LOCUS V043574 8183 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V043574 VERSION V043574 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..3015 /label="SMNDC1(NM_005871)" /note="SMNDC1(NM_005871)" /gene="SMNDC1" CDS 3022..3048 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3052..3078 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3082..3108 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 3153..3364 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3377..3645 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 3672..4271 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 4278..4866 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 4940..5173 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 5245..5366 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 5385..5520 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(5558..5574) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5582..5598 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5606..5636) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5651..5672 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5960..6548) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6719..7579) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7580..7684) /gene="bla" /label="AmpR promoter" primer_bind 8158..8174 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catgtcagag gatttagcaa agcagctggc aagctacaaa 2341 gctcagctcc agcaagttga agctgcatta tctggaaatg gagaaaatga agatttgcta 2401 aaattgaaga aagatttaca agaagttata gaactaacca aagaccttct gtcaactcaa 2461 ccttctgaga cgcttgcaag ttcagacagt tttgcttcta ctcaacctac tcattcatgg 2521 aaagtaggag acaagtgtat ggcagtctgg agtgaagatg gacagtgtta tgaagcggag 2581 attgaggaga tagatgaaga aaatggcacc gctgcaatca cctttgctgg ttatggcaat 2641 gctgaagtga ctccactgtt gaacctcaag cctgtagaag aaggaaggaa ggcaaaggag 2701 gacagtggca acaaacccat gtcaaaaaaa gaaatgattg cccagcagcg tgaatataaa 2761 aagaagaaag ctttgaaaaa agctcagaga ataaaagaac ttgagcagga aagagaggac 2821 cagaaagtga aatggcaaca attcaacaac agagcctatt ctaaaaacaa aaaaggccag 2881 gtaaagagga gtatttttgc ttcacctgag agtgtgactg gtaaagttgg agtaggaacc 2941 tgtggaattg ctgataaacc tatgacacaa tatcaagata cctctaaata caatgtcagg 3001 catttgatgc ctcaaggatc ctacccatac gacgtacctg attatgcaac ttatccttat 3061 gatgtacctg attatgctag ctatccttat gatgtcccag actacgcata ggcggccgcg 3121 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc 3181 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt 3241 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc 3301 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac 3361 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg 3421 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac 3481 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct 3541 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca 3601 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc 3661 gcctacgcta gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc 3721 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg 3781 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg 3841 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga 3901 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg 3961 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc 4021 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca 4081 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc 4141 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca 4201 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc 4261 ccggtgcctg agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta 4321 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 4381 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 4441 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 4501 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 4561 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 4621 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt 4681 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 4741 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 4801 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct 4861 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 4921 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt 4981 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 5041 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 5101 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 5161 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 5221 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 5281 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 5341 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 5401 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 5461 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 5521 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa 5581 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 5641 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 5701 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 5761 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 5821 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 5881 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 5941 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 6001 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 6061 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 6121 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 6181 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 6241 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 6301 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 6361 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 6421 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 6481 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 6541 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 6601 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 6661 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 6721 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 6781 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 6841 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 6901 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 6961 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 7021 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 7081 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 7141 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 7201 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 7261 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 7321 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 7381 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 7441 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 7501 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 7561 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 7621 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 7681 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 7741 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg 7801 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 7861 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc 7921 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 7981 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca 8041 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 8101 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta 8161 aaacgacggc cagtgccaag ctg //