LOCUS       V042333                11185 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042333
VERSION     V042333
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..6039
                     /label="SKIC2(NM_006929)"
                     /note="SKIC2(NM_006929)"
                     /gene="SKIC2"
     CDS             6046..6111
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6155..6366
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6379..6647
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6674..7273
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7280..7868
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7942..8175
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8247..8368
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8387..8522
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8560..8576)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8584..8600
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8608..8638)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8653..8674
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8962..9550)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9721..10581)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10582..10686)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11160..11176
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgatggag acagagcgac ttgtgctacc ccctccagat
     2341 cccctggacc taccccttcg ggccgtggag ctcggatgca cggggcactg ggagctgctg
     2401 aacttgcctg gagctccaga gagtagcctt ccccatggcc tccctccttg tgccccagat
     2461 ctgcagcaag aagcagaaca gttgtttctg tcatccccag cctggctgcc tctgcatggt
     2521 gtggagcact cagcccgaaa atggcagagg aagacggatc cctggtctct tttggctgtc
     2581 ctgggagccc cagtcccatc cgacctacag gcccaaagac acccaaccac aggccagata
     2641 ctgggttaca aagaggtctt gctggagaac acaaatctct cggctacaac ctccttgtct
     2701 cttcgccggc ctccagggcc agcctcccag tccttatggg gaaatccaac tcagtatccc
     2761 ttctggccag gggggatgga tgaacccacc ataacagatc tgaacacacg ggaggaggct
     2821 gaggaggaga tagactttga gaaagatctt cttactattc cacctggttt caagaaaggc
     2881 atggactttg caccaaaaga ttgtccaact ccagctcctg gactactaag ccttagctgt
     2941 atgttggagc ctctggattt gggtgggggt gacgaggatg agaatgaggc agtgggacag
     3001 ccaggaggtc ccagagggga cactgtttca gcctctccct gcagtgctcc cctggcccga
     3061 gcaagcagct tggaagacct agtgttgaag gaagcgtcca cagctgtatc caccccagag
     3121 gccccagagc ctccatctca ggagcagtgg gccatccctg tggacgccac ctcccctgtt
     3181 ggtgatttct atcgcctcat tccccagcca gccttccagt gggcatttga gccagatgtg
     3241 tttcagaaac aggccatcct gcacttggaa cggcatgact ctgtctttgt cgcagctcac
     3301 acatctgcag gaaaaacagt tgtggctgaa tatgccattg ccctggccca gaaacacatg
     3361 acacgcacca tctacacttc gcccatcaag gccctgagca accagaagtt ccgggacttc
     3421 cgaaacacat tcggggatgt ggggctgctc accggggatg tacagctgca tccggaggcc
     3481 tcctgcctca tcatgaccac agagatcctt cgctccatgc tgtacagtgg ctcagatgtt
     3541 attcgggacc tggagtgggt catctttgat gaggttcact atatcaacga tgtcgagcgt
     3601 ggggtcgtgt gggaggaggt gcttatcatg ctacctgacc acgtttctat catccttctg
     3661 agtgccaccg tccccaacgc ccttgagttt gctgactgga ttgggcggct gaagcgtcgt
     3721 cagatctatg tgattagcac tgtaacccgc cccgtgcccc tggagcacta tcttttcaca
     3781 gggaacagct ccaagaccca gggggagctc tttttgttgc tggactcccg aggagccttc
     3841 catacaaaag ggtactatgc agctgtggag gccaagaagg agagaatgag caaacacgcc
     3901 cagacctttg gggccaagca gcccacacat caggggggcc ctgcacagga ccgcggagtg
     3961 tacctgtccc tcctggcctc cctccgcaca cgtgcccagt tgcccgtggt ggtgttcacc
     4021 ttctcccggg gccgctgtga tgagcaggcc tcaggcctca cctcccttga cctcaccacc
     4081 agttcggaga agagcgagat ccacctcttc ctgcagcgct gccttgctcg cctccgtggc
     4141 tctgaccgcc agctgcccca ggtcctgcac atgtcagagc tcctgaatcg cggcctgggt
     4201 gtgcaccata gcggcatcct gcccatcctc aaggagatcg tggagatgct cttcagccgt
     4261 ggcctggtca aggtcttgtt tgccacagag acctttgcca tgggagtaaa catgcctgct
     4321 cgtacagtag tgtttgactc catgcgcaaa cacgatggct ccaccttccg ggacctgctc
     4381 cctggggagt atgtgcagat ggcaggccgg gcagggcgga ggggcctgga ccccacaggc
     4441 accgttatcc tgctctgcaa gggccgagtg cccgagatgg cagacctgca ccgcatgatg
     4501 atggggaagc cgtcccagct gcagtcccag ttccgcctca cgtacactat gatcctcaac
     4561 ttgctgcgag tggatgccct cagggtggag gacatgatga agaggagctt ctctgagttt
     4621 ccctcccgca aagacagcaa ggcccatgaa caggccctgg ctgaactgac caagaggctg
     4681 ggagctttgg aggagcctga catgactggc caactggtcg acctgcctga atattacagc
     4741 tggggggagg aactgacaga gacccagcac atgatccagc gacgcatcat ggagtctgtg
     4801 aacgggctga agtctctctc agcaggaagg gtggtggttg tgaagaatca ggagcatcac
     4861 aacgcattgg gagtgatcct acaggtctcc tcgaactcca ccagcagagt attcacaacc
     4921 ctggtcttgt gtgataagcc cttgtcccag gacccacagg acagggggcc agccactgca
     4981 gaggtgccct atccagatga cctcgtggga ttcaagctgt tcctgcctga agggccttgt
     5041 gaccacaccg tggtcaagct ccagccagga gatatggctg ccatcaccac caaggtgctc
     5101 cgggtgaatg gggagaagat cttggaggac ttcagcaaga ggcagcagcc aaaattcaag
     5161 aaggatcctc cccttgcagc cgtgaccact gctgtccagg aactgctgcg tctggctcag
     5221 gcccacccag ccggacctcc caccctcgac cctgtcaatg acctgcagct caaagatatg
     5281 tcagttgtag agggtgggct ccgggcccgg aagctggagg agctgatcca gggggctcag
     5341 tgtgtacaca gcccccgttt tcctgcccag tacctgaagc tgcgggagcg aatgcagata
     5401 cagaaggaga tggagcggct gcgcttccta ctgtcggatc agtcattgct gctgcttcct
     5461 gagtaccatc agcgagtaga ggtgctccga accctgggtt atgtggacga ggcgggcact
     5521 gtgaagctgg cagggcgggt ggcttgtgcc atgagcagcc atgagttgct cctcactgag
     5581 ctcatgtttg acaatgcact gagcaccctg cggcctgagg agattgctgc cttgctctct
     5641 ggcctggtct gccagagccc tggggacgct ggggatcagc tcccaaacac cctcaagcag
     5701 ggaatagaac gtgtccgggc tgtggccaag cggattggtg aggtccaggt ggcttgtggc
     5761 ctgaaccaga cggtggagga atttgtgggg gagctgaatt ttgggctggt tgaggttgta
     5821 tatgagtggg cccggggcat gcccttctcc gagttggcag ggctctcagg gacccctgag
     5881 ggcctggtgg tccgctgcat tcagcgcctg gctgagatgt gtcgctcact gcggggggca
     5941 gcccgcctgg taggagagcc tgtgctgggt gccaagatgg agacagcggc taccttgcta
     6001 cggcgggaca tcgtatttgc ggccagcctc tacacccagg gatccgacta caaagaccat
     6061 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagcggccg
     6121 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     6181 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     6241 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     6301 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     6361 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     6421 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     6481 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     6541 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     6601 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     6661 gcgcctacgc tagatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     6721 cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     6781 cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     6841 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     6901 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     6961 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     7021 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     7081 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     7141 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     7201 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     7261 gcccggtgcc tgagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7321 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7381 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7441 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7501 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7561 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7621 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     7681 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7741 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7801 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7861 ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7921 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc
     7981 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8041 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8101 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8161 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     8221 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     8281 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     8341 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     8401 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8461 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     8521 cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg
     8581 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8641 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8701 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8761 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8821 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8881 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8941 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9001 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9061 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9121 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9181 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9241 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9301 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9361 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     9421 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9481 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9541 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9601 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9661 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9721 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9781 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9841 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9901 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9961 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    10021 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10081 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10141 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10201 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10261 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10321 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10381 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10441 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10501 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10561 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10621 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10681 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
    10741 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
    10801 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    10861 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
    10921 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
    10981 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
    11041 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
    11101 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    11161 taaaacgacg gccagtgcca agctg
//
