LOCUS       V045969                11692 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045969
VERSION     V045969
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5718
                     /label="Srebf1(NM_011480)"
                     /note="Srebf1(NM_011480)"
                     /gene="Srebf1"
     CDS             5725..5790
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5834..6045
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6058..6326
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6355..6364
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6385..7047
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7117..7170
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7171..7770
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7771..8359
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8433..8666
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8738..8859
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8899..9034
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9067..9083)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9091..9107
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9115..9145)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9160..9181
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9469..10057)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10228..11088)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11089..11193)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11667..11683
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg acgagctggc cttcggtgag
     2341 gcggctctgg aacagacact ggccgagatg tgcgaactgg acacagcggt tttgaacgac
     2401 atcgaagaca tgctccagct catcaacaac caagacagtg acttcccggg cctgtttgac
     2461 gccccctatg ctgggggtga gacaggggac acaggcccca gcagcccagg tgccaactct
     2521 cctgagagct tctcttctgc ttctctggcc tcctctctgg aagccttcct gggaggaccc
     2581 aaggtgacac ctgcaccctt gtcccctcca ccatcggcac ccgctgcttt aaagatgtac
     2641 ccgtccgtgt cccccttttc ccctgggcct gggatcaaag aggagccagt gccactcacc
     2701 atcctacagc ctgcagcgcc acagccgtca ccggggaccc tcctgcctcc gagcttcccc
     2761 gcaccacccg tacagctcag ccctgcgccc gtgctgggtt actcgagcct gccttcaggc
     2821 ttctcaggga cccttccagg aaacactcag cagccaccat ctagcctgcc gctggcccct
     2881 gcaccaggag tcttgcccac ccctgccctg cacacccagg tccaaagctt ggcctcccag
     2941 cagccgctgc cagcctcagc agcccctaga acaaacactg tgacctcaca ggtccagcag
     3001 gtcccagttg tactgcagcc acacttcatc aaggcagact cactgctgct gacagctgtg
     3061 aagacagatg caggagccac cgtgaagact gcaggcatca gcaccctggc tcctggcaca
     3121 gccgtgcagg caggtcccct gcagaccctg gtgagtggag ggaccatctt ggccacagta
     3181 cctttggttg tggacacaga caaactgccc atccaccgac tcgcagctgg cagcaaggcc
     3241 ctaggctcag ctcagagccg tggtgagaag cgcacagccc acaatgccat tgagaagcgc
     3301 taccggtctt ctatcaatga caagattgtg gagctcaaag acctggtggt gggcactgaa
     3361 gcaaagctga ataaatctgc tgtcttgcgc aaggccatcg actacatccg cttcttgcag
     3421 cacagcaacc agaagctcaa gcaggagaac ctgaccctac gaagtgcaca caaaagcaaa
     3481 tcactgaagg acctggtgtc agcttgtggc agtggaggag gcacagatgt gtctatggag
     3541 ggcatgaaac ccgaagtggt ggagacgctt acccctccac cctcagacgc cggctcaccc
     3601 tcccagagta gccccttgtc ttttggcagc agagctagca gcagtggtgg tagtgactct
     3661 gagcccgaca gtccagcctt tgaggatagc caggtcaaag cccagcggct gccttcacac
     3721 agccgaggca tgctggaccg ctcccgcctg gccctgtgtg tactggcctt tctgtgtctg
     3781 acctgcaatc ctttggcctc gcttttcggc tggggcattc tcactccctc tgatgctacg
     3841 ggtacacacc gtagttctgg gcgcagcatg ctggaggcag agagcagaga tggctctaat
     3901 tggacccagt ggttgctgcc acccctagtc tggctggcca atggactact agtgttggcc
     3961 tgcttggctc ttctctttgt ctatggggaa cctgtgacta ggccacactc tggcccggct
     4021 gtacacttct ggagacatcg caaacaagct gacctggatt tggcccgggg agatttcccc
     4081 caggctgctc aacagctgtg gctggccctg caagcgctgg gccggcccct gcccacctca
     4141 aacctggatc tggcctgcag tctgctttgg aacctcatcc gccacctgct ccagcgtctc
     4201 tgggtgggcc gctggctggc aggccaggcc gggggcctgc tgagggaccg tgggctgagg
     4261 aaggatgccc gtgccagtgc ccgggatgcg gctgttgtct accataagct gcaccagctg
     4321 catgccatgg gcaagtacac aggaggacat cttgctgctt ctaacctggc actaagtgcc
     4381 ctcaacctgg ctgagtgcgc aggagatgct atctccatgg caacactggc agagatctat
     4441 gtggcagcgg ccctgagggt caaaaccagc ctcccaagag ccctgcactt cttgacacgt
     4501 ttcttcctga gcagcgcccg ccaggcctgc ctagcacaga gcggctcggt gcctcttgcc
     4561 atgcagtggc tctgccaccc tgtaggtcac cgtttctttg tggacgggga ctgggccgtg
     4621 cacggtgccc ccccggagag cctgtacagc gtggctggga acccagtgga tccgctggcc
     4681 caggtgaccc ggctattccg tgaacatctc ctagagcgag cgttgaactg tattgctcag
     4741 cccagcccag gggcagctga cggagacagg gagttctcag atgcccttgg atatctgcag
     4801 ttgctaaata gctgttctga tgctgccggg gctcctgctt gcagtttctc tgtcagctcc
     4861 agcatggctg ccaccactgg cccagaccca gtggccaagt ggtgggcctc actgacagct
     4921 gtggtgatcc actggctgag gcgggatgaa gaggcagctg agcgcttgta cccactggta
     4981 gagcatatcc cccaggtgct gcaggacact gagagacccc tgcccagggc agctctgtac
     5041 tccttcaagg ctgcccgggc tctgctggac cacagaaagg tggaatctag cccagccagc
     5101 ctggccatct gtgagaaggc cagtgggtac ctgcgggaca gcttagcctc tacaccaact
     5161 ggcagttcca ttgacaaggc catgcagctg ctcctgtgtg atctacttct tgtggcccgt
     5221 accagtctgt ggcagcggca gcagtcacca gcttcagtcc aggtagctca cggtaccagc
     5281 aatggacccc aggcctctgc tctggagctg cgtggtttcc aacatgacct gagcagcctg
     5341 cggcggttgg cacagagctt ccggcctgct atgaggaggg tattcctaca tgaggccaca
     5401 gctcggctga tggcaggagc aagtcctgcc cggacacacc agctcctgga tcgcagtctg
     5461 aggaggaggg caggttccag tggcaaagga ggcactacag ctgagctgga gccacggccc
     5521 acatggcggg agcacaccga ggccctgctg ttggcatcct gctatctgcc ccctgccttc
     5581 ctgtcggctc ctgggcagcg aatgagcatg ctggccgagg cggcacgcac cgtagagaag
     5641 cttggcgatc accggctact gctggactgc cagcagatgc tcctgcgcct gggcggcgga
     5701 accaccgtca cttccagcgg atccgactac aaagaccatg acggtgatta taaagatcat
     5761 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc
     5821 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     5881 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     5941 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     6001 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     6061 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     6121 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     6181 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     6241 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     6301 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc
     6361 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc
     6421 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc
     6481 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc
     6541 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc
     6601 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac
     6661 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac
     6721 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc
     6781 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc
     6841 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac
     6901 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc
     6961 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac
     7021 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg
     7081 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca
     7141 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc
     7201 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     7261 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     7321 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     7381 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     7441 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     7501 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     7561 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     7621 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     7681 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     7741 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa
     7801 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7861 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7921 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7981 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     8041 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     8101 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     8161 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     8221 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     8281 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     8341 ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga
     8401 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     8461 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     8521 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     8581 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     8641 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     8701 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     8761 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8821 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     8881 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     8941 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9001 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt
     9061 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     9121 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     9181 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     9241 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     9301 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     9361 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     9421 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     9481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9661 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9841 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9901 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9961 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10021 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10081 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    10141 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    10201 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    10261 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    10321 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    10381 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    10441 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    10501 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    10561 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    10621 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    10681 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10741 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10801 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10861 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10921 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10981 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    11041 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    11101 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    11161 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    11221 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    11281 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    11341 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    11401 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    11461 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    11521 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    11581 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    11641 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
