LOCUS V036928 9067 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V036928 VERSION V036928 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" regulatory 2296..2305 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 2305..2394 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" CDS 2401..3978 /label="TBC1D19(NM_018317)" /note="TBC1D19(NM_018317)" /gene="TBC1D19" promoter 4037..4248 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4261..4529 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4556..5155 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5162..5750 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5824..6057 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6129..6250 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6269..6404 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6442..6458) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6466..6482 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6490..6520) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6535..6556 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6844..7432) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7603..8463) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8464..8568) /gene="bla" /label="AmpR promoter" primer_bind 9042..9058 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggaacaa aaactaatat cagaggaaga tttggaacag 2341 aaactaatca gtgaagaaga tttagagcaa aagttaattt ccgaggagga cttactcgag 2401 atgttgcagg aggagtcgga cctctctctc attattgccc agatagtcca aaagctcaag 2461 ggctccaatt tgtactctca gctggaacgg caggcctggg ccagtcttca gagacctgag 2521 attaaacttg aatcactgaa agaagatatt aaggaattct ttaaaatatc aggttgggag 2581 aagaaacttc agaatgctgt ttatagtgaa ctgagtgtgt ttcctttacc tagtcatcct 2641 gctgcacctc ctgaacatct taaagaacct ttggtataca tgaggaaagc acagggaagt 2701 tgggaaaaaa gaattttgaa gagtttaaat agtatgtgca ctgaactgag tatcccactg 2761 gcacgaaaga ggccagttgg agaacagaaa gaacttctta ataaatggaa tgaaatggga 2821 actgatgaac cagatttaag ccttttcaga cctgtttatg cacctaagga ttttcttgag 2881 gtattaatta atcttcgcaa cccaaattat gaaaacggtg attctcttag tttcaggact 2941 catttgggtt taattcaagt tccactgaaa gtaaaagaca tccctgaatt gaaagaatgc 3001 tttgtggaac ttggcttaaa tataggacaa ctgggtatag atgattctac acaagtgcct 3061 cctgaacttt ttgaaaatga acatgtacgt attgggcaaa aagttctagc agaacaagat 3121 agtgctgctg ctcaacagta catcagacaa ggaagtccca cggcactgag agctgaattg 3181 tgggctctca ttttgaatat ttccagccaa cctgaggatg tcttgtatta tgagcagctt 3241 aagaccaatg tgatacaaca tgaccttttg gtggacagtc taatctataa agatgtgaag 3301 ttgacagcaa gcaatgatga ttattatttt gtatttgaag attatttata tcaggtatta 3361 ctttgttttt cccgggatac atctgtgttg agtcactttg cattcaacag tgcctcgcca 3421 ccaaaatcat acataagagg aaaactagga ctggaagaat atgctgtctt ttacccacca 3481 aatggtgtta tcccttttca tggattttca atgtatgttg caccactttg ttttctatac 3541 catgaacctt ccaaattgta tcagatattc cgtgagatgt atgtgcgttt tttcttcaga 3601 ctccattcca tctcttctca tccttctggt attgtgtcac tctgtctgct gtttgaaact 3661 cttcttcaaa cttatcttcc ccaactcttt tatcatctac gagaaattgg ggctcaacca 3721 cttcgcatat catttaagtg gatggttcga gctttctctg gatacttagc tacagatcag 3781 ctcttgcttt tatgggatag aatcctagga tacaactctc tggaaattct tgctgtgctg 3841 gcagctgccg tgtttgcttt ccgagcagtg aacctgatgg aggtgacatc actggctgca 3901 gctgaagcag ttcttgctga cctttctact ttaaaagtta tgcctcttct tcagattttt 3961 ctgtttgcta ctgtcacctg aggatccgat atctagcggc cgcgaaggat ctgcgatcgc 4021 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 4081 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 4141 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 4201 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 4261 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 4321 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 4381 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 4441 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 4501 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagatgac 4561 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac 4621 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca 4681 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg 4741 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt 4801 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg 4861 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc 4921 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc 4981 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac 5041 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt 5101 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagtcga 5161 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 5221 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 5281 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 5341 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 5401 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 5461 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 5521 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct 5581 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 5641 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 5701 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt 5761 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 5821 gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt 5881 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 5941 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 6001 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 6061 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 6121 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 6181 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 6241 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca 6301 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 6361 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga 6421 cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 6481 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 6541 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 6601 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 6661 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 6721 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 6781 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 6841 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 6901 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 6961 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 7021 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 7081 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 7141 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 7201 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 7261 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 7321 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 7381 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 7441 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 7501 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 7561 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 7621 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 7681 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 7741 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 7801 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 7861 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 7921 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 7981 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 8041 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 8101 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 8161 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 8221 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 8281 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 8341 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 8401 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 8461 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 8521 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 8581 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 8641 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 8701 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 8761 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 8821 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 8881 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 8941 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 9001 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 9061 caagctg //