LOCUS       V046861                12436 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046861
VERSION     V046861
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6462
                     /label="TDRD9(NM_153046)"
                     /note="TDRD9(NM_153046)"
                     /gene="TDRD9"
     CDS             6469..6534
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6578..6789
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6802..7070
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7099..7108
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7129..7791
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7861..7914
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7915..8514
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8515..9103
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9177..9410
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9482..9603
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9643..9778
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9811..9827)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9835..9851
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9859..9889)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9904..9925
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10213..10801)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10972..11832)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11833..11937)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12411..12427
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc tgcggaagct caccatcgag
     2341 cagatcaacg actggttcac catcggcaag acggtgacca atgtggagct gctgggcgcg
     2401 ccgcccgcct tcccggcagg ggcggccagg gaggaggtgc agcgccagga cgtggccccc
     2461 ggcgctggtc ccgcggccca ggctccggct ctggcccaag ctccggcccg gccggccgct
     2521 gcgttcgaaa ggtcactcag ccaaaggagc tcagaagtag agtatattaa caaatacaga
     2581 cagctcgaag cacaagagct tgatgtgtgt cgcagtgtcc aaccaaccag tgggccaggt
     2641 ccaaggccat ctttggctaa attaagcagt gtgacatgca tcccagggac aacttataaa
     2701 tatcctgatt tgcctataag tcgatacaag gaagaggttg tgtctttgat agaaagtaat
     2761 tccgtggtga ttatccatgg ggccacggga agcggtaaaa gcactcagct cccgcagtat
     2821 atcttggacc actacgttca gcgctccgcc tactgcagca ttgtggtcac ccagccccgg
     2881 aagatagggg caagcagcat cgccaggtgg atcagtaaag agcgtgcctg gaccctggga
     2941 ggtgtggtgg gctaccaggt agggctagag aaaatagcaa cagaggacac caggctaatt
     3001 tatatgacaa ctggagtcct gcttcagaaa atagttagtg ccaagagttt gatggaattc
     3061 acacatatca tcattgatga agtacacgaa cgaacagaag aaatggattt cctgctattg
     3121 gtagtccgca aactcttaag aacaaattca cgttttgtga aggtggtcct gatgtcggct
     3181 accatcagct gtaaagagtt tgcagactac tttgctgttc ctgttcaaaa caagatgaat
     3241 cctgcatata tttttgaagt ggaaggcaag ccccattcag ttgaagagta ttatcttaat
     3301 gatttggagc acattcatca tagcaagctc tctcctcatc tcctggagga accggtgata
     3361 actaaggata tatatgaagt tgctgtctct ctcattcaga tgtttgatga cttggatatg
     3421 aaggagagtg ggaacaaggc ttggtcgggg gcccagtttg tgttggagcg aagcagtgtg
     3481 ttggtgtttt tgccaggtct gggtgaaata aattatatgc atgaacttct cacaagcctg
     3541 gttcataaaa ggttgcaggt ctatccactc cattcaagtg tggctttaga agaacagaat
     3601 aatgtctttt taagtccagt ccctgggtac agaaagatta ttctgtccac caatattgca
     3661 gagagttctg tcacagttcc agatgtcaaa tatgttatag atttttgttt gactagaact
     3721 ttggtctgtg atgaagatac aaattatcag agtctgcgat tgagttgggc ttctaaaacc
     3781 agctgtaatc agagaaaagg ccgtgctgga cgagtgtcta gagggtactg ttaccggctg
     3841 gtacacaagg atttctggga caactccatc cctgatcatg ttgttcctga gatgttgcgt
     3901 tgtccattag gaagcacgat cttgaaagtg aaattacttg acatgggtga gccgagagct
     3961 ctgctggcca ctgccctttc cccgcctggt ctgagtgaca ttgagcgcac catccttcta
     4021 ctaaaggagg ttggagcact tgcagtgagt gggcagagag aagatgaaaa cccccatgat
     4081 ggtgaattga ccttcttagg aagagtttta gcccaacttc ctgtaaatca gcaacttggt
     4141 aaactcatag tccttggaca tgtatttgga tgtctagatg aatgtcttat tatagcggca
     4201 gctctttctt tgaagaattt ttttgcaatg cctttccggc agcatctcga tggatatagg
     4261 aacaaagtga atttctctgg cagtagcaag agtgactgta ttgcacttgt tgaggcattt
     4321 aaaacatgga aggcttgcag acagacaggg gagctgcggt acccgaagga tgaacttaat
     4381 tggggacggt taaattacat tcaaatcaag agaattagag aggtggctga attatatgaa
     4441 gaattgaaga ctagaatctc acagttcaac atgcatgttg attctcggcg acctgtcatg
     4501 gaccaagagt atatatataa gcagcgattc atcctacagg ttgtattggc aggtgctttc
     4561 tatccaaatt actttacttt tggacagccg gatgaggaga tggcggtgag ggagctggct
     4621 ggcaaggacc ccaagacaac tgtcgtgttg aaacacattc ctccctatgg atttctttac
     4681 tataaacaac tacagtctct ctttagacag tgtggtcaag tcaaatccat tgtatttgat
     4741 ggtgcaaaag cctttgtgga attctcacga aatccaacag agagatttaa aacccttcct
     4801 gcagtatata tggcaattaa gatgtctcaa ctaaaagttt cacttgaact cagcgttcat
     4861 tctgcagagg aaattgaagg gaaggtgcaa ggcatgaacg tctcaaagct caggaacaca
     4921 agggtgaatg tggacttcca gaagcagacg gtagatccta tgcaagtctc ctttaacaca
     4981 tcagacaggt cccagacagt tacagatctc cttctaacta ttgatgtcac agaggtggtt
     5041 gaagtgggac acttttgggg atacaggatt gatgaaaaca actcagagat tctgaaaaag
     5101 cttactgctg aaatcaacca actgacgctg gtgcccttgc ccactcaccc acatccagac
     5161 ttggtctgtc tggcaccttt tgctgatttt gataaacaac gctactttag agctcaagtc
     5221 ctttatgttt ctggaaattc tgctgaggta ttctttgtag attatggcaa taagtctcat
     5281 gtagatctac atcttttgat ggagattccc tgtcaatttc ttgaacttcc tttccaggct
     5341 ttggaattta agatttgcaa aatgagacca tcagcaaagt ctcttgtttg tggcaagcac
     5401 tggagtgacg gggccagcca gtggttcgcc tctctggtga gcggctgcac cctccttgtg
     5461 aaggtcttct ctgtggtgca cagcgtcctg cacgtggatg tgtaccagta ctcaggggtc
     5521 caggatgcca tcaacataag agacgtcctc atccagcagg gctatgccga gctcacggag
     5581 gagtcctacg agtccaagca aagccatgaa gttctcaagg gcctcttttc caagtcagta
     5641 gaaaacatga cagatggctc tgtgcccttt cccatgaaag acgacgagaa atatctcatc
     5701 cggattttgt tagagagctt ttctaccaat aaactgggta ctccaaactg taaggcagaa
     5761 cttcacgggc cttttaaccc ttatgaacta aagtgccata gtttgaccag aatatccaaa
     5821 ttcaggtgtg tttggattga gaaggagagc atcaactctg tcattatcag tgacgcccct
     5881 gaagaccttc accagagaat gctggttgca gcttcccttt ccatcaatgc gactggatct
     5941 acgatgctgc tgagagaaac ctctctgatg cctcatatcc ctggcctccc ggctctcctc
     6001 agcatgttat tcgcaccggt gatagagtta aggattgatc agaatggcaa gtactatact
     6061 ggagtccttt gtggtttggg gtggaatcca gctacagggg cttccatact gcccgagcac
     6121 gacatggagc ttgcgtttga cgttcaattc agcgtggagg atgtcgtcga ggttaatatt
     6181 ctcagggctg ctattaacaa gctagtctgt gatggaccaa atggatgcaa gtgtcttggg
     6241 ccagagagag ttgcgcagct tcaagacatt gcccgtcaga agcttttagg tttgttctgt
     6301 cagtcaaaac caagggagaa gattgttccc aagtggcatg aaaagcccta cgagtggaat
     6361 caggttgatc caaagctggt catggagcag gccgaccgtg agagcagcag agggaagaac
     6421 acctttctct accagctcca caaactggtt gtgctcggca ccggatccga ctacaaagac
     6481 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     6541 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     6601 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     6661 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     6721 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     6781 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     6841 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     6901 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     6961 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     7021 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     7081 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     7141 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     7201 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     7261 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     7321 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     7381 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     7441 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     7501 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     7561 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     7621 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     7681 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     7741 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     7801 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     7861 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     7921 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     7981 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     8041 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     8101 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     8161 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     8221 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     8281 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     8341 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     8401 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     8461 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     8521 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     8581 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     8641 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     8701 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     8761 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     8821 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     8881 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     8941 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     9001 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     9061 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
     9121 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     9181 agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg
     9241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     9301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     9361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     9421 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     9481 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     9541 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     9601 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     9661 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9721 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9781 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     9841 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9901 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9961 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    10021 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    10081 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    10141 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    10201 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    10261 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    10321 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10381 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    10441 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    10501 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10561 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10621 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    10681 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    10741 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    10801 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10861 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    10921 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    10981 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    11041 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    11101 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    11161 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    11221 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    11281 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    11341 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    11401 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    11461 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    11521 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    11581 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11641 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    11701 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    11761 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    11821 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    11881 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    11941 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    12001 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    12061 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    12121 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    12181 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    12241 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
    12301 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    12361 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    12421 ggccagtgcc aagctg
//
