LOCUS       V021636                10413 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021636
VERSION     V021636
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2301..5267
                     /label="Scart1(NM_172909)"
                     /note="Scart1(NM_172909)"
                     /gene="Scart1"
     CDS             5274..5339
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5383..5594
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5607..5875
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5902..6501
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6508..7096
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7170..7403
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7475..7596
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7615..7750
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7788..7804)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7812..7828
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7836..7866)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7881..7902
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8190..8778)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8949..9809)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9810..9914)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10388..10404
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta
     2281 gagctagcga attcgccacc atgaggacaa cgctctggcc tctgagttta gggtctcttc
     2341 tcactctcgg ggcagctctc actggcacat catctgaagg tgaccaaggc aatctgaggc
     2401 tggcacacag acagagcaca tgtgacggag tggtgctggt tcgacaccaa ggggaatggg
     2461 gtcacctgtg caacctggag tggacactgg cagaggcctc tgtggtgtgt agacagctgg
     2521 gctgtggccc tgctgtgggt gctcccaaat atgtacccct gcctggagag atggtccggc
     2581 cctggctgca caatgtatcc tgttggggca atgagtcctc tctctgggag tgtagccttg
     2641 gggcatggag tcagcgtgct tgcccccatg agtgggtggt ggtggctctc tgtgcaaatg
     2701 gcaccttccg ggagatccgg ctggtgaagg gccacagtcc ctgtgcagga ctccctgaga
     2761 tcatgaatgt gaatggggtg gaccgcctct gtggcctgca tgtggaggag gccacagtgt
     2821 tttgccgtga gctgcagtgt ggccatgcac tccaggcccc ccgtcaagat gtgggcgtca
     2881 gtaagtacat gacctgcaag ggcaccgagt ccaccatccg caactgcaga ctcaacaaca
     2941 agctacgcgg tggctgcaac ctgctgctgg atgcagaagt catctgctca ggacacgtgg
     3001 aggcccggct agtgggtgga gaacattcct gtgctggacg cctggaggtt cttcggggac
     3061 tgacctgggg taccatctgc catgctgatc tggacttgcc cacagcccat gtggtgtgcc
     3121 gggagttagg gtgtggtata gctgtgtcta cacttggggg tgctcagttt ggccagggct
     3181 caggacctgt gtggctagaa gccttccgct gtgtggggaa tgaatcgctg ctattccact
     3241 gcccccggga gcccggtcac cactgtggcc acgagcagga tgctgcactc acctgctcag
     3301 agttccggct ggtcaatggc agcagtgact gtgaaggccg tgtggaactc caagtgcaag
     3361 ggacctgggc acctgtctgt gctgccaact gggacctagc agatgccatg gttctctgtc
     3421 accaactcaa ctgtggcaat gcagtatctg tacctccagg aggacatttt ggtgatgggg
     3481 atgatctcat ctggccagac atatttcatt gtgtggggac agagcctcat ttgcttcact
     3541 gtgcagcaag caccctgggg gcccatggtt gtgtcctggg gaatacagct tctaccatct
     3601 gttcaggact ccaagacatc ctgcggctga gagatggaca gagctactgt gatggccgtg
     3661 tagagatttc cctggatggt tcatggggcc gcgtattgga tgacgcctgg gacctgcatg
     3721 gcgccagcgt ggtgtgccgg cagctagggt gtggagaggc acagcgagcc tatgatgctc
     3781 cagctcctat tttcaggaga gtccctgtgg ggctgagcca ggtgcgctgc ctgggctctg
     3841 agactcgcct ggtgcattgc aatgtttcta cgtccctgct ggtgcctgct gggaccttgc
     3901 gtgacgcagg agtggtgtgc tccgggagcc tgcgcgtgag gctggctgcg ggcccaggtc
     3961 gatgtgctgg gcgtgtggag ttattctatc agggctcctg gggcactgtg tgtgatgatg
     4021 cctgggacct acaggatgca caagtggtct gtaggcagct ggactgtggc cacgccctca
     4081 gtgctccccg gaatgctcac tttggggcgg ggactgggca catctggatg gatgagctgc
     4141 actgcttggg aaatgagtct tcgttgtgga agtgccagtc aggaagctgg ggccaacatg
     4201 actgcagaca caaggaggat gctggagtgt tctgctcaga atctgtggct ctgaggcttc
     4261 gaggtggcac tagtggctgc gttggatggc tggacgtgct ctacaatggg tcctggggtg
     4321 ctgtgtgcag caatgcactc agagacatct ccttgagcat catctgcaga cagctgggat
     4381 gtggggacca gggctggctg gagaacaggc cttcttctgg agctggcctg ggaatctcct
     4441 gggtggataa tatcaagtgt cgaaatctac acaatgccac cctgtggcaa tgcccttctg
     4501 ccccatggaa cccgcactcc tgcacccagg aggaggaggt ctggattaac tgtgaaggaa
     4561 caccacagat agctctccag gaccctgcac agaccccaaa ctgctcttct ccatttggct
     4621 gcctagagaa cagtgttctg cgtgtgcttg ggggtgagga tggctgttcc gggcgtgtgg
     4681 agctctggca cgcaggctca tggggcacag tgtgtgacga ctcctgggac ctggtggatg
     4741 ccgaggttgt gtgccggcaa ctgggctgcg gccaggcctt ggctgcttta ggagaggctt
     4801 cctttggccc tggtgtgggg cctgtgtggc tggatgaggt cgagtgcctt ggcagtgagg
     4861 tgaccctgga ggcctgccag gctgagccct ggggacatgg agactgcacg cacaaggagg
     4921 atgcaggtgt tcgctgtgcg ggtgtctcca cgaccactgc attgtctcct tccctgaccc
     4981 ctccatcggt tgccgaggtc tggaaactgt cggagattgc ctgcttggtg cttggctgcc
     5041 ttctgggcat tgtcttcctg gttatgattg ctcagtggtg ccacatcaga gatgctagca
     5101 tgggtgctgg catgttgggg catctacagt cagaaggtat ctatgaggac attgaagttg
     5161 accctgtgcc aaagaaggag gagaacacag tatccaggct actgatgctg gaagagggtt
     5221 atgatgatgc tgaagagcct gaggatcagc ccacagagaa tgacgaggga tccgactaca
     5281 aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt
     5341 agcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc
     5401 ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt
     5461 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     5521 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     5581 ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc
     5641 ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg
     5701 cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt
     5761 gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc
     5821 tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc
     5881 tgtgaccggc gcctacgcta gatgaccgag tacaagccca cggtgcgcct cgccacccgc
     5941 gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     6001 cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     6061 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     6121 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     6181 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     6241 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     6301 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6361 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     6421 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     6481 acccgcaagc ccggtgcctg agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     6541 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6601 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6661 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6721 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     6781 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6841 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6901 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     6961 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7021 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7081 tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct
     7141 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata
     7201 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7261 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7321 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7381 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     7441 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     7501 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7561 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     7621 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7681 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7741 tttggaggcc tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt
     7801 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     7861 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     7921 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     7981 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8041 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8101 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8161 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8221 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8281 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8341 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8401 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8461 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8521 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8581 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     8641 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8701 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8761 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8821 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8881 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8941 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9001 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9061 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9121 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9181 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9241 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9301 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9361 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9421 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9481 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9541 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9601 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9661 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9721 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9781 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9841 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9901 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     9961 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
    10021 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
    10081 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    10141 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
    10201 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
    10261 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    10321 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    10381 cgacgttgta aaacgacggc cagtgccaag ctg
//
