gttacttcta tgataaactt aaagaccatt
     3301 gcttcatcag cagatcctaa aaatgttagt ataccatctt ctgaagcttt atcttcggat
     3361 ccttcctaca acaaagaaaa acacattatt catcctaccc aaaagtctaa agcatcacaa
     3421 ggtagtgacc ttgaacaaaa tgaagcctca agaaagaata agaaaaagaa agaaaaatct
     3481 acatcaaaat atgaagtcct gacagttcaa gagcctccaa ggattgaaga tgccgaggaa
     3541 tttcccaacc tggcagttgc atctgaaaga agagacagaa tagagacacc gaaatttcaa
     3601 tctaagcagc agccacagga taattttaaa aataatgtaa agaagagcca gcttccagtg
     3661 cagttggact tggggggcat gctgacagcc ctggagaaga agcagcactc tcagcatgca
     3721 aagcagtcct ccaaaccagt ggtagtctca gttggagcag tgccagtcct ttccaaagaa
     3781 tgtgcatcag gggagagagg ccgccgcatg agtcaaatga agaccccgca caatcccttg
     3841 gactccagcg ccccactgat gaagaaaggg aagcagaggg agatccccaa ggccaagaag
     3901 ccaacctcac tgaagaagat tattttgaaa gaacggcaag agagaaagca gcgtctccaa
     3961 gaaaatgctg tgagtccagc ttttaccagt gatgacacac aagatggaga gagtggtggt
     4021 gatgaccagt ttcccgagca ggcagagctg tcagggccag aggggatgga cgaactgatc
     4081 tccactcctt cggttgagga caagtctgaa gagccaccag gcacagagct ccagagggac
     4141 acagaggcct cccaccttgc tcccaatcac accaccttcc ctaagatcca cagccgcaga
     4201 ttcagggatt actgcagcca gatgcttagt aaagaagtgg atgcttgtgt taccgaccta
     4261 ctcaaagaac tggtccgttt ccaagaccgt atgtaccaga aagatccagt caaggccaag
     4321 actaaacgtc gacttgtgtt ggggttgagg gaggttctca aacacctgaa gctcaaaaaa
     4381 ctgaaatgtg tcattatttc tcccaactgt gagaagatac agtcaaaagg tgggctggat
     4441 gacactttgc acacaattat tgattatgcc tgtgagcaga acattccctt tgtgtttgct
     4501 ctcaaccgca aagctctggg gcgcagtttg aataaggcag ttcctgtcag tgtggtgggg
     4561 atcttcagct atgatggggc ccaggatcag ttccacaaga tggttgagct gacagtggcg
     4621 gcccgacagg cgtacaagac catgctggag aatgtgcagc aggagctggt gggagagccc
     4681 aggcctcagg cacctcccag cctacccaca cagggcccca gctgccctgc agaagatggc
     4741 cccccagccc tgaaagaaaa agaagagcca cactacattg aaatctggaa aaaacatctg
     4801 gaagcataca gtggatgtac cctggagcta gaagaatcct tggaggcttc aacctctcaa
     4861 atgatgaatt tgaatttatg aggatccctg cgatatccag cgcggccgcg aaggatctgc
     4921 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     4981 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     5041 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     5101 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     5161 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     5221 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     5281 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     5341 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     5401 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta
     5461 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc
     5521 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag
     5581 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac
     5641 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac
     5701 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag
     5761 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg
     5821 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac
     5881 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg
     5941 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg
     6001 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc
     6061 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag
     6121 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat
     6181 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt
     6241 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg
     6301 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     6361 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     6421 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     6481 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     6541 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     6601 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     6661 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     6721 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     6781 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     6841 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa
     6901 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6961 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7021 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7081 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7141 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7201 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7261 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     7321 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7381 cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt
     7441 cggatctccc tttggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca
     7501 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     7561 caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     7621 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7681 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7741 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     7801 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     7861 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7921 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     7981 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8041 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8101 gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt
     8161 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8221 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8281 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8341 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8401 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8461 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8521 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8581 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8641 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8701 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8761 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8821 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8881 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8941 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9001 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9061 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9121 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9181 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9241 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9301 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9361 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9421 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9481 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9541 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9601 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9661 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9721 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9781 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9841 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9901 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9961 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10021 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10081 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10141 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10201 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10261 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
    10321 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
    10381 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
    10441 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
    10501 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    10561 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
    10621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    10681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    10741 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
