LOCUS       V030408                10582 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030408
VERSION     V030408
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3018
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             3055..5421
                     /label="TAX1BP1(NM_006024)"
                     /note="TAX1BP1(NM_006024)"
                     /gene="TAX1BP1"
     CDS             5422..5448
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5452..5478
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5482..5508
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5552..5763
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5776..6044
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6071..6670
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6677..7265
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7339..7572
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7644..7765
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7784..7919
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7957..7973)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7981..7997
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8005..8035)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8050..8071
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8359..8947)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9118..9978)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9979..10083)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10557..10573
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     2341 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2401 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2461 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2521 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2581 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2641 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2701 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2761 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2821 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2881 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     2941 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3001 atggacgagc tgtacaaggg cggcggaggg agtggaggag ggggaagtgg atccatgaca
     3061 tcctttcaag aagtcccatt gcagacttcc aactttgccc atgtcatctt tcaaaatgtg
     3121 gccaagagtt accttcctaa tgcacacctg gaatgtcatt acaccttaac tccatatatt
     3181 catccacatc caaaagattg ggttggtata ttcaaggttg gatggagtac tgctcgtgat
     3241 tattacacgt ttttatggtc ccctatgcct gaacattatg tggaaggatc aacagtcaat
     3301 tgtgtactag cattccaagg atattacctt ccaaatgatg atggagaatt ttatcagttc
     3361 tgttacgtta cccataaggg tgaaattcgt ggagcaagta cacctttcca gtttcgagct
     3421 tcttctccag ttgaagagct gcttactatg gaagatgaag gaaattctga catgttagtg
     3481 gtgaccacaa aagcaggcct tcttgagttg aaaattgaga aaaccatgaa agaaaaagaa
     3541 gaactgttaa agttaattgc cgttctggaa aaagaaacag cacaacttcg agaacaagtt
     3601 gggagaatgg aaagagaact taaccatgag aaagaaagat gtgaccaact gcaagcagaa
     3661 caaaagggtc ttactgaagt aacacaaagc ttaaaaatgg aaaatgaaga gtttaagaag
     3721 aggttcagtg atgctacatc caaagcccat cagcttgagg aagatattgt gtcagtaaca
     3781 cataaagcaa ttgaaaaaga aaccgaatta gacagtttaa aggacaaact caagaaggca
     3841 caacatgaaa gagaacaact tgaatgtcag ttgaagacag agaaggatga aaaggaactt
     3901 tataaggtac atttgaagaa tacagaaata gaaaatacca agcttatgtc agaggtccag
     3961 actttaaaaa atttagatgg gaacaaagaa agcgtgatta ctcatttcaa agaagagatt
     4021 ggcaggctgc agttatgttt ggctgaaaag gaaaatctgc aaagaacttt cctgcttaca
     4081 acctcaagta aagaagatac ttgtttttta aaggagcaac ttcgtaaagc agaggaacag
     4141 gttcaggcaa ctcggcaaga agttgtcttt ctggctaaag aactcagtga tgctgtcaac
     4201 gtacgagaca gaacgatggc agacctgcat actgcacgct tggaaaacga gaaagtgaaa
     4261 aagcagttag ctgatgcagt ggcagaactt aaactaaatg ctatgaaaaa agatcaggac
     4321 aagactgata cactggaaca cgaactaaga agagaagttg aagatctgaa actccgtctt
     4381 cagatggctg cagaccatta taaagaaaaa tttaaggaat gccaaaggct ccaaaaacaa
     4441 ataaacaaac tttcagatca atcagctaat aataataatg tcttcacaaa gaaaacgggg
     4501 aatcagcaga aagtgaatga tgcttcagta aacacagacc cagccacttc tgcctctact
     4561 gtagatgtaa agccatcacc ttctgcagca gaggcagatt ttgacatagt aacaaagggg
     4621 caagtctgtg aaatgaccaa agaaattgct gacaaaacag aaaagtataa taaatgtaaa
     4681 caactcttgc aggatgagaa agcaaaatgc aataaatatg ctgatgaact tgcaaaaatg
     4741 gagctgaaat ggaaagaaca agtgaaaatt gctgaaaatg taaaacttga actagctgaa
     4801 gtacaggaca attataaaga acttaaaagg agtctagaaa atccagcaga aaggaaaatg
     4861 gaaggtcaga attcccagag tcctcaatgt ttcaaaacat gctcagagca aaatggttat
     4921 gttctcacat tgtcaaatgc acaaccagtt ctgcaatatg gtaatcctta tgcatctcag
     4981 gaaacaagag atggagcaga tggtgctttt tacccagatg aaatacaaag gccacctgtc
     5041 agagtcccct cttggggact ggaagacaat gttgtctgca gccagcctgc tcgaaacttt
     5101 agtcggcctg atggcttaga ggactctgag gatagcaaag aagatgagaa tgtgcctact
     5161 gctcctgatc ctccaagtca acatttacgt gggcatggga caggcttttg ctttgattcc
     5221 agctttgatg ttcacaagaa gtgtcccctc tgtgagttaa tgtttcctcc taactatgat
     5281 cagagcaaat ttgaagaaca tgttgaaagt cactggaagg tgtgcccgat gtgcagcgag
     5341 cagttccctc ctgactatga ccagcaggtg tttgaaaggc atgtgcagac ccattttgat
     5401 cagaatgttc taaattttga ctacccatac gacgtacctg attatgcaac ttatccttat
     5461 gatgtacctg attatgctag ctatccttat gatgtcccag actacgcata gcggccgcga
     5521 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     5581 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     5641 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     5701 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     5761 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     5821 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     5881 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     5941 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     6001 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     6061 cctacgctag atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     6121 ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     6181 cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     6241 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     6301 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     6361 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     6421 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     6481 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     6541 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     6601 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     6661 cggtgcctga gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6721 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     6781 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     6841 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     6901 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     6961 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7021 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     7081 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7141 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7201 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7261 cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     7321 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa gatctgcttt
     7381 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7441 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7501 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7561 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     7621 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     7681 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7741 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     7801 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7861 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7921 agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa
     7981 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8041 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8101 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8161 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8221 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8281 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8341 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8401 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8461 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8521 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8581 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8641 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8701 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8761 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     8821 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8881 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8941 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9001 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9061 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9121 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9181 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9241 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9301 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9361 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9421 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9481 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9541 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9601 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9661 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9721 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9781 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9841 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9901 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9961 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10021 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10081 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    10141 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
    10201 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    10261 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
    10321 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
    10381 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
    10441 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    10501 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    10561 aacgacggcc agtgccaagc tg
//
