LOCUS       V034046                10595 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034046
VERSION     V034046
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5427
                     /label="SIPA1(NM_006747)"
                     /note="SIPA1(NM_006747)"
                     /gene="SIPA1"
     CDS             5434..5460
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5464..5490
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5494..5520
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5565..5776
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5789..6057
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6084..6683
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6690..7278
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7352..7585
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7657..7778
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7797..7932
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7970..7986)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7994..8010
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8018..8048)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8063..8084
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8372..8960)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9131..9991)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9992..10096)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10570..10586
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgcccatg tgggccggcg gtgtggggag ccctcggcgg
     2341 ggcatggccc ctgcgtccac agatgacctc tttgcccgca agctgcgcca gccagcaagg
     2401 cccccgctga caccgcacac cttcgagccg aggccagtcc ggggcccact cctgcgcagc
     2461 ggcagcgatg caggcgaggc caggcccccc acgccagcca gcccccgtgc ccgtgcccac
     2521 agccacgaag aggccagccg acctgcagcc acttccaccc ggctcttcac tgacccgctg
     2581 gcactgctgg ggctgccagc agaggaacca gagcctgcct tcccaccagt gcttgagcct
     2641 cgatggtttg cccactatga cgtgcaaagc ctgctctttg attgggctcc gaggtctcag
     2701 gggatgggga gccactcaga ggccagctct gggaccctgg cttcagccga ggaccaggct
     2761 gccagctcgg acctgctgca tggggcacct ggctttgtgt gtgagctcgg gggtgagggt
     2821 gagctaggcc tgggtggacc agcatcccca cctgtgcccc ctgcactgcc caacgcggcc
     2881 gtgtccatcc tggaggagcc acagaaccga acctcggcct acagcctgga gcacgcagac
     2941 ctgggtgctg gctactaccg caaatacttc tatggcaaag aacatcagaa cttcttcggg
     3001 atggacgagt cgctgggccc ggtggcagtg agcctgcggc gggaggagaa ggagggcagc
     3061 ggagggggca ccctgcacag ctaccgcgtc atcgtgcgga ccacgcagct ccggacactc
     3121 cgtggcacca tctcggagga cgcgctgccg ccggggcccc cacggggtct gtccccaagg
     3181 aaacttctgg agcacgtggc gccgcagctg agccccagct gcctgcgcct gggctcagct
     3241 tcacccaagg taccacggac gctgctcaca ctggatgagc aagtgctgag cttccaacgc
     3301 aaggtgggca tcctgtactg ccgggcgggc cagggctcgg aggaggagat gtacaacaac
     3361 caggaggcgg gaccggcctt catgcagttt ctcaccttgc tgggcgatgt ggtgcggctc
     3421 aaaggctttg agagttaccg ggcccagcta gacaccaaaa cggattccac aggcacgcac
     3481 tccctctaca ccacatacca ggaccacgag atcatgttcc acgtgtccac gatgctgcct
     3541 tacaccccta ataaccagca gcagctcctc cggaagcgcc acattggcaa cgacattgtg
     3601 accatcgtgt tccaggagcc tggcagcaag cccttctgcc ccaccaccat ccgctcgcac
     3661 ttccagcacg tgttcctagt ggtgcgggca cacacaccct gcacgccaca caccacctac
     3721 agggtggccg tgagccgcac ccaggacacc cctgccttcg ggccagctct gcctgctggc
     3781 ggaggcccct tcgcagccaa cgccgacttc cgggccttcc tgctggccaa agcgctgaat
     3841 ggtgagcagg cggccggcca cgcgcgccag ttccacgcca tggccacgcg cacccgccag
     3901 cagtacctgc aagacctggc caccaacgag gtgaccacta cgtcgctgga ctcggcttca
     3961 cgcttcggcc tgccctccct gggtgggagg cgccgggcgg cccctcgggg cccaggcgcc
     4021 gagctgcagg cagcgggctc actggtgtgg ggagtgcgcg cggcgcccgg ggcgcgggtc
     4081 gccgccgggg ctcaggcgag cggccccgaa ggcatcgagg tgccctgcct gctgggcatc
     4141 tcggccgagg ctctggtgct ggtggcgccg cgcgacggcc gcgtagtgtt caattgcgcc
     4201 tgtcgcgacg tgctggcctg gaccttctcc gagcagcagc tggacctgta ccacggccgc
     4261 ggggaggcga tcacgctgcg cttcgacggg tcccccggcc aagccgtggg cgaggtggtg
     4321 gcgcgcctgc agctggtgag ccgtggctgc gagacccgcg agctggcgct gccccgcgac
     4381 ggtcaaggcc gcctgggctt cgaggtggac gccgagggat tcgtcacgca cgtggagcgc
     4441 ttcacattcg ccgagacggc ggggctgcgg cccggggcgc gcctcctgcg cgtgtgcggc
     4501 cagactctgc ccagcctccg gcccgaggcc gctgcccagc tcctgcgctc ggcgcccaag
     4561 gtctgcgtca ccgtcctgcc ccccgacgag agcggccggc cccgcaggag tttttcggag
     4621 ctgtacacgc tgtcgctgca ggagcctagc cggcgggggg ccccagatcc tgtgcaggat
     4681 gaggtccagg gggtgaccct gctgcccacc acaaagcagc tgctgcacct gtgcctgcaa
     4741 gatggtggca gtcctccagg gcctggggat ctggccgagg agaggactga gttcctgcac
     4801 agccagaact cgctgtcacc acgcagctct ctgtcggatg aggccccagt cctgcccaac
     4861 accaccccgg acctcctcct ggccaccaca gccaagccat cagtacccag tgctgacagt
     4921 gagacacccc tgacccagga caggccaggc agtcccagtg gctctgagga caagggcaac
     4981 ccggcgccgg agctgagggc ctcctttctg ccacgtacct tgtctctgcg gaactccatc
     5041 agcaggatca tgtcggaggc gggcagtggg accctggagg acgagtggca ggccatctcg
     5101 gagattgcct ctacttgcaa caccattctg gagtcgctgt cccgagaggg acagcccatc
     5161 ccagagagtg gagaccctaa gggaactcca aaatctgatg ctgagccaga gcctgggaac
     5221 ctctcagaga aggtctctca cttggagtcc atgctcagga agctgcagga ggacctgcag
     5281 aaggagaagg cggacagggc ggccctggag gaggaggtgc ggagcctgag acacaacaac
     5341 cggcggctgc aggcggagtc tgagagtgca gccacacgcc tcctcctggc ctccaagcag
     5401 ctgggctcac ccaccgccga cctggccgga tcctacccat acgacgtacc tgattatgca
     5461 acttatcctt atgatgtacc tgattatgct agctatcctt atgatgtccc agactacgca
     5521 taggcggccg cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc
     5581 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag
     5641 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     5701 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     5761 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg
     5821 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg
     5881 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct
     5941 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac
     6001 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa
     6061 gctgtgaccg gcgcctacgc tagatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     6121 gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     6181 cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     6241 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     6301 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     6361 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     6421 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     6481 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     6541 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     6601 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     6661 tgacccgcaa gcccggtgcc tgagtcgaca atcaacctct ggattacaaa atttgtgaaa
     6721 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     6781 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     6841 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     6901 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     6961 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7021 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7081 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7141 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7201 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7261 tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat
     7321 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa
     7381 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     7441 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7501 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7561 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     7621 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     7681 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7741 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     7801 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7861 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     7921 tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt
     7981 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8041 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8101 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8161 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8221 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8281 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8341 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8401 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8461 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8521 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8581 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8641 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8701 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8761 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     8821 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8881 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8941 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9001 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9061 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9121 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9181 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9241 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9301 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9361 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9421 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9481 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9541 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9601 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9661 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9721 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9781 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9841 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9901 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9961 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10021 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10081 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
    10141 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
    10201 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
    10261 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    10321 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
    10381 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
    10441 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    10501 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    10561 cacgacgttg taaaacgacg gccagtgcca agctg
//
