LOCUS       V034271                10228 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034271
VERSION     V034271
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4543
                     /codon_start=1
                     /gene=""
                     /note="homolog of budding yeast Sir2"
                     /product="human NAD+-dependent protein deacetylase
                     sirtuin-1"
                     /protein_id=""
                     /transl_table=1
                     /translation="MADEAALALQPGGSPSAAGADREAASSPAGEPLRKRPRRDGPGLE
                     RSPGEPGGAAPEREVPAAARGCPGAAAAALWREAEAEAAAAGGEQEAQATAAAGEGDNG
                     PGLQGPSREPPLADNLYDEDDDDEGEEEEEAAAAAIGYRDNLLFGDEIITNGFHSCESD
                     EEDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQ
                     IVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYA
                     RLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLL
                     RNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCP
                     ADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHE
                     VPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRT
                     QKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCME
                     EKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISR
                     RLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESE
                     IEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS"
                     /label="SIRT1"
     CDS             4550..4576
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4621..4832
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4845..5113
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5148..5855
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5865..5918
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5919..6317
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     misc_feature    6324..6911
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6985..7218
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7290..7411
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7430..7565
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7603..7619)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7627..7643
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7651..7681)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7696..7717
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8005..8593)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8764..9624)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9625..9729)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10203..10219
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggcgga cgaggcggcc ctcgcccttc agcccggcgg
     2341 ctccccctcg gcggcggggg ccgacaggga ggccgcgtcg tcccccgccg gggagccgct
     2401 ccgcaagagg ccgcggagag atggtcccgg cctcgagcgg agcccgggcg agcccggtgg
     2461 ggcggcccca gagcgtgagg tgccggcggc ggccaggggc tgcccgggtg cggcggcggc
     2521 ggcgctgtgg cgggaggcgg aggcagaggc ggcggcggca ggcggggagc aagaggccca
     2581 ggcgactgcg gcggctgggg aaggagacaa tgggccgggc ctgcagggcc catctcggga
     2641 gccaccgctg gccgacaact tgtacgacga agacgacgac gacgagggcg aggaggagga
     2701 agaggcggcg gcggcggcga ttgggtaccg agataacctt ctgttcggtg atgaaattat
     2761 cactaatggt tttcattcct gtgaaagtga tgaggaggat agagcctcac atgcaagctc
     2821 tagtgactgg actccaaggc cacggatagg tccatatact tttgttcagc aacatcttat
     2881 gattggcaca gatcctcgaa caattcttaa agatttattg ccggaaacaa tacctccacc
     2941 tgagttggat gatatgacac tgtggcagat tgttattaat atcctttcag aaccaccaaa
     3001 aaggaaaaaa agaaaagata ttaatacaat tgaagatgct gtgaaattac tgcaagagtg
     3061 caaaaaaatt atagttctaa ctggagctgg ggtgtctgtt tcatgtggaa tacctgactt
     3121 caggtcaagg gatggtattt atgctcgcct tgctgtagac ttcccagatc ttccagatcc
     3181 tcaagcgatg tttgatattg aatatttcag aaaagatcca agaccattct tcaagtttgc
     3241 aaaggaaata tatcctggac aattccagcc atctctctgt cacaaattca tagccttgtc
     3301 agataaggaa ggaaaactac ttcgcaacta tacccagaac atagacacgc tggaacaggt
     3361 tgcgggaatc caaaggataa ttcagtgtca tggttccttt gcaacagcat cttgcctgat
     3421 ttgtaaatac aaagttgact gtgaagctgt acgaggagat atttttaatc aggtagttcc
     3481 tcgatgtcct aggtgcccag ctgatgaacc gcttgctatc atgaaaccag agattgtgtt
     3541 ttttggtgaa aatttaccag aacagtttca tagagccatg aagtatgaca aagatgaagt
     3601 tgacctcctc attgttattg ggtcttccct caaagtaaga ccagtagcac taattccaag
     3661 ttccataccc catgaagtgc ctcagatatt aattaataga gaacctttgc ctcatctgca
     3721 ttttgatgta gagcttcttg gagactgtga tgtcataatt aatgaattgt gtcataggtt
     3781 aggtggtgaa tatgccaaac tttgctgtaa ccctgtaaag ctttcagaaa ttactgaaaa
     3841 acctccacga acacaaaaag aattggctta tttgtcagag ttgccaccca cacctcttca
     3901 tgtttcagaa gactcaagtt caccagaaag aacttcacca ccagattctt cagtgattgt
     3961 cacactttta gaccaagcag ctaagagtaa tgatgattta gatgtgtctg aatcaaaagg
     4021 ttgtatggaa gaaaaaccac aggaagtaca aacttctagg aatgttgaaa gtattgctga
     4081 acagatggaa aatccggatt tgaagaatgt tggttctagt actggggaga aaaatgaaag
     4141 aacttcagtg gctggaacag tgagaaaatg ctggcctaat agagtggcaa aggagcagat
     4201 tagtaggcgg cttgatggta atcagtatct gtttttgcca ccaaatcgtt acattttcca
     4261 tggcgctgag gtatattcag actctgaaga tgacgtctta tcctctagtt cttgtggcag
     4321 taacagtgat agtgggacat gccagagtcc aagtttagaa gaacccatgg aggatgaaag
     4381 tgaaattgaa gaattctaca atggcttaga agatgagcct gatgttccag agagagctgg
     4441 aggagctgga tttgggactg atggagatga tcaagaggca attaatgaag ctatatctgt
     4501 gaaacaggaa gtaacagaca tgaactatcc atcaaacaaa tcaggatcct acccatacga
     4561 cgtaccagat tacgcttgag cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt
     4621 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     4681 cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     4741 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     4801 tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc
     4861 cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc
     4921 cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca
     4981 ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct
     5041 ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc
     5101 gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg gtgagcaagg
     5161 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     5221 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     5281 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     5341 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     5401 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     5461 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     5521 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     5581 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     5641 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     5701 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     5761 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     5821 gccactccac cggcggcatg gacgagctgt acaagggatc tcgcgagggc agaggaagtc
     5881 ttctaacatg cggtgacgtg gaggagaatc ccggccctat ggccaagcct ttgtctcaag
     5941 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
     6001 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
     6061 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
     6121 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
     6181 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
     6241 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
     6301 atgtgtggga gggctaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     6361 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6421 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6481 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     6541 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     6601 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     6661 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     6721 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     6781 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     6841 ctctgcggcc tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggc
     6901 cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6961 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc
     7021 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7081 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7141 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7201 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     7261 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     7321 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7381 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     7441 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7501 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7561 aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt
     7621 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7681 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7741 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7801 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7861 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7921 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7981 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8041 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8101 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8161 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8221 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8281 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8341 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8401 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     8461 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8521 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8581 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8641 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8701 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8761 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8821 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8881 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8941 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9001 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9061 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9121 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9181 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9241 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9301 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9361 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9421 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9481 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9541 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9601 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9661 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9721 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     9781 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     9841 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     9901 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     9961 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
    10021 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
    10081 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    10141 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    10201 ttgtaaaacg acggccagtg ccaagctg
//
