LOCUS V029963 8402 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029963 VERSION V029963 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..3234 /label="SLC25A1(NM_005984)" /note="SLC25A1(NM_005984)" /gene="SLC25A1" CDS 3241..3267 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3271..3297 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3301..3327 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 3372..3583 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3596..3864 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 3891..4490 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 4497..5085 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5159..5392 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 5464..5585 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 5604..5739 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(5777..5793) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5801..5817 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5825..5855) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5870..5891 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6179..6767) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6938..7798) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7799..7903) /gene="bla" /label="AmpR promoter" primer_bind 8377..8393 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catgcccgcg ccccgcgccc cgcgcgctct ggcggccgcc 2341 gcgcccgcgt ccgggaaggc caagctgacg cacccgggga aggcgatcct ggcaggcggc 2401 ctggcgggtg gcatcgagat ctgcatcacc ttccccaccg agtacgtgaa gacgcagctg 2461 cagctggacg agcgctcgca cccgccgcgg taccggggca tcggggactg cgtgcggcag 2521 acggttcgca gccatggcgt cctgggcctg taccgcggcc ttagctccct gctctacggt 2581 tccatcccca aggcggccgt caggtttgga atgttcgagt tcctcagcaa ccacatgcgg 2641 gatgcccagg gacggctgga cagcacgcgt gggctgctgt gcggcctggg cgctggcgtg 2701 gccgaggccg tggtggtcgt gtgccccatg gagaccatca aggtgaagtt catccacgac 2761 cagacctccc caaaccccaa gtacagagga ttcttccacg gggttaggga gattgtgcgg 2821 gaacaagggc tgaaggggac gtaccagggc ctcacagcca ctgtcctgaa gcagggctcg 2881 aaccaggcca tccgcttctt cgtcatgacc tccctgcgca actggtaccg aggggacaac 2941 cccaacaagc ccatgaaccc tctgatcact ggggtcttcg gagctattgc aggcgcagcc 3001 agtgtctttg gaaacactcc tctggatgtg attaagaccc ggatgcaggg cctggaggcg 3061 cacaaatacc ggaacacgtg ggactgcggc ttgcagatcc tgaagaagga ggggctcaag 3121 gcattctaca agggcactgt cccccgcctg ggccgggtct gcctggatgt ggccatagtg 3181 tttgtcatct atgatgaagt ggtgaagctg ctcaacaaag tgtggaagac ggacggatcc 3241 tacccatacg acgtacctga ttatgcaact tatccttatg atgtacctga ttatgctagc 3301 tatccttatg atgtcccaga ctacgcatag gcggccgcga aggatctgcg atcgctccgg 3361 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 3421 cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 3481 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 3541 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc 3601 tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag 3661 tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag 3721 tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact 3781 cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt 3841 ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag atgaccgagt 3901 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg 3961 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg 4021 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg 4081 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag 4141 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg 4201 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt 4261 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg 4321 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg 4381 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg 4441 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gtcgacaatc 4501 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 4561 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 4621 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 4681 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 4741 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 4801 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 4861 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct 4921 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 4981 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 5041 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga 5101 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 5161 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc 5221 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 5281 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 5341 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 5401 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 5461 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 5521 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 5581 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc 5641 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 5701 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc 5761 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 5821 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 5881 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 5941 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 6001 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 6061 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 6121 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 6181 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 6241 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 6301 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 6361 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 6421 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 6481 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 6541 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 6601 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 6661 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 6721 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 6781 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 6841 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 6901 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 6961 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 7021 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 7081 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 7141 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 7201 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 7261 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 7321 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 7381 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 7441 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 7501 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 7561 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 7621 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 7681 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 7741 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 7801 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 7861 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 7921 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 7981 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 8041 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 8101 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 8161 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 8221 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 8281 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 8341 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc 8401 tg //