LOCUS       V026677                10801 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026677
VERSION     V026677
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4827
                     /label="Slitrk4(NM_178740)"
                     /note="Slitrk4(NM_178740)"
                     /gene="Slitrk4"
     CDS             4834..4899
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4943..5154
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5167..5435
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5464..5473
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5494..6156
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6226..6279
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6280..6879
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6880..7468
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7542..7775
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7847..7968
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8008..8143
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8176..8192)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8200..8216
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8224..8254)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8269..8290
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8578..9166)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9337..10197)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10198..10302)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10776..10792
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt ttctttggct ctttctgatt
     2341 gtgtcagccc tgatttcttc tacaaatgca gattctgaca tatcagtgga aatttgcaat
     2401 gtgtgttcct gcgtgtcagt agagaatgtg ctctatgtca actgtgagaa ggtttcagtc
     2461 tacaggccaa accagctgaa accaccttgg tctaatttct atcatctcaa tttccaaaac
     2521 aattttttaa atatcctcta tccaaataca ttcgtgaatt tttctcatgc agtatctctg
     2581 catctgggaa ataataagct gcagaacatt gagggaggag cctttctcgg gctcagtgca
     2641 ttaaagcagt tgcacttgaa caacaatgaa ttaaagattc tccgagctga cactttcctt
     2701 ggcatagaga acttggagta tctccaggct gactacaatt taatcaagta tattgaacga
     2761 ggagccttca ataagctcca taaactgaaa gttctcattc ttaatgacaa tctgatttca
     2821 ttccttcctg ataatatttt tcgatttgca tctttaaccc atttggatat acgaggaaac
     2881 agaatccaga aacttcctta tattggagtt ctagaacaca ttggccgggt tgttgagttg
     2941 cagctggaag ataatccttg gaactgtagc tgtgatttgt tgcctctaaa ggcttggctg
     3001 gagaatatgc catataacat ttacatagga gaagctattt gtgaaactcc cagtgactta
     3061 tatggaaggc ttttaaagga aaccaacaag caagaactat gccccatggg tacaggcagt
     3121 gactttgacg tacgcattct gcctccatct cagcaggaga atggctttac tactcccaat
     3181 ggtcacacta ctcagacaac cttgcacaga ttagtgacaa aaccacccaa aacaacaaat
     3241 ccttccaaaa tctctggaat tgtggcaggc aaagccctat ccaacagaaa tctcagtcag
     3301 attgtgtcat atcaaacaag agtgcctcct cttacacctt gtccagtacc ttgcttttgc
     3361 aaaacacatc cttctgattt gggattgagt gtgaattgcc aagagaaaaa tatacagtcc
     3421 atgtctgaat tgacaccaaa acctttgaat gccaagaagt tgcatgtcaa tggtaataac
     3481 atcaaagatg tggacatttc agacttcact gagtttgagg ggctggattt gctccattta
     3541 ggcagcaatc agattacgtt gatcaaagga gaagtattcc acaatcttac taatctgcgc
     3601 aggctttatc tcaatggcaa tcagattgaa aggctatacc ctgaaatatt ttcaggactt
     3661 cataacctgc agtatcttta tttggagtac aacttgatta aggaaatttt agcaggcacc
     3721 tttgactcca tgccaaattt gcagctactg tacttgaaca ataatctctt aaaaagcctg
     3781 ccagtttaca tcttttctgg agcaccactt gctagactga acctgagaaa caacaaattc
     3841 atgtacctac ctgtcagcgg agtacttgat cagttgcagt ctcttacaca aattgactta
     3901 gagggcaacc cttgggactg cacctgcgac ttggtagcat taaagttgtg gctggagaag
     3961 ttaaatgatg ggattgttgt gaaagaactg aaatgtgaga ctcctgttca gtttgccaac
     4021 atcgaattga agtccctcaa aaatgaaatc ttatgcccta aactcttaaa caagccatca
     4081 gcaacattca caagccctgc gcctgcaatt accttcacca ctccattggg tcctattcga
     4141 agtcctcctg ggggcccagt gccattatct attttaatct taagtattct ggtggttctc
     4201 attttaactg tctttgttgc attttgcctt cttgtttttg tgctgagacg taacaagaaa
     4261 cccacagtga agcatgaagg tctggggaat tctgagtgtg gctccatgca gctgcaacta
     4321 aggaaacatg accacaaaac caataaaaaa gatggactga gcacagaagc attcattcca
     4381 caaaccatcg aacagatgag caaaagtcac acctgtggcc tgaaagagtc agaaactggg
     4441 ttcatgtttt ctgatcctcc gggacagaag gtaatgatga gaaatgctgc tgacaaggac
     4501 aaagatttat tgcatgtgga taccaggaaa agactgagca caattgacga gctggacgaa
     4561 ctcttcccta gcagagattc caatgtgttc attcaaaatt ttcttgaaag caaaaaggag
     4621 tataacagca ttggtgtcag tggctttgag attcgctatc ctgaaaaaca acaagataaa
     4681 aaaaacaaga agtcactgat aggtggtaac cacagtaaaa ttgttgttga acaaaggaag
     4741 agtgagtatt ttgaactgaa ggcaaaactt cagagttccc ccgactacct acaggtcctt
     4801 gaagagcaaa cagctttgaa caagatagga tccgactaca aagaccatga cggtgattat
     4861 aaagatcatg acatcgacta caaggatgac gatgacaagt agcggccgcg aaggatctgc
     4921 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     4981 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     5041 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     5101 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     5161 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     5221 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     5281 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     5341 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     5401 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta
     5461 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc
     5521 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag
     5581 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac
     5641 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac
     5701 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag
     5761 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg
     5821 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac
     5881 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg
     5941 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg
     6001 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc
     6061 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag
     6121 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat
     6181 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt
     6241 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg
     6301 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     6361 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     6421 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     6481 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     6541 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     6601 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     6661 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     6721 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     6781 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     6841 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa
     6901 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6961 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7021 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7081 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7141 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7201 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7261 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     7321 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7381 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7441 cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc
     7501 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     7561 ccaacgaaga taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     7621 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7681 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7741 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     7801 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     7861 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7921 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     7981 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     8041 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     8101 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
     8161 caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8221 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8281 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8341 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8401 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8461 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8521 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8581 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8641 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8701 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8761 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8821 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8881 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8941 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9001 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9061 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9121 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9181 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9241 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9301 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9361 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9421 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9481 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9541 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9601 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9661 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9721 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9781 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9841 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9901 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9961 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10021 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10081 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10141 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10201 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10261 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10321 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    10381 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    10441 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    10501 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    10561 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    10621 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    10681 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    10741 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
    10801 g
//
