LOCUS       V039451                13168 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039451
VERSION     V039451
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..7194
                     /label="TOP2B(NM_001330700)"
                     /note="TOP2B(NM_001330700)"
                     /gene="TOP2B"
     CDS             7201..7266
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7310..7521
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7534..7802
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7831..7840
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7861..8523
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8593..8646
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8647..9246
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9247..9835
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9909..10142
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10214..10335
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10375..10510
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10543..10559)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10567..10583
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10591..10621)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10636..10657
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10945..11533)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11704..12564)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12565..12669)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13143..13159
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ccaagtcggg tggctgcggc
     2341 gcgggagccg gcgtgggcgg cggcaacggg gcactgacct gggtgactct ttttgatcag
     2401 aacaatgctg caaaaaaaga agagtcagaa actgccaaca aaaatgattc ttcaaagaag
     2461 ttgtctgttg agagagtgta tcagaagaag acacaacttg aacacattct tcttcgtcct
     2521 gatacatata ttgggtcagt ggagccattg acgcagttca tgtgggtgta tgatgaagat
     2581 gtaggaatga attgcaggga ggttaccttt gtgccaggtt tatacaagat ctttgatgaa
     2641 attttggtta atgctgctga caataaacag agggataaga acatgacttg tattaaagtt
     2701 tctattgatc ctgaatctaa cattataagc atttggaata atgggaaagg cattccagta
     2761 gtagaacaca aggtagagaa agtttatgtt cctgctttaa tttttggaca gcttttaaca
     2821 tccagtaact atgatgatga tgagaaaaaa gttacaggtg gtcgtaatgg ttatggtgca
     2881 aaactttgta atattttcag tacaaagttt acagtagaaa cagcttgcaa agaatacaaa
     2941 cacagtttta agcagacatg gatgaataat atgatgaaga cttctgaagc caaaattaaa
     3001 cattttgatg gtgaagatta cacatgcata acattccaac cagatctgtc caaatttaag
     3061 atggaaaaac ttgacaagga tattgtggcc ctcatgacta gaagggcata tgatttggct
     3121 ggttcgtgta gaggggtcaa ggtcatgttt aatggaaaga aattgcctgt aaatggattt
     3181 cgcagttatg tagatcttta tgtgaaagac aaattggatg aaactggggt ggccctgaaa
     3241 gttattcatg agcttgcaaa tgaaagatgg gatgtttgtc tcacattgag tgaaaaagga
     3301 ttccagcaaa tcagctttgt aaatagtatt gcaactacaa aaggtggacg gcacgtggat
     3361 tatgtggtag atcaagttgt tggtaaactg attgaagtag ttaagaaaaa gaacaaagct
     3421 ggtgtatcag tgaaaccatt tcaagtaaaa aaccatatat gggtttttat taattgcctt
     3481 attgaaaatc caacttttga ttctcagact aaggaaaaca tgactctgca gcccaaaagt
     3541 tttgggtcta aatgccagct gtcagaaaaa ttttttaaag cagcctctaa ttgtggcatt
     3601 gtagaaagta tcctgaactg ggtgaaattt aaggctcaga ctcagctgaa taagaagtgt
     3661 tcatcagtaa aatacagtaa aatcaaaggt attcccaaac tggatgatgc taatgatgct
     3721 ggtggtaaac attccctgga gtgtacactg atattaacag agggagactc tgccaaatca
     3781 ctggctgtgt ctggattagg tgtgattgga cgagacagat acggagtttt tccactcagg
     3841 ggcaaaattc ttaatgtacg ggaagcttct cataaacaga tcatggaaaa tgctgaaata
     3901 aataatatta ttaaaatagt tggtctacaa tataagaaaa gttacgatga tgcagaatct
     3961 ctgaaaacct tacgctatgg aaagattatg attatgaccg atcaggatca agatggttct
     4021 cacataaaag gcctgcttat taatttcatc catcacaatt ggccatcact tttgaagcat
     4081 ggttttcttg aagagttcat tactcctatt gtaaaggcaa gcaaaaataa gcaggaactt
     4141 tccttctaca gtattcctga atttgacgaa tggaaaaaac atatagaaaa ccagaaagcc
     4201 tggaaaataa agtactataa aggattgggt actagtacag ctaaagaagc aaaggaatat
     4261 tttgctgata tggaaaggca tcgcatcttg tttagatatg ctggtcctga agatgatgct
     4321 gccattacct tggcatttag taagaagaag attgatgaca gaaaagaatg gttaacaaat
     4381 tttatggaag accggagaca gcgtaggcta catggcttac cagagcaatt tttatatggt
     4441 actgcaacaa agcatttgac ttataatgat ttcatcaaca aggaattgat tctcttctca
     4501 aactcagaca atgaaagatc tataccatct cttgttgatg gctttaaacc tggccagcgg
     4561 aaagttttat ttacctgttt caagaggaat gataaacgtg aagtaaaagt tgcccagttg
     4621 gctggctctg ttgctgagat gtcggcttat catcatggag aacaagcatt gatgatgact
     4681 attgtgaatt tggctcagaa ctttgtggga agtaacaaca ttaacttgct tcagcctatt
     4741 ggtcagtttg gaactcggct tcatggtggc aaagatgctg caagccctcg ttatattttc
     4801 acaatgttaa gcactttagc aaggctactt tttcctgctg tggatgacaa cctccttaag
     4861 ttcctttatg atgataatca acgtgtagag cctgagtggt atattcctat aattcccatg
     4921 gttttaataa atggtgctga gggcattggt actggatggg cttgtaaact acccaactat
     4981 gatgctaggg aaattgtgaa caatgtcaga cgaatgctag atggcctgga tcctcatccc
     5041 atgcttccaa actacaaaaa ctttaaaggc acgattcaag aacttggtca aaaccagtat
     5101 gcagtcagtg gtgaaatatt tgtagtggac agaaacacag tagaaattac agagcttcca
     5161 gttagaactt ggacacaggt atataaagaa caggttttag aacctatgct aaatggaaca
     5221 gataaaacac cagcattaat ttctgattat aaagaatatc atactgacac aactgtgaaa
     5281 tttgtggtga aaatgactga agagaaacta gcacaagcag aagctgctgg actgcataaa
     5341 gtttttaaac ttcaaactac tcttacttgt aattccatgg tactttttga tcatatggga
     5401 tgtctgaaga aatatgaaac tgtgcaagac attctgaaag aattctttga tttacgatta
     5461 agttattacg gtttacgtaa ggagtggctt gtgggaatgt tgggagcaga atctacaaag
     5521 cttaacaatc aagcccgttt cattttagag aagatacaag ggaaaattac tatagagaat
     5581 aggtcaaaga aagatttgat tcaaatgtta gtccagagag gttatgaatc tgacccagtg
     5641 aaagcctgga aagaagcaca agaaaaggca gcagaagagg atgaaacaca aaaccagcat
     5701 gatgatagtt cctccgattc aggaactcct tcaggcccag attttaatta tattttaaat
     5761 atgtctctgt ggtctcttac taaagaaaaa gttgaagaac tgattaaaca gagagatgca
     5821 aaagggcgag aggtcaatga tcttaaaaga aaatctcctt cagatctttg gaaagaggat
     5881 ttagcggcat ttgttgaaga actggataaa gtggaatctc aagaacgaga agatgttctg
     5941 gctggaatgt ctggaaaagc aattaaaggt aaagttggca aacctaaggt gaagaaactc
     6001 cagttggaag agacaatgcc ctcaccttat ggcagaagaa taattcctga aattacagct
     6061 atgaaggcag atgccagcaa aaagttgctg aagaagaaga agggtgatct tgatactgca
     6121 gcagtaaaag tggaatttga tgaagaattc agtggagcac cagtagaagg tgcaggagaa
     6181 gaggcattga ctccatcagt tcctataaat aaaggtccca aacctaagag ggagaagaag
     6241 gagcctggta ccagagtgag aaaaacacct acatcatctg gtaaacctag tgcaaagaaa
     6301 gtgaagaaac ggaatccttg gtcagatgat gaatccaagt cagaaagtga tttggaagaa
     6361 acagaacctg tggttattcc aagagattct ttgcttagga gagcagcagc cgaaagacct
     6421 aaatacacat ttgatttctc agaagaagag gatgatgatg ctgatgatga tgatgatgac
     6481 aataatgatt tagaggaatt gaaagttaaa gcatctccca taacaaatga tggggaagat
     6541 gaatttgttc cttcagatgg gttagataaa gatgaatata cattttcacc aggcaaatca
     6601 aaagccactc cagaaaaatc tttgcatgac aaaaaaagtc aggattttgg aaatctcttc
     6661 tcatttcctt catattctca gaagtcagaa gatgattcag ctaaatttga cagtaatgaa
     6721 gaagattctg cttctgtttt ttcaccatca tttggtctga aacagacaga taaagttcca
     6781 agtaaaacgg tagctgctaa aaagggaaaa ccgtcttcag atacagtccc taagcccaag
     6841 agagccccaa aacagaagaa agtagtagag gctgtaaact ctgactcgga ttcagaattt
     6901 ggcattccaa agaagactac aacaccaaaa ggtaaaggcc gaggggcaaa gaaaaggaaa
     6961 gcatctggct ctgaaaatga aggcgattat aaccctggca ggaaaacatc caaaacaaca
     7021 agcaagaaac cgaagaagac atcttttgat caggattcag atgtggacat cttcccctca
     7081 gacttcccta ctgagccacc ttctctgcca cgaaccggtc gggctaggaa agaagtaaaa
     7141 tattttgcag agtctgatga agaagaagat gatgttgatt ttgcaatgtt taatggatcc
     7201 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat
     7261 gacaagtagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     7321 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     7381 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     7441 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     7501 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     7561 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     7621 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     7681 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     7741 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     7801 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg
     7861 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
     7921 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc
     7981 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac
     8041 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
     8101 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
     8161 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc
     8221 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
     8281 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc
     8341 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac
     8401 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac
     8461 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc
     8521 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc
     8581 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     8641 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     8701 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     8761 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     8821 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     8881 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     8941 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     9001 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     9061 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     9121 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     9181 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     9241 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     9301 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     9361 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     9421 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     9481 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     9541 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     9601 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     9661 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     9721 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     9781 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt
     9841 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     9901 ggggggactg gaagggctaa ttcactccca acgaagataa gatctgcttt ttgcttgtac
     9961 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    10021 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    10081 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    10141 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
    10201 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    10261 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    10321 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
    10381 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
    10441 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
    10501 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt
    10561 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    10621 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    10681 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    10741 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    10801 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    10861 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    10921 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    10981 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    11041 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    11101 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    11161 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    11221 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    11281 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    11341 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
    11401 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    11461 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    11521 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    11581 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    11641 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    11701 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    11761 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    11821 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    11881 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    11941 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    12001 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    12061 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    12121 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    12181 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    12241 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    12301 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    12361 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    12421 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    12481 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    12541 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    12601 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    12661 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    12721 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
    12781 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
    12841 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
    12901 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
    12961 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
    13021 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    13081 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    13141 ttgtaaaacg acggccagtg ccaagctg
//
