LOCUS V047378 8404 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V047378 VERSION V047378 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..3258 /label="Sox2(NM_011443)" /note="Sox2(NM_011443)" /gene="Sox2" CDS 3265..3330 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 3374..3585 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3598..3866 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 3893..4492 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 4499..5087 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5161..5394 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 5466..5587 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 5606..5741 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(5779..5795) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5803..5819 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5827..5857) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5872..5893 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6181..6769) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6940..7800) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7801..7905) /gene="bla" /label="AmpR promoter" primer_bind 8379..8395 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catgtataac atgatggaga cggagctgaa gccgccgggc 2341 ccgcagcaag cttcgggggg cggcggcgga ggaggcaacg ccacggcggc ggcgaccggc 2401 ggcaaccaga agaacagccc ggaccgcgtc aagaggccca tgaacgcctt catggtatgg 2461 tcccgggggc agcggcgtaa gatggcccag gagaacccca agatgcacaa ctcggagatc 2521 agcaagcgcc tgggcgcgga gtggaaactt ttgtccgaga ccgagaagcg gccgttcatc 2581 gacgaggcca agcggctgcg cgctctgcac atgaaggagc acccggatta taaataccgg 2641 ccgcggcgga aaaccaagac gctcatgaag aaggataagt acacgcttcc cggaggcttg 2701 ctggcccccg gcgggaacag catggcgagc ggggttgggg tgggcgccgg cctgggtgcg 2761 ggcgtgaacc agcgcatgga cagctacgcg cacatgaacg gctggagcaa cggcagctac 2821 agcatgatgc aggagcagct gggctacccg cagcacccgg gcctcaacgc tcacggcgcg 2881 gcacagatgc aaccgatgca ccgctacgac gtcagcgccc tgcagtacaa ctccatgacc 2941 agctcgcaga cctacatgaa cggctcgccc acctacagca tgtcctactc gcagcagggc 3001 acccccggta tggcgctggg ctccatgggc tctgtggtca agtccgaggc cagctccagc 3061 ccccccgtgg ttacctcttc ctcccactcc agggcgccct gccaggccgg ggacctccgg 3121 gacatgatca gcatgtacct ccccggcgcc gaggtgccgg agcccgctgc gcccagtaga 3181 ctgcacatgg cccagcacta ccagagcggc ccggtgcccg gcacggccat taacggcaca 3241 ctgcccctgt cgcacatggg atccgactac aaagaccatg acggtgatta taaagatcat 3301 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc 3361 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 3421 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 3481 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 3541 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 3601 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 3661 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 3721 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 3781 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 3841 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agatgaccga 3901 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct 3961 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat 4021 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 4081 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 4141 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 4201 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 4261 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 4321 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 4381 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 4441 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagtcgacaa 4501 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 4561 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 4621 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 4681 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 4741 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 4801 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 4861 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc 4921 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 4981 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 5041 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa 5101 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 5161 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc 5221 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 5281 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 5341 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 5401 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 5461 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 5521 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 5581 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc 5641 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 5701 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg 5761 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 5821 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 5881 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 5941 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 6001 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 6061 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 6121 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 6181 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 6241 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 6301 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 6361 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 6421 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 6481 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 6541 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 6601 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 6661 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 6721 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 6781 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 6841 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 6901 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 6961 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 7021 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 7081 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 7141 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 7201 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 7261 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 7321 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 7381 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 7441 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 7501 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 7561 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 7621 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 7681 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 7741 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 7801 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 7861 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 7921 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 7981 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 8041 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 8101 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 8161 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 8221 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 8281 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 8341 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 8401 gctg //