LOCUS       V036671                10651 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036671
VERSION     V036671
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4677
                     /label="STAT5B(NM_012448)"
                     /note="STAT5B(NM_012448)"
                     /gene="STAT5B"
     CDS             4684..4749
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4793..5004
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5017..5285
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5314..5323
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5344..6006
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6076..6129
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6130..6729
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6730..7318
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7392..7625
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7697..7818
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7858..7993
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8026..8042)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8050..8066
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8074..8104)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8119..8140
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8428..9016)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9187..10047)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10048..10152)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10626..10642
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ctgtgtggat acaagctcag
     2341 cagctccaag gagaagccct tcatcagatg caagcgttat atggccagca ttttcccatt
     2401 gaggtgcggc attatttatc ccagtggatt gaaagccaag catgggactc agtagatctt
     2461 gataatccac aggagaacat taaggccacc cagctcctgg agggcctggt gcaggagctg
     2521 cagaagaagg cagagcacca ggtgggggaa gatgggtttt tactgaagat caagctgggg
     2581 cactatgcca cacagctcca gaacacgtat gaccgctgcc ccatggagct ggtccgctgc
     2641 atccgccata tattgtacaa tgaacagagg ttggtccgag aagccaacaa tggtagctct
     2701 ccagctggaa gccttgctga tgccatgtcc cagaaacacc tccagatcaa ccagacgttt
     2761 gaggagctgc gactggtcac gcaggacaca gagaatgagt taaaaaagct gcagcagact
     2821 caggagtact tcatcatcca gtaccaggag agcctgagga tccaagctca gtttggcccg
     2881 ctggcccagc tgagccccca ggagcgtctg agccgggaga cggccctcca gcagaagcag
     2941 gtgtctctgg aggcctggtt gcagcgtgag gcacagacac tgcagcagta ccgcgtggag
     3001 ctggccgaga agcaccagaa gaccctgcag ctgctgcgga agcagcagac catcatcctg
     3061 gatgacgagc tgatccagtg gaagcggcgg cagcagctgg ccgggaacgg cgggcccccc
     3121 gagggcagcc tggacgtgct acagtcctgg tgtgagaagt tggccgagat catctggcag
     3181 aaccggcagc agatccgcag ggctgagcac ctctgccagc agctgcccat ccccggccca
     3241 gtggaggaga tgctggccga ggtcaacgcc accatcacgg acattatctc agccctggtg
     3301 accagcacgt tcatcattga gaagcagcct cctcaggtcc tgaagaccca gaccaagttt
     3361 gcagccactg tgcgcctgct ggtgggcggg aagctgaacg tgcacatgaa ccccccccag
     3421 gtgaaggcca ccatcatcag tgagcagcag gccaagtctc tgctcaagaa cgagaacacc
     3481 cgcaatgatt acagtggcga gatcttgaac aactgctgcg tcatggagta ccaccaagcc
     3541 acaggcaccc ttagtgccca cttcaggaat atgtccctga aacgaattaa gaggtcagac
     3601 cgtcgtgggg cagagtcggt gacagaagaa aaatttacaa tcctgtttga atcccagttc
     3661 agtgttggtg gaaatgagct ggtttttcaa gtcaagaccc tgtccctgcc agtggtggtg
     3721 atcgttcatg gcagccagga caacaatgcg acggccactg ttctctggga caatgctttt
     3781 gcagagcctg gcagggtgcc atttgccgtg cctgacaaag tgctgtggcc acagctgtgt
     3841 gaggcgctca acatgaaatt caaggccgaa gtgcagagca accggggcct gaccaaggag
     3901 aacctcgtgt tcctggcgca gaaactgttc aacaacagca gcagccacct ggaggactac
     3961 agtggcctgt ctgtgtcctg gtcccagttc aacagggaga atttaccagg acggaattac
     4021 actttctggc aatggtttga cggtgtgatg gaagtgttaa aaaaacatct caagcctcat
     4081 tggaatgatg gggccatttt ggggtttgta aacaagcaac aggcccatga cctactcatt
     4141 aacaagccag atgggacctt cctcctgaga ttcagtgact cagaaattgg cggcatcacc
     4201 attgcttgga agtttgattc tcaggaaaga atgttttgga atctgatgcc ttttaccacc
     4261 agagacttct ccattcggtc cctagccgac cgcttgggag acttgaatta ccttatctac
     4321 gtgtttcctg atcggccaaa agatgaagta tactccaaat actacacacc agttccctgc
     4381 gagtctgcta ctgctaaagc tgttgatgga tacgtgaagc cacagatcaa gcaagtggtc
     4441 cctgagtttg tgaacgcatc tgcagatgcc gggggcggca gcgccacgta catggaccag
     4501 gccccctccc cagctgtgtg tccccaggct cactataaca tgtacccaca gaaccctgac
     4561 tcagtccttg acaccgatgg ggacttcgat ctggaggaca caatggacgt agcgcggcgt
     4621 gtggaggagc tcctgggccg gccaatggac agtcagtgga tcccgcacgc acaatcggga
     4681 tccgactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac
     4741 gatgacaagt agcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag
     4801 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     4861 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     4921 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     4981 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     5041 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     5101 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     5161 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     5221 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     5281 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc
     5341 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag
     5401 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc
     5461 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc
     5521 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac
     5581 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc
     5641 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc
     5701 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag
     5761 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg
     5821 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc
     5881 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg
     5941 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc
     6001 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc
     6061 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
     6121 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     6181 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     6241 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     6301 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     6361 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     6421 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     6481 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     6541 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     6601 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     6661 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     6721 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6781 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6841 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6901 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6961 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     7021 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     7081 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     7141 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7201 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7261 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     7321 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7381 aaagggggga ctggaagggc taattcactc ccaacgaaga taagatctgc tttttgcttg
     7441 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7501 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7561 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7621 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     7681 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7741 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7801 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     7861 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7921 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     7981 ttttttggag gcctagactt ttgcagagac caaattcgta atcatgtcat agctgtttcc
     8041 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8101 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8161 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8221 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8281 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8341 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8401 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8461 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8521 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8581 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8641 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8701 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8761 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8821 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     8881 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8941 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9001 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9061 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9121 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9181 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9241 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9301 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9361 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9421 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9481 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9541 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9601 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9661 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9721 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9781 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9841 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9901 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9961 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10021 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10081 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10141 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
    10201 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
    10261 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
    10321 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
    10381 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
    10441 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
    10501 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    10561 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
    10621 acgttgtaaa acgacggcca gtgccaagct g
//
