LOCUS       V026014                10345 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026014
VERSION     V026014
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4371
                     /label="SLC3A1(NM_000341)"
                     /note="SLC3A1(NM_000341)"
                     /gene="SLC3A1"
     CDS             4378..4443
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4487..4698
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4711..4979
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5008..5017
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5038..5700
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5770..5823
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5824..6423
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6424..7012
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7086..7319
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7391..7512
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7552..7687
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7720..7736)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7744..7760
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7768..7798)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7813..7834
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8122..8710)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8881..9741)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9742..9846)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10320..10336
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ctgaagataa aagcaagaga
     2341 gactccatcg agatgagtat gaagggatgc cagacaaaca acgggtttgt ccataatgaa
     2401 gacattctgg agcagacccc ggatccagga agctcaacag acaacctgaa gcacagcacc
     2461 aggggcatcc ttggctccca ggagcccgac ttcaagggcg tccagcccta tgcggggatg
     2521 cccaaggagg tgctgttcca gttctctggc caggcccgct accgcatacc tcgggagatc
     2581 ctcttctggc tcacagtggc ttctgtgctg gtgctcatcg cggccaccat agccatcatt
     2641 gccctctctc caaagtgcct agactggtgg caggaggggc ccatgtacca gatctaccca
     2701 aggtctttca aggacagtaa caaggatggg aacggagatc tgaaaggtat tcaagataaa
     2761 ctggactaca tcacagcttt aaatataaaa actgtttgga ttacttcatt ttataaatcg
     2821 tcccttaaag atttcagata tggtgttgaa gatttccggg aagttgatcc catttttgga
     2881 acgatggaag attttgagaa tctggttgca gccatacatg ataaaggttt aaaattaatc
     2941 atcgatttca taccaaacca cacgagtgat aaacatattt ggtttcaatt gagtcggaca
     3001 cggacaggaa aatatactga ttattatatc tggcatgact gtacccatga aaatggcaaa
     3061 accattccac ccaacaactg gttaagtgtg tatggaaact ccagttggca ctttgacgaa
     3121 gtgcgaaacc aatgttattt tcatcagttt atgaaagagc aacctgattt aaatttccgc
     3181 aatcctgatg ttcaagaaga aataaaagaa attttacggt tctggctcac aaagggtgtt
     3241 gatggtttta gtttggatgc tgttaaattc ctcctagaag caaagcacct gagagatgag
     3301 atccaagtaa ataagaccca aatcccggac acggtcacac aatactcgga gctgtaccat
     3361 gacttcacca ccacgcaggt gggaatgcac gacattgtcc gcagcttccg gcagaccatg
     3421 gaccaataca gcacggagcc cggcagatac aggttcatgg ggactgaagc ctatgcagag
     3481 agtattgaca ggaccgtgat gtactatgga ttgccattta tccaagaagc tgattttccc
     3541 ttcaacaatt acctcagcat gctagacact gtttctggga acagcgtgta tgaggttatc
     3601 acatcctgga tggaaaacat gccagaagga aaatggccta actggatgat tggtggacca
     3661 gacagttcac ggctgacttc gcgtttgggg aatcagtatg tcaacgtgat gaacatgctt
     3721 cttttcacac tccctggaac tcctataact tactatggag aagaaattgg aatgggaaat
     3781 attgtagccg caaatctcaa tgaaagctat gatattaata cccttcgctc aaagtcacca
     3841 atgcagtggg acaatagttc aaatgctggt ttttctgaag ctagtaacac ctggttacct
     3901 accaattcag attaccacac tgtgaatgtt gatgtccaaa agactcagcc cagatcggct
     3961 ttgaagttat atcaagattt aagtctactt catgccaatg agctactcct caacaggggc
     4021 tggttttgcc atttgaggaa tgacagccac tatgttgtgt acacaagaga gctggatggc
     4081 atcgacagaa tctttatcgt ggttctgaat tttggagaat caacactgtt aaatctacat
     4141 aatatgattt cgggccttcc cgctaaaatg agaataaggt taagtaccaa ttctgccgac
     4201 aaaggcagta aagttgatac aagtggcatt tttctggaca agggagaggg actcatcttt
     4261 gaacacaaca cgaagaatct ccttcatcgc caaacagctt tcagagatag atgctttgtt
     4321 tccaatcgag catgctattc cagtgtactg aacatactgt atacctcgtg tggatccgac
     4381 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
     4441 aagtagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca
     4501 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga
     4561 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     4621 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     4681 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc
     4741 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
     4801 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc
     4861 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
     4921 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc
     4981 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc
     5041 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg
     5101 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa
     5161 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac
     5221 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc
     5281 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc
     5341 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc
     5401 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg
     5461 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac
     5521 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc
     5581 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc
     5641 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc
     5701 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc
     5761 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
     5821 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     5881 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     5941 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     6001 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     6061 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     6121 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     6181 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     6241 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     6301 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     6361 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     6421 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     6481 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     6541 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6601 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     6661 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6721 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6781 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     6841 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6901 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6961 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc
     7021 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     7081 gggactggaa gggctaattc actcccaacg aagataagat ctgctttttg cttgtactgg
     7141 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7201 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7261 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7321 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     7381 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7441 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7501 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     7561 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7621 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7681 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg
     7741 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7801 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7861 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7921 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7981 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8041 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8101 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8161 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8221 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8281 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8341 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8401 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8461 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8521 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     8581 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8641 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8701 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8761 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8821 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8881 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8941 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9001 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9061 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9121 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9181 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9241 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9301 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9361 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9421 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9481 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9541 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9601 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9661 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9721 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9781 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9841 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     9901 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     9961 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    10021 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
    10081 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
    10141 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
    10201 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
    10261 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    10321 taaaacgacg gccagtgcca agctg
//
