LOCUS       V039020                 9104 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039020
VERSION     V039020
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3936
                     /label="STK39(NM_013233)"
                     /note="STK39(NM_013233)"
                     /gene="STK39"
     CDS             3943..3969
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3973..3999
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4003..4029
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4074..4285
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4298..4566
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             4593..5192
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5199..5787
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5861..6094
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6166..6287
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6306..6441
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(6479..6495)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6503..6519
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6527..6557)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6572..6593
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6881..7469)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7640..8500)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8501..8605)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9079..9095
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggcggag ccgagcggct cgcccgtgca cgtccagctt
     2341 ccccagcagg cggccccggt gacagcggcg gcggcggcgg ccccggcggc cgcgacagca
     2401 gcgccggccc cggcagctcc cgcggccccg gccccggccc cggccccggc ggcacaggct
     2461 gtcggctggc ccatctgcag ggacgcgtac gagctgcagg aggttatcgg cagtggagct
     2521 actgctgtgg ttcaggcagc cctatgcaaa cccaggcaag aacgtgtagc aataaaacgg
     2581 atcaacttgg aaaaatgcca gaccagtatg gatgaactat taaaagaaat tcaagccatg
     2641 agtcagtgca gccatcccaa cgtagtgacc tattacacct cttttgtggt caaagatgaa
     2701 ctttggctgg tcatgaaatt actaagtgga ggttcaatgt tggatatcat aaaatacatt
     2761 gtcaaccgag gagaacacaa gaatggagtt ctggaagagg caataatagc aacaattctt
     2821 aaagaggttt tggaaggctt agactatcta cacagaaacg gtcagattca cagggatttg
     2881 aaagctggta atattcttct gggtgaggat ggttcagtac aaatagcaga ttttggggta
     2941 agtgcgttcc tagcaacagg gggtgatgtt acccgaaata aagtaagaaa aacattcgtt
     3001 ggcaccccat gttggatggc tcctgaagtc atggaacagg tgagaggcta tgacttcaag
     3061 gctgacatgt ggagttttgg aataactgcc attgaattag caacaggagc agcgccttat
     3121 cacaaatatc ctcccatgaa agtgttaatg ttgactttgc aaaatgatcc acccactttg
     3181 gaaacagggg tagaggataa agaaatgatg aaaaagtacg gcaagtcctt tagaaaatta
     3241 ctttcactgt gtcttcagaa agatccttcc aaaaggccca cagcagcaga acttttaaaa
     3301 tgcaaattct tccagaaagc caagaacaga gagtacctga ttgagaagct gcttacaaga
     3361 acaccagaca tagcccaaag agccaaaaag gtaagaagag ttcctgggtc aagtggtcac
     3421 cttcataaaa ccgaagacgg ggactgggag tggagtgacg acgagatgga tgagaagagc
     3481 gaagaaggga aagcagcttt ttctcaggaa aagtcacgaa gagtaaaaga agaaaatcca
     3541 gagattgcag tgagtgccag caccatcccc gaacaaatac agtccctctc tgtgcacgac
     3601 tctcagggcc cacccaatgc taatgaagac tacagagaag cttcttcttg tgccgtgaac
     3661 ctcgttttga gattaagaaa ctccagaaag gaacttaatg acatacgatt tgagtttact
     3721 ccaggaagag atacagcaga tggtgtatct caggagctct tctctgctgg cttggtggat
     3781 ggtcacgatg tagttatagt ggctgctaat ttacagaaga ttgtagatga tcccaaagct
     3841 ttaaaaacat tgacatttaa gttggcttct ggctgtgatg ggtcggagat tcctgatgaa
     3901 gtgaagctga ttgggtttgc tcagttgagt gtcagcggat cctacccata cgacgtacct
     3961 gattatgcaa cttatcctta tgatgtacct gattatgcta gctatcctta tgatgtccca
     4021 gactacgcat aggcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga
     4081 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc
     4141 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     4201 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     4261 caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg
     4321 cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc
     4381 cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag
     4441 accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct
     4501 ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta
     4561 cagatccaag ctgtgaccgg cgcctacgct agatgaccga gtacaagccc acggtgcgcc
     4621 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact
     4681 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc
     4741 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     4801 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     4861 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     4921 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     4981 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     5041 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     5101 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     5161 cctggtgcat gacccgcaag cccggtgcct gagtcgacaa tcaacctctg gattacaaaa
     5221 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     5281 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     5341 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     5401 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     5461 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     5521 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     5581 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     5641 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     5701 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     5761 ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca
     5821 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     5881 caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     5941 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6001 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6061 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     6121 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     6181 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6241 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     6301 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6361 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6421 gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt
     6481 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6541 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6601 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6661 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6721 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6781 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6841 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6901 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6961 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7021 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7081 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7141 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7201 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7261 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7321 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7381 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7441 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7501 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7561 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7621 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7681 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7741 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7801 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7861 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7921 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7981 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8041 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8101 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8161 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8221 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8281 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8341 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8401 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8461 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8521 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8581 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     8641 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     8701 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     8761 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     8821 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     8881 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     8941 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     9001 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     9061 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
