LOCUS       V025245                10364 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025245
VERSION     V025245
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4407
                     /label="SIGLEC10(NM_033130)"
                     /note="SIGLEC10(NM_033130)"
                     /gene="SIGLEC10"
     CDS             4414..4479
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4523..4734
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4747..5015
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5044..5053
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5074..5736
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5806..5859
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5860..6459
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6460..7047
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7121..7354
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7426..7547
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7566..7701
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7739..7755)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7763..7779
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7787..7817)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7832..7853
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8141..8729)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8900..9760)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9761..9865)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10339..10355
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc tactgccact gctgctgtcc
     2341 tcgctgctgg gcgggtccca ggctatggat gggagattct ggatacgagt gcaggagtca
     2401 gtgatggtgc cggagggcct gtgcatctct gtgccctgct ctttctccta cccccgacag
     2461 gactggacag ggtctacccc agcttatggc tactggttca aagcagtgac tgagacaacc
     2521 aagggtgctc ctgtggccac aaaccaccag agtcgagagg tggaaatgag cacccggggc
     2581 cgattccagc tcactgggga tcccgccaag gggaactgct ccttggtgat cagagacgcg
     2641 cagatgcagg atgagtcaca gtacttcttt cgggtggaga gaggaagcta tgtgagatat
     2701 aatttcatga acgatgggtt ctttctaaaa gtaacagccc tgactcagaa gcctgatgtc
     2761 tacatccccg agaccctgga gcccgggcag ccggtgacgg tcatctgtgt gtttaactgg
     2821 gcctttgagg aatgtccacc cccttctttc tcctggacgg gggctgccct ctcctcccaa
     2881 ggaaccaaac caacgacctc ccacttctca gtgctcagct tcacgcccag accccaggac
     2941 cacaacaccg acctcacctg ccatgtggac ttctccagaa agggtgtgag cgcacagagg
     3001 accgtccgac tccgtgtggc ctatgccccc agagaccttg ttatcagcat ttcacgtgac
     3061 aacacgccag ccctggagcc ccagccccag ggaaatgtcc catacctgga agcccaaaaa
     3121 ggccagttcc tgcggctcct ctgtgctgct gacagccagc cccctgccac actgagctgg
     3181 gtcctgcaga acagagtcct ctcctcgtcc catccctggg gccctagacc cctggggctg
     3241 gagctgcccg gggtgaaggc tggggattca gggcgctaca cctgccgagc ggagaacagg
     3301 cttggctccc agcagcgagc cctggacctc tctgtgcagt atcctccaga gaacctgaga
     3361 gtgatggttt cccaagcaaa caggacagtc ctggaaaacc ttgggaacgg cacgtctctc
     3421 ccagtactgg agggccaaag cctgtgcctg gtctgtgtca cacacagcag ccccccagcc
     3481 aggctgagct ggacccagag gggacaggtt ctgagcccct cccagccctc agaccccggg
     3541 gtcctggagc tgcctcgggt tcaagtggag cacgaaggag agttcacctg ccacgctcgg
     3601 cacccactgg gctcccagca cgtctctctc agcctctccg tgcactactc cccgaagctg
     3661 ctgggcccct cctgctcctg ggaggctgag ggtctgcact gcagctgctc ctcccaggcc
     3721 agcccggccc cctctctgcg ctggtggctt ggggaggagc tgctggaggg gaacagcagc
     3781 caggactcct tcgaggtcac ccccagctca gccgggccct gggccaacag ctccctgagc
     3841 ctccatggag ggctcagctc cggcctcagg ctccgctgtg aggcctggaa cgtccatggg
     3901 gcccagagtg gatccatcct gcagctgcca gataagaagg gactcatctc aacggcattc
     3961 tccaacggag cgtttctggg aatcggcatc acggctcttc ttttcctctg cctggccctg
     4021 atcatcatga agattctacc gaagagacgg actcagacag aaaccccgag gcccaggttc
     4081 tcccggcaca gcacgatcct ggattacatc aatgtggtcc cgacggctgg ccccctggct
     4141 cagaagcgga atcagaaagc cacaccaaac agtcctcgga cccctcttcc accaggtgct
     4201 ccctccccag aatcaaagaa gaaccagaaa aagcagtatc agttgcccag tttcccagaa
     4261 cccaaatcat ccactcaagc cccagaatcc caggagagcc aagaggagct ccattatgcc
     4321 acgctcaact tcccaggcgt cagacccagg cctgaggccc ggatgcccaa gggcacccag
     4381 gcggattatg cagaagtcaa gttccaagga tccgactaca aagaccatga cggtgattat
     4441 aaagatcatg acatcgacta caaggatgac gatgacaagt agcggccgcg aaggatctgc
     4501 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     4561 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     4621 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     4681 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     4741 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     4801 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     4861 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     4921 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     4981 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta
     5041 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc
     5101 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag
     5161 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac
     5221 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac
     5281 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag
     5341 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg
     5401 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac
     5461 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg
     5521 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg
     5581 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc
     5641 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag
     5701 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat
     5761 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt
     5821 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg
     5881 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     5941 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     6001 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     6061 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     6121 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     6181 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     6241 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     6301 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     6361 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     6421 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa
     6481 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6541 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6601 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6661 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6721 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6781 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     6841 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     6901 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6961 cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt
     7021 cggatctccc tttggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca
     7081 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     7141 caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     7201 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7261 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7321 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     7381 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     7441 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7501 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     7561 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7621 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     7681 gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt
     7741 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7801 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7861 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7921 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7981 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8041 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8101 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8161 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8221 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8281 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8341 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8401 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8461 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8521 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8581 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8641 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8701 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8761 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8821 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8881 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8941 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9001 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9061 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9121 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9181 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9241 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9301 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9361 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9421 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9481 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9541 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9601 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9661 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9721 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9781 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9841 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     9901 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     9961 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
    10021 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
    10081 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    10141 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
    10201 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    10261 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    10321 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
