LOCUS       V020184                 9924 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020184
VERSION     V020184
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..3967
                     /label="Taf15(NM_027427)"
                     /note="Taf15(NM_027427)"
                     /gene="Taf15"
     CDS             3974..4039
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4083..4294
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4307..4575
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4604..4613
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4634..5296
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5366..5419
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5420..6019
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6020..6607
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6681..6914
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6986..7107
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7126..7261
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7299..7315)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7323..7339
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7347..7377)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7392..7413
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7701..8289)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8460..9320)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9321..9425)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9899..9915
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgt cggattctgg aagttacagt cagtctgggg gtgagcagca
     2341 aagttattct tcttatggaa atcaaggcag tcaaggctat ggacaaacac cacaaggtta
     2401 ttctggctat gggcaaacga ctgattcttc gtatggacaa aactatggtg gttactccgg
     2461 ttatggacaa aatcaatcag gttattcaca gtcgtatggt agttatgaga atcaaaagca
     2521 gagttcatat ggccagcaat catacaataa ccagggacaa caaaacacgg agtcatcagg
     2581 aggtcaaggt ggaagagcac cttcatatgg ccagtcagac tatggtcagc aagattctta
     2641 tgaccagcag tcaggttatg atcagcatca aggctcatat gatgagcagt ccaattatca
     2701 gcaacatgat tcctataatc aaaaccaaca gtcttaccat tcacaaaggg aaaactacag
     2761 ccaccacaca caagatgacc gtcgtgatgt gagtaggtat ggagaagata atagaggata
     2821 tggcgggtca cagggaggag gtagagggcg tgggggatat gacaaggatg gaagaggtcc
     2881 tatgacagga tcaagtggtg gtgaccgcgg tggcttcaaa aattttggtg gtcacaggga
     2941 ttatggaccc agaccagatg ctgattcaga gtctgataat tcagataaca acacaatctt
     3001 tgtgcaagga cttggggagg gtgtgtctac agatcaagtg ggagagttct ttaagcaaat
     3061 aggaattatc aagactaaca aaaagactgg aaaaccaatg ataaatcttt acacagacaa
     3121 agacacagga aagccaaaag gggaagcaac agtgtcattt gatgaccctc catcagctaa
     3181 ggcagccatt gattggtttg atggaaaaga attccatgga aacatcataa aagtgtcatt
     3241 tgccactaga aggcctgaat ttatgagagg tggtggaagt ggaggtggac gccgaggccg
     3301 tggaggatat agaggccgtg gaggttttca ggggcgaggt ggagacccca agaatgggga
     3361 ctgggtttgc cctaatccgt catgtggaaa tatgaacttt gctcgaagaa actcctgtaa
     3421 tcagtgcaat gagcctagac cagaggactc acgtccctca gggggagatt tccgggggag
     3481 aggctatggt ggagaaaggg gctacagagg tcgtgggggc agaggtggag accgaggtgg
     3541 ctatggtgga gacagaagtg ggggaggtta tggtggagac aggagtgggg gtggctatgg
     3601 tggggataga ggaggtagct atggaggaga cagaggtggt tatggtgggg accgaggagg
     3661 cagctatgga ggagaccgag gtggctacgg aggagacaga ggtggctatg gaggagacag
     3721 aggtggctat ggaggtgatc gaggaggcta tggaggtgat cgaggtggat atggaggaga
     3781 ccgcagccgg ggggcctatg gaggagaccg tggtggtagt ggtagtggca gtggaggcta
     3841 cggtggagac agaagtggag gctacggtgg agaccgaagt ggtggctacg gaggagaccg
     3901 aggtggctat ggaggcaaaa tgggaggaag aaacgactac agaaatgatc agcgcaaccg
     3961 accatacgga tccgactaca aagaccatga cggtgattat aaagatcatg acatcgacta
     4021 caaggatgac gatgacaagt gagcggccgc aaggatctgc gatcgctccg gtgcccgtca
     4081 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     4141 aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     4201 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     4261 tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc
     4321 tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct
     4381 gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct
     4441 caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct
     4501 ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct
     4561 gcgccgttac agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga
     4621 cgagagcggc ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt
     4681 ggagttcgag ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa
     4741 gatgaagagc accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg
     4801 ctacggcttc taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc
     4861 catcaacaac ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct
     4921 gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt
     4981 gggcaccggc ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc
     5041 caccgtggag cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac
     5101 cttcagcctg cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa
     5161 gagcgccatc caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt
     5221 ggaggagctg cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac
     5281 ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc
     5341 cggacccggc tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt
     5401 ggaggagaat cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     5461 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     5521 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     5581 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     5641 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     5701 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     5761 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     5821 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     5881 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     5941 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     6001 ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6061 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6121 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6181 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6241 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6301 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6361 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     6421 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6481 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6541 gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttggccgcc
     6601 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6661 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct
     6721 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6781 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6841 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6901 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     6961 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     7021 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7081 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     7141 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7201 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7261 ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga
     7321 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7381 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7441 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7501 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7561 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7621 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7681 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7741 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7801 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7861 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7921 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7981 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8041 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8101 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     8161 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8221 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8281 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8341 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8401 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8461 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8521 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8581 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8641 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8701 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8761 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8821 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8881 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8941 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9001 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9061 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9121 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9181 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9241 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9301 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9361 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9421 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     9481 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     9541 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     9601 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     9661 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     9721 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
     9781 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     9841 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     9901 aaaacgacgg ccagtgccaa gctg
//
