LOCUS       V037508                10732 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037508
VERSION     V037508
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4758
                     /label="Slc9a2(NM_001033289)"
                     /note="Slc9a2(NM_001033289)"
                     /gene="Slc9a2"
     CDS             4765..4830
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4874..5085
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5098..5366
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5395..5404
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5425..6087
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6157..6210
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6211..6810
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6811..7399
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7473..7706
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7778..7899
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7939..8074
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8107..8123)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8131..8147
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8155..8185)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8200..8221
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8509..9097)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9268..10128)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10129..10233)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10707..10723
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gcccccgggg caccgcgcac
     2341 agcgtgcggg cgccactgtc ttggctgctg ctgctgctgc tgtctctgca ggtggcagtg
     2401 ccggcgggcg cgctggccga gaccctgctg gacgcaccca gggccagggg tgccagctcc
     2461 aacccgccaa gtcctgccag tgtggtggcc ccgggaacga cgccgttcga ggaaagtcgg
     2521 ttgcccgtgt tcaccctgga ttacccccac gtgcaaatcc ctttcgagat cactttgtgg
     2581 atcctactgg cctccttggc caagattggt tttcatctct atcacaagtt gcccacaatc
     2641 gtgccggaga gctgccttct cataatggtc gggcttctac tgggtgggat tatcttcggt
     2701 gttgatgaga agtctccccc ggcaatgaag acagatgtgt ttttcctata cctcctgcca
     2761 cccatcgttc tggatgctgg ctacttcatg ccaactcgcc ccttctttga gaacttcggc
     2821 accattttct ggtatgctgt ggtaggcaca ctgtggaatt ccattggcat tggggtgtct
     2881 ttgtttggca tctgccagat tgaggcgttt ggcctcagtg acatcaccct gctccagaac
     2941 ctgctcttcg gcagcctgat ttcagctgtg gaccccgtgg ctgtgcttgc tgtctttgag
     3001 aacattcacg tcaatgaaca gctctatatc ctggtttttg gcgagtctct gctcaacgat
     3061 gcagtcacag tggtcctgta caacttgttc aagtccttct gccagatgaa aaccatccag
     3121 accgtagacg tgtttgctgg catcgctaac ttcttcgtcg tgggtattgg cggggtgctg
     3181 attggcatcc tcctgggatt catagcagcg tttaccaccc gtttcacaca caacatccgg
     3241 gtcattgagc cactctttgt cttcttgtac agctacttgt cctacatcac tgctgaaatg
     3301 ttccaccttt caggcatcat ggcgatcaca gcgtgtgcca tgacaatgaa taagtacgtg
     3361 gaagagaacg tgtctcagaa gtcctacaca accatcaagt acttcatgaa gatgctgagc
     3421 agcgtgagcg agaccctgat cttcatcttc atgggcgtgt ccactgttgg gaagaaccac
     3481 gagtggaact gggctttcgt ctgctttacc ctggccttct gcctgatctg gcgagcactg
     3541 ggcgtctttg tcctgactca ggtgattaac tggttccgga ccatcccgct aaccttcaag
     3601 gaccagttca tcattgccta cggagggctc cgtggcgcca tctgctttgc actggtgttt
     3661 ctcctccctg ctactgtgtt tccccgaaag aagctgttca tcacggctgc tattgtcgtt
     3721 atattcttca ctgtcttcat cctgggtata accatccggc cactggtgga atttcttgat
     3781 gttaagagat ccaataagaa gcagcaagct gtcagtgaag agatccactg tcggtttttt
     3841 gatcatgtga agactgggat tgaagatgtg tgtggacact ggggtcacaa cttctggaga
     3901 gacaagttta agaagtttga tgacaaatac cttcggaagc ttctgattcg ggaaaaccaa
     3961 cccaagtcca gtattgtgtc cttatataaa aagcttgaga taaagcatgc cattgaaatg
     4021 gcagagacag ggatgataag cactgtcccc tcatttgcat ctctcaatga ctgccgtgaa
     4081 gagaaaataa ggaagctcac gccaggcgaa atggatgaaa ttagagagat attatcacgg
     4141 aatctctatc aaatccgtca gcggaccttg tcctataaca gacacaacct gacggcagac
     4201 acaagcgaga ggcaagccaa ggagatcctg attcgcaggc ggcacagtct tcgggaaagt
     4261 ctcaggaaag acaacagctc aaaccgcgag cgcagggctt ccacttcaac ctcccgatat
     4321 ttatccttac ctaagaacac aaagcttcca gagaagctgc agaagaagaa gaaggtttca
     4381 aatgcagatg gaaatagcag cgactcggac ccagatgttg gaaccactgt gctcaatttg
     4441 cagcccagga ccaggcgctt cttgccagat cagttctcaa agaaagcctc ccaggcctac
     4501 aaaatggaat ggaagaacga ggtggatgtg ggttctgtgc gagtgccccc cagcgtctct
     4561 ccagcgcccc gcagtaaaga cgggggcacc caaacaccgg ggttgctgag gcagcccctg
     4621 ctctccaaag accaacggtt tggccggggt agggaagaca gtttgactga agacgttcca
     4681 cccaagcccc caccaaggtt ggtgcgacga gcgtcagaac ccgggaacag gaaaggccgc
     4741 cttggcaatg aaaagccagg atccgactac aaagaccatg acggtgatta taaagatcat
     4801 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc
     4861 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     4921 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     4981 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     5041 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     5101 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     5161 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     5221 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     5281 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     5341 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc
     5401 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc
     5461 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc
     5521 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc
     5581 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc
     5641 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac
     5701 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac
     5761 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc
     5821 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc
     5881 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac
     5941 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc
     6001 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac
     6061 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg
     6121 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca
     6181 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc
     6241 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     6301 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     6361 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     6421 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     6481 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     6541 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     6601 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     6661 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     6721 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     6781 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa
     6841 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6901 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6961 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7021 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7081 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7141 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7201 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7261 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7321 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     7381 ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga
     7441 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     7501 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     7561 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7621 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7681 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     7741 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     7801 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7861 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     7921 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7981 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     8041 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt
     8101 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8161 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8221 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8281 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8341 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8401 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8461 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8521 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8581 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8641 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8701 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8761 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8821 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8881 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8941 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9001 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9061 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9121 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9181 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9241 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9301 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9361 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9421 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9481 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9541 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9601 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9661 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9721 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9781 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9841 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9901 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9961 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10021 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10081 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10141 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10201 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    10261 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    10321 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    10381 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    10441 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    10501 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    10561 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    10621 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    10681 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
