LOCUS V025245 10364 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V025245 VERSION V025245 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..4407 /label="SIGLEC10(NM_033130)" /note="SIGLEC10(NM_033130)" /gene="SIGLEC10" CDS 4414..4479 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4523..4734 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4747..5015 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 5044..5053 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 5074..5736 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 5806..5859 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 5860..6459 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6460..7047 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7121..7354 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7426..7547 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7566..7701 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7739..7755) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7763..7779 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7787..7817) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7832..7853 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8141..8729) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8900..9760) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9761..9865) /gene="bla" /label="AmpR promoter" primer_bind 10339..10355 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc tactgccact gctgctgtcc 2341 tcgctgctgg gcgggtccca ggctatggat gggagattct ggatacgagt gcaggagtca 2401 gtgatggtgc cggagggcct gtgcatctct gtgccctgct ctttctccta cccccgacag 2461 gactggacag ggtctacccc agcttatggc tactggttca aagcagtgac tgagacaacc 2521 aagggtgctc ctgtggccac aaaccaccag agtcgagagg tggaaatgag cacccggggc 2581 cgattccagc tcactgggga tcccgccaag gggaactgct ccttggtgat cagagacgcg 2641 cagatgcagg atgagtcaca gtacttcttt cgggtggaga gaggaagcta tgtgagatat 2701 aatttcatga acgatgggtt ctttctaaaa gtaacagccc tgactcagaa gcctgatgtc 2761 tacatccccg agaccctgga gcccgggcag ccggtgacgg tcatctgtgt gtttaactgg 2821 gcctttgagg aatgtccacc cccttctttc tcctggacgg gggctgccct ctcctcccaa 2881 ggaaccaaac caacgacctc ccacttctca gtgctcagct tcacgcccag accccaggac 2941 cacaacaccg acctcacctg ccatgtggac ttctccagaa agggtgtgag cgcacagagg 3001 accgtccgac tccgtgtggc ctatgccccc agagaccttg ttatcagcat ttcacgtgac 3061 aacacgccag ccctggagcc ccagccccag ggaaatgtcc catacctgga agcccaaaaa 3121 ggccagttcc tgcggctcct ctgtgctgct gacagccagc cccctgccac actgagctgg 3181 gtcctgcaga acagagtcct ctcctcgtcc catccctggg gccctagacc cctggggctg 3241 gagctgcccg gggtgaaggc tggggattca gggcgctaca cctgccgagc ggagaacagg 3301 cttggctccc agcagcgagc cctggacctc tctgtgcagt atcctccaga gaacctgaga 3361 gtgatggttt cccaagcaaa caggacagtc ctggaaaacc ttgggaacgg cacgtctctc 3421 ccagtactgg agggccaaag cctgtgcctg gtctgtgtca cacacagcag ccccccagcc 3481 aggctgagct ggacccagag gggacaggtt ctgagcccct cccagccctc agaccccggg 3541 gtcctggagc tgcctcgggt tcaagtggag cacgaaggag agttcacctg ccacgctcgg 3601 cacccactgg gctcccagca cgtctctctc agcctctccg tgcactactc cccgaagctg 3661 ctgggcccct cctgctcctg ggaggctgag ggtctgcact gcagctgctc ctcccaggcc 3721 agcccggccc cctctctgcg ctggtggctt ggggaggagc tgctggaggg gaacagcagc 3781 caggactcct tcgaggtcac ccccagctca gccgggccct gggccaacag ctccctgagc 3841 ctccatggag ggctcagctc cggcctcagg ctccgctgtg aggcctggaa cgtccatggg 3901 gcccagagtg gatccatcct gcagctgcca gataagaagg gactcatctc aacggcattc 3961 tccaacggag cgtttctggg aatcggcatc acggctcttc ttttcctctg cctggccctg 4021 atcatcatga agattctacc gaagagacgg actcagacag aaaccccgag gcccaggttc 4081 tcccggcaca gcacgatcct ggattacatc aatgtggtcc cgacggctgg ccccctggct 4141 cagaagcgga atcagaaagc cacaccaaac agtcctcgga cccctcttcc accaggtgct 4201 ccctccccag aatcaaagaa gaaccagaaa aagcagtatc agttgcccag tttcccagaa 4261 cccaaatcat ccactcaagc cccagaatcc caggagagcc aagaggagct ccattatgcc 4321 acgctcaact tcccaggcgt cagacccagg cctgaggccc ggatgcccaa gggcacccag 4381 gcggattatg cagaagtcaa gttccaagga tccgactaca aagaccatga cggtgattat 4441 aaagatcatg acatcgacta caaggatgac gatgacaagt agcggccgcg aaggatctgc 4501 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg 4561 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa 4621 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt 4681 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag 4741 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca 4801 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc 4861 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc 4921 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc 4981 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta 5041 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc 5101 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag 5161 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac 5221 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac 5281 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag 5341 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg 5401 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac 5461 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg 5521 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg 5581 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc 5641 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag 5701 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat 5761 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt 5821 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg 5881 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 5941 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 6001 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 6061 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 6121 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 6181 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 6241 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 6301 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 6361 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 6421 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa 6481 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 6541 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 6601 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 6661 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 6721 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 6781 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 6841 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt 6901 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 6961 cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt 7021 cggatctccc tttggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca 7081 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 7141 caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga 7201 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 7261 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 7321 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 7381 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca 7441 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 7501 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc 7561 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 7621 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 7681 gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt 7741 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 7801 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 7861 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 7921 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 7981 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 8041 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 8101 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 8161 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 8221 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 8281 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 8341 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 8401 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 8461 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 8521 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 8581 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 8641 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 8701 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 8761 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 8821 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 8881 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 8941 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 9001 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 9061 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 9121 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 9181 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 9241 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 9301 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 9361 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 9421 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 9481 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 9541 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 9601 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 9661 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 9721 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 9781 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 9841 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag 9901 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 9961 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 10021 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 10081 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 10141 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 10201 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 10261 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 10321 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg //