LOCUS       V033450                11762 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033450
VERSION     V033450
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5943
                     /label="TRPM4(NM_017636)"
                     /note="TRPM4(NM_017636)"
                     /gene="TRPM4"
     CDS             5980..6690
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     promoter        6732..6943
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6956..7224
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             7251..7850
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7857..8445
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8519..8752
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8824..8945
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8964..9099
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9137..9153)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9161..9177
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9185..9215)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9230..9251
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9539..10127)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10298..11158)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11159..11263)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11737..11753
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggtggtg ccggagaagg agcagagctg gatccccaag
     2341 atcttcaaga agaagacctg cacgacgttc atagttgact ccacagatcc gggagggacc
     2401 ttgtgccagt gtgggcgccc ccggaccgcc caccccgcag tggccatgga ggatgccttc
     2461 ggggcagccg tggtgaccgt gtgggacagc gatgcacaca ccacggagaa gcccaccgat
     2521 gcctacggag agctggactt cacgggggcc ggccgcaagc acagcaattt cctccggctc
     2581 tctgaccgaa cggatccagc tgcagtttat agtctggtca cacgcacatg gggcttccgt
     2641 gccccgaacc tggtggtgtc agtgctgggg ggatcggggg gccccgtcct ccagacctgg
     2701 ctgcaggacc tgctgcgtcg tgggctggtg cgggctgccc agagcacagg agcctggatt
     2761 gtcactgggg gtctgcacac gggcatcggc cggcatgttg gtgtggctgt acgggaccat
     2821 cagatggcca gcactggggg caccaaggtg gtggccatgg gtgtggcccc ctggggtgtg
     2881 gtccggaata gagacaccct catcaacccc aagggctcgt tccctgcgag gtaccggtgg
     2941 cgcggtgacc cggaggacgg ggtccagttt cccctggact acaactactc ggccttcttc
     3001 ctggtggacg acggcacaca cggctgcctg gggggcgaga accgcttccg cttgcgcctg
     3061 gagtcctaca tctcacagca gaagacgggc gtgggaggga ctggaattga catccctgtc
     3121 ctgctcctcc tgattgatgg tgatgagaag atgttgacgc gaatagagaa cgccacccag
     3181 gctcagctcc catgtctcct cgtggctggc tcagggggag ctgcggactg cctggcggag
     3241 accctggaag acactctggc cccagggagt gggggagcca ggcaaggcga agcccgagat
     3301 cgaatcaggc gtttctttcc caaaggggac cttgaggtcc tgcaggccca ggtggagagg
     3361 attatgaccc ggaaggagct cctgacagtc tattcttctg aggatgggtc tgaggaattc
     3421 gagaccatag ttttgaaggc ccttgtgaag gcctgtggga gctcggaggc ctcagcctac
     3481 ctggatgagc tgcgtttggc tgtggcttgg aaccgcgtgg acattgccca gagtgaactc
     3541 tttcgggggg acatccaatg gcggtccttc catctcgaag cttccctcat ggacgccctg
     3601 ctgaatgacc ggcctgagtt cgtgcgcttg ctcatttccc acggcctcag cctgggccac
     3661 ttcctgaccc cgatgcgcct ggcccaactc tacagcgcgg cgccctccaa ctcgctcatc
     3721 cgcaaccttt tggaccaggc gtcccacagc gcaggcacca aagccccagc cctaaaaggg
     3781 ggagctgcgg agctccggcc ccctgacgtg gggcatgtgc tgaggatgct gctggggaag
     3841 atgtgcgcgc cgaggtaccc ctccgggggc gcctgggacc ctcacccagg ccagggcttc
     3901 ggggagagca tgtatctgct ctcggacaag gccacctcgc cgctctcgct ggatgctggc
     3961 ctcgggcagg ccccctggag cgacctgctt ctttgggcac tgttgctgaa cagggcacag
     4021 atggccatgt acttctggga gatgggttcc aatgcagttt cctcagctct tggggcctgt
     4081 ttgctgctcc gggtgatggc acgcctggag cctgacgctg aggaggcagc acggaggaaa
     4141 gacctggcgt tcaagtttga ggggatgggc gttgacctct ttggcgagtg ctatcgcagc
     4201 agtgaggtga gggctgcccg cctcctcctc cgtcgctgcc cgctctgggg ggatgccact
     4261 tgcctccagc tggccatgca agctgacgcc cgtgccttct ttgcccagga tggggtacag
     4321 tctctgctga cacagaagtg gtggggagat atggccagca ctacacccat ctgggccctg
     4381 gttctcgcct tcttttgccc tccactcatc tacacccgcc tcatcacctt caggaaatca
     4441 gaagaggagc ccacacggga ggagctagag tttgacatgg atagtgtcat taatggggaa
     4501 gggcctgtcg ggacggcgga cccagccgag aagacgccgc tgggggtccc gcgccagtcg
     4561 ggccgtccgg gttgctgcgg gggccgctgc ggggggcgcc ggtgcctacg ccgctggttc
     4621 cacttctggg gcgcgccggt gaccatcttc atgggcaacg tggtcagcta cctgctgttc
     4681 ctgctgcttt tctcgcgggt gctgctcgtg gatttccagc cggcgccgcc cggctccctg
     4741 gagctgctgc tctatttctg ggctttcacg ctgctgtgcg aggaactgcg ccagggcctg
     4801 agcggaggcg ggggcagcct cgccagcggg ggccccgggc ctggccatgc ctcactgagc
     4861 cagcgcctgc gcctctacct cgccgacagc tggaaccagt gcgacctagt ggctctcacc
     4921 tgcttcctcc tgggcgtggg ctgccggctg accccgggtt tgtaccacct gggccgcact
     4981 gtcctctgca tcgacttcat ggttttcacg gtgcggctgc ttcacatctt cacggtcaac
     5041 aaacagctgg ggcccaagat cgtcatcgtg agcaagatga tgaaggacgt gttcttcttc
     5101 ctcttcttcc tcggcgtgtg gctggtagcc tatggcgtgg ccacggaggg gctcctgagg
     5161 ccacgggaca gtgacttccc aagtatcctg cgccgcgtct tctaccgtcc ctacctgcag
     5221 atcttcgggc agattcccca ggaggacatg gacgtggccc tcatggagca cagcaactgc
     5281 tcgtcggagc ccggcttctg ggcacaccct cctggggccc aggcgggcac ctgcgtctcc
     5341 cagtatgcca actggctggt ggtgctgctc ctcgtcatct tcctgctcgt ggccaacatc
     5401 ctgctggtca acttgctcat tgccatgttc agttacacat tcggcaaagt acagggcaac
     5461 agcgatctct actggaaggc gcagcgttac cgcctcatcc gggaattcca ctctcggccc
     5521 gcgctggccc cgccctttat cgtcatctcc cacttgcgcc tcctgctcag gcaattgtgc
     5581 aggcgacccc ggagccccca gccgtcctcc ccggccctcg agcatttccg ggtttacctt
     5641 tctaaggaag ccgagcggaa gctgctaacg tgggaatcgg tgcataagga gaactttctg
     5701 ctggcacgcg ctagggacaa gcgggagagc gactccgagc gtctgaagcg cacgtcccag
     5761 aaggtggact tggcactgaa acagctggga cacatccgcg agtacgaaca gcgcctgaaa
     5821 gtgctggagc gggaggtcca gcagtgtagc cgcgtcctgg ggtgggtggc cgaggccctg
     5881 agccgctctg ccttgctgcc cccaggtggg ccgccacccc ctgacctgcc tgggtccaaa
     5941 gacggatccg gtggaggcgg atcaggaggc ggtggatcta tggtgagcaa gggcgaggag
     6001 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     6061 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     6121 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     6181 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     6241 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     6301 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     6361 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     6421 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     6481 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     6541 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     6601 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     6661 accggcggca tggacgagct gtacaagtag gcggccgcga aggatctgcg atcgctccgg
     6721 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     6781 cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     6841 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     6901 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc
     6961 tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag
     7021 tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag
     7081 tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact
     7141 cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt
     7201 ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag atgaccgagt
     7261 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg
     7321 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg
     7381 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     7441 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     7501 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     7561 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     7621 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     7681 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     7741 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     7801 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gtcgacaatc
     7861 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     7921 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     7981 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     8041 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     8101 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     8161 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     8221 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     8281 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     8341 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     8401 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga
     8461 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     8521 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc
     8581 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8641 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8701 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     8761 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     8821 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8881 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8941 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc
     9001 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     9061 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc
     9121 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     9181 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     9241 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     9301 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     9361 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     9421 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     9481 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     9541 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9601 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9661 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     9721 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9781 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9841 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9901 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9961 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
    10021 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    10081 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    10141 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    10201 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    10261 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    10321 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    10381 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    10441 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    10501 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10561 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10621 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10681 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    10741 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10801 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10861 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10921 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10981 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    11041 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    11101 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    11161 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    11221 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    11281 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
    11341 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    11401 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    11461 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
    11521 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    11581 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    11641 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    11701 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
    11761 tg
//
