LOCUS V032983 8705 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V032983 VERSION V032983 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" regulatory 2302..2311 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 2311..2352 /codon_start=1 /product="epitope tag from simian virus 5" /transl_table=1 /translation="GKPIPNPLLGLDST" /label="V5 tag" CDS 2353..3624 /label="STAMBP(NM_006463)" /note="STAMBP(NM_006463)" /gene="STAMBP" promoter 3675..3886 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3899..4167 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4194..4793 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 4800..5388 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5462..5695 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 5767..5888 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 5907..6042 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6080..6096) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6104..6120 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6128..6158) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6173..6194 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6482..7070) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7241..8101) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8102..8206) /gene="bla" /label="AmpR promoter" primer_bind 8680..8696 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac cgccaccatg ggtaagccta tccctaaccc tctcctcggt 2341 ctcgattcta caatgtctga ccatggagat gtgagcctcc cgcccgaaga ccgggtgagg 2401 gctctctccc agctgggtag tgcggtagag gtgaatgaag acattccacc ccgtcggtac 2461 ttccgctctg gagttgagat tatccgaatg gcatccattt actctgagga aggcaacatt 2521 gaacatgcct tcatcctcta taacaagtat atcacgctct ttattgagaa actaccaaaa 2581 catcgagatt acaaatctgc tgtcattcct gaaaagaaag acacagtaaa gaaattaaag 2641 gagattgcat ttcccaaagc agaagagctg aaggcagagc tgttaaaacg atataccaaa 2701 gaatatacag aatataatga agaaaagaag aaggaagcag aggaattggc ccggaacatg 2761 gccatccagc aagagctgga aaaggaaaaa cagagggtag cacaacagaa gcagcagcaa 2821 ttggaacagg aacagttcca tgccttcgag gagatgatcc ggaaccagga gctagaaaaa 2881 gagcgactga aaattgtaca ggagtttggg aaggtagacc ctggcctagg tggcccgcta 2941 gtgcctgact tggagaagcc ctccttagat gtgttcccca ccttaacagt ctcatccata 3001 cagccttcag actgtcacac aactgtaagg ccagctaagc cacctgtggt ggacaggtcc 3061 ttgaaacctg gagcactgag caactcagaa agtattccca caatcgatgg attgcgccat 3121 gtggtggtgc ctgggcggct gtgcccacag tttctccagt tagccagtgc caacactgcc 3181 cggggagtgg agacatgtgg aattctctgt ggaaaactga tgaggaatga atttaccatt 3241 acccatgttc tcatccccaa gcaaagtgct gggtctgatt actgcaacac agagaacgaa 3301 gaagaacttt tcctcataca ggatcagcag ggcctcatca cactgggctg gattcatact 3361 caccccacac agaccgcgtt tctctccagt gtcgacctac acactcactg ctcttaccag 3421 atgatgttgc cagagtcagt agccattgtt tgctccccca agttccagga aactggattc 3481 tttaaactaa ctgaccatgg actagaggag atttcttcct gtcgccagaa aggatttcat 3541 ccacacagca aggatccacc tctgttctgt agctgcagcc acgtgactgt tgtggacaga 3601 gcagtgacca tcacagacct tcgataggga tccgcggccg cgaaggatct gcgatcgctc 3661 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 3721 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 3781 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 3841 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg 3901 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt 3961 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt 4021 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag 4081 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc 4141 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagatgaccg 4201 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc 4261 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca 4321 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca 4381 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg 4441 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc 4501 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt 4561 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg 4621 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct 4681 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg 4741 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca 4801 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 4861 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 4921 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 4981 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 5041 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 5101 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 5161 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 5221 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 5281 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 5341 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta 5401 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 5461 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 5521 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 5581 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 5641 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 5701 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 5761 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 5821 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 5881 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 5941 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 6001 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 6061 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 6121 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 6181 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 6241 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 6301 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 6361 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 6421 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 6481 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 6541 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 6601 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 6661 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 6721 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 6781 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 6841 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 6901 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 6961 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 7021 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 7081 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 7141 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 7201 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 7261 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 7321 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 7381 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 7441 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 7501 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 7561 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 7621 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 7681 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 7741 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 7801 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 7861 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 7921 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 7981 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 8041 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 8101 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 8161 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 8221 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 8281 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 8341 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 8401 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 8461 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 8521 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 8581 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 8641 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 8701 agctg //