LOCUS       V036288                11551 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036288
VERSION     V036288
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5577
                     /label="TNK2(NM_001387707)"
                     /note="TNK2(NM_001387707)"
                     /gene="TNK2"
     CDS             5584..5649
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5693..5904
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5917..6185
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6214..6223
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6244..6906
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6976..7029
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7030..7629
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7630..8218
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8292..8525
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8597..8718
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8758..8893
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8926..8942)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8950..8966
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8974..9004)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9019..9040
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9328..9916)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10087..10947)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10948..11052)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11526..11542
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc cggcagcacg tcggttccct
     2341 ggcctggagc tctccttccc tctcctggcc agactgcggc gacgcctgta cacaaggctg
     2401 ggaggcggca gaatgcagcc agaggagggc acaggctggc tgctggagct gctgtccgag
     2461 gtgcagctgc aacagtactt cctgcggctc cgagatgacc tcaacgtcac ccgcctgtcc
     2521 cactttgagt acgtcaagaa tgaggacctg gagaagatcg gcatgggtcg gcctggccag
     2581 cggcggctgt gggaggctgt gaagaggagg aaggccttgt gcaaacgcaa gtcgtggatg
     2641 agtaaggtgt tcagtggaaa gcgactggag gctgagttcc cacctcatca ctctcagagc
     2701 accttccgga agacctcgcc cgcccctggg ggcccagcag gggaggggcc cctgcagagc
     2761 ctcacctgcc tcattgggga gaaggacctg cgcctcctgg agaagctggg tgatggttcc
     2821 tttggcgtgg tgcgcagggg cgagtgggac gcgccctcag ggaagacggt gagtgtggct
     2881 gtgaagtgcc tgaagcccga tgtcctgagc cagccagaag ccatggacga cttcatccgg
     2941 gaggtcaatg ccatgcactc gctcgaccac cgaaacctca tccgcctcta cggggtggtg
     3001 ctcacgccgc ccatgaagat ggtgacagag ctggcacctc tgggatcgtt gttggaccgg
     3061 ctacgtaagc accagggcca cttcctcctg gggactctga gccgctacgc tgtgcaggtg
     3121 gctgagggca tgggctacct ggagtccaag cgctttattc accgtgacct ggctgcccgc
     3181 aatctgctgt tggctacccg cgacctggtc aagatcgggg actttgggct gatgcgagca
     3241 ctacctcaga atgacgacca ttacgtcatg caggaacatc gcaaggtgcc cttcgcctgg
     3301 tgtgcccccg agagcctgaa gacacgcacc ttctcccatg ccagcgacac ctggatgttc
     3361 ggggtgacac tgtgggaaat gttcacctac ggccaggagc cctggatcgg cctcaacggc
     3421 agtcagatcc tgcataagat cgacaaggag ggggagcggc tgccccggcc cgaggactgt
     3481 ccccaggaca tctacaacgt catggtccag tgctgggctc acaagccaga ggacagaccc
     3541 acgtttgtgg ccctgcggga cttcctgctg gaggcccagc ccacagacat gcgggccctt
     3601 caggactttg aggaaccgga caagctgcac atccagatga atgatgtcat caccgtcatc
     3661 gagggaaggg ccgagaacta ctggtggcgt ggccagaaca cacggacgct gtgtgtgggg
     3721 cccttccctc gcaacgtggt gacctccgtg gccggcctgt cggcccagga catcagccag
     3781 cccctgcaga acagcttcat ccacacaggg catggcgaca gtgacccccg ccactgctgg
     3841 ggcttcccgg acaggattga cgaactgtat ctgggaaacc ccatggaccc ccccgacctc
     3901 ctgagcgtgg aactgagcac ctcccggccc ccccagcatc taggaggggt gaaaagggag
     3961 cctccacctc gcccacctca gcctgccttc ttcactcaga aaccaaccta tgaccctgtg
     4021 agcgaggacc aagacccctt gtccagcgac ttcaagaggc tgggcctgcg gaagccaggc
     4081 ctgccccgag ggctgtggct ggcgaagccc tcggcgcggg tgccgggcac caaggccagc
     4141 cgaggcagcg gggctgaggt cacgctcatc gacttcggtg aggagcccgt ggtcccggcc
     4201 ctacggccct gcgcgccctc cctggcgcag ctggccatgg acgcctgctc cctgctggac
     4261 gagaccccgc ctcagagccc cacgcgggca ctgccccggc ccctgcaccc cacgcctgtg
     4321 gtggactggg acgcacgccc gctgcccccc ccgcccgcct atgacgacgt ggcccaggat
     4381 gaggatgact ttgagatctg ctccatcaac agcaccctcg tgggcgcggg ggtccctgcc
     4441 gggcccagcc agggccagac caactacgcc tttgtgcctg agcaggcgcg gccgccccct
     4501 cccctggagg acaacctgtt cctcccgccc cagggtgggg gcaagccgcc cagctccgca
     4561 cagaccgcag agatcttcca ggcgctacag caggagtgca tgaggcaact gcaggctccg
     4621 gccggctccc cggccccctc tcccagcccg gggggtgacg acaagcccca ggtgcctcct
     4681 cgggtaccca tcccccctcg gcccacgcgc ccacacgtcc agctgtctcc agcccccccg
     4741 ggcgaggagg agaccagcca gtggcctgga cctgcttccc ctccccgggt gcctccgcgg
     4801 gagcccctgt cccctcaagg ctcgaggaca cccagccccc tggtaccacc tggcagctcc
     4861 ccgctgccac cccggctctc aagctcacct gggaagacca tgcccaccac ccagagcttt
     4921 gcctcagacc ccaagtacgc caccccccag gtgatccagg cccctggccc gcgggctggt
     4981 ccctgcatcc tgcccatcgt ccgggatggc aagaaggtca gcagcaccca ctattacttg
     5041 ctgcccgagc gaccatccta cctggagcgc taccagcgct tcctgcgtga ggcccagagc
     5101 cccgaggagc ctacccccct gcctgtgcct ctgctgctgc ccccacccag caccccagcc
     5161 cccgccgccc ccacggccac cgtgcggccg atgccccagg ctgccttgga ccccaaggcc
     5221 aacttctcca ccaacaacag caacccaggg gcccggccac cacccccgag ggccactgct
     5281 cggctgccac agaggggctg ccctggcgat gggccagagg cgggccggcc agcagacaag
     5341 atccagatgc tgcaggccat ggtgcatggg gtgaccacag aggagtgcca ggcggccctg
     5401 cagtgccacg gctggagcgt gcagagggct gcccagtatc tgaaggtgga gcagctcttc
     5461 gggctgggtc tgcggcccag aggggagtgc cacaaagtgc tggagatgtt cgactggaac
     5521 ctggagcagg ccggctgcca ccttctgggc tcctggggcc ctgcccacca caagcgcgga
     5581 tccgactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac
     5641 gatgacaagt agcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag
     5701 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     5761 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     5821 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     5881 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     5941 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     6001 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     6061 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     6121 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     6181 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc
     6241 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag
     6301 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc
     6361 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc
     6421 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac
     6481 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc
     6541 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc
     6601 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag
     6661 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg
     6721 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc
     6781 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg
     6841 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc
     6901 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc
     6961 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
     7021 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     7081 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     7141 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     7201 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     7261 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     7321 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     7381 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     7441 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     7501 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     7561 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     7621 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     7681 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     7741 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     7801 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     7861 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     7921 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     7981 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     8041 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     8101 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     8161 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     8221 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     8281 aaagggggga ctggaagggc taattcactc ccaacgaaga taagatctgc tttttgcttg
     8341 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     8401 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     8461 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8521 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     8581 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     8641 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8701 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     8761 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8821 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     8881 ttttttggag gcctagactt ttgcagagac caaattcgta atcatgtcat agctgtttcc
     8941 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     9001 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     9061 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     9121 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     9181 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     9241 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     9301 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     9361 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     9421 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     9481 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     9541 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9601 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     9661 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     9721 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     9781 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9841 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9901 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9961 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    10021 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    10081 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    10141 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    10201 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    10261 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    10321 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    10381 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    10441 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    10501 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    10561 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    10621 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    10681 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    10741 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    10801 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    10861 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10921 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10981 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    11041 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
    11101 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
    11161 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
    11221 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
    11281 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
    11341 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
    11401 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    11461 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
    11521 acgttgtaaa acgacggcca gtgccaagct g
//
