LOCUS       V038833                10751 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038833
VERSION     V038833
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4845
                     /label="Tas1r2(NM_031873)"
                     /note="Tas1r2(NM_031873)"
                     /gene="Tas1r2"
     promoter        4910..5121
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5134..5402
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5431..5440
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5461..6123
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6193..6246
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6247..6846
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6847..7434
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7508..7741
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7813..7934
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7953..8088
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8126..8142)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8150..8166
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8174..8204)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8219..8240
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8528..9116)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9287..10147)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10148..10252)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10726..10742
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gaccccaggc gaggacactc
     2341 catttgctgt ttctcctgct gcatgctctg cctaagccag tcatgctggt agggaactcc
     2401 gactttcacc tggctgggga ctacctcctg ggtggcctct ttaccctcca tgccaacgtg
     2461 aagagcgtct ctcacctcag ctacctgcag gtgcccaagt gcaatgagta caacatgaag
     2521 gtcttgggct acaacctcat gcaggccatg cgattcgccg tggaggaaat caacaactgt
     2581 agctctctgc tgcccggcgt gctgctcggc tacgagatgg tggatgtctg ctacctctcc
     2641 aacaatatcc agcctgggct ctacttcctg tcacagatag atgacttcct gcccatcctc
     2701 aaagactaca gccagtacag gccccaagtg gtggccgtca ttggcccaga caactctgag
     2761 tccgccatca ccgtgtccaa cattctctcc tacttcctcg tgccacaggt cacatatagc
     2821 gccatcaccg acaagctgcg agacaagcgg cgcttccctg ccatgctgcg cactgtgccc
     2881 agcgccaccc accacatcga ggccatggtg caactgatgg ttcacttcca gtggaactgg
     2941 atcgtggtgc tggtgagcga tgacgattat ggccgagaga acagccacct gctgagccag
     3001 cgtctgacca acactggcga tatctgcatt gccttccagg aggttctgcc tgtaccagaa
     3061 cccaaccagg ccgtgaggcc tgaggagcag gaccaactgg acaacatcct ggacaagctg
     3121 cggcggacct cggcgcgtgt ggtggtgata ttctcgccag agctgagcct gcacaacttc
     3181 ttccgcgagg tgctgcgctg gaacttcaca ggctttgtgt ggattgcctc tgagtcctgg
     3241 gccatcgacc ctgttctaca caacctcaca gagctgcgcc acacgggcac tttcctgggc
     3301 gtcaccatcc agagggtgtc catccctggc ttcagccagt tccgagtgcg ccacgacaag
     3361 ccagagtatc ccatgcctaa cgagaccagc ctgaggacta cctgtaacca ggactgtgac
     3421 gcctgcatga acatcaccga gtcctttaac aacgttctca tgctttcggg ggagcgtgtg
     3481 gtctacagtg tgtactcggc cgtctacgcg gtagcccaca ccctccacag actcctccac
     3541 tgcaaccagg tccgctgcac caagcaaatc gtctatccat ggcagctact cagggagatc
     3601 tggcatgtca acttcacgct cctgggcaac cagctcttct tcgacgaaca aggggacatg
     3661 ccgatgctcc tggacatcat ccagtggcaa tggggcctga gccagaaccc cttccaaagc
     3721 atcgcctcct actcccccac cgagacgagg ctgacctaca ttagcaatgt gtcctggtac
     3781 acccccaaca acacggtccc catatccatg tgttctaaga gttgccagcc tgggcaaatg
     3841 aaaaaaccca taggcctcca cccgtgctgc ttcgagtgtg tggactgtcc gccgggcacc
     3901 tacctcaacc gatcagtaga tgagtttaac tgtctgtcct gcccgggttc catgtggtct
     3961 tacaagaaca acatcgcttg cttcaagcgg cggctggcct tcctggagtg gcacgaagtg
     4021 cccactatcg tggtgaccat cctggccgcc ctgggcttca tcagtacgct ggccattctg
     4081 ctcatcttct ggagacattt ccagacgccc atggtgcgct cggcgggcgg ccccatgtgc
     4141 ttcctgatgc tggtgcccct gctgctggcg ttcgggatgg tccccgtgta tgtgggcccc
     4201 cccacggtct tctcctgttt ctgccgccag gctttcttca ccgtttgctt ctccgtctgc
     4261 ctctcctgca tcacggtgcg ctccttccag attgtgtgcg tcttcaagat ggccagacgc
     4321 ctgccaagcg cctacggttt ctggatgcgt taccacgggc cctacgtctt tgtggccttc
     4381 atcacggccg tcaaggtggc cctggtggca ggcaacatgc tggccaccac catcaacccc
     4441 attggccgga ccgaccccga tgaccccaat atcataatcc tctcctgcca ccctaactac
     4501 cgcaacgggc tactcttcaa caccagcatg gacttgctgc tgtccgtgct gggtttcagc
     4561 ttcgcgtacg tgggcaagga actgcccacc aactacaacg aagccaagtt catcaccctc
     4621 agcatgacct tctccttcac ctcctccatc tccctctgca cgttcatgtc tgtccacgat
     4681 ggcgtgctgg tcaccatcat ggatctcctg gtcactgtgc tcaactttct ggccatcggc
     4741 ttggggtact ttggccccaa gtgttacatg atccttttct acccggagcg caacacttca
     4801 gcttatttca atagcatgat tcagggctac acgatgagga agagctaggg atccctgcga
     4861 tatccagcgc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     4921 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     4981 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     5041 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     5101 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     5161 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     5221 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     5281 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     5341 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     5401 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg
     5461 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
     5521 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc
     5581 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac
     5641 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
     5701 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
     5761 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc
     5821 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
     5881 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc
     5941 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac
     6001 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac
     6061 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc
     6121 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc
     6181 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     6241 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     6301 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     6361 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     6421 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     6481 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6541 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6601 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6661 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6721 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6781 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6841 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6901 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6961 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7021 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7081 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7141 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7201 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     7261 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7321 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7381 cgtctccgcc ttcgccctca gacgagtcgg atctcccttt ggccgcctcc ccgcctggta
     7441 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7501 gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact
     7561 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7621 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7681 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7741 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     7801 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7861 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7921 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg
     7981 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     8041 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca
     8101 gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8161 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8221 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8281 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8341 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8401 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8461 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8521 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8581 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8641 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8701 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8761 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8821 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8881 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8941 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     9001 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9061 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9121 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9181 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9241 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9301 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9361 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9421 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9481 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9541 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9601 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9661 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9721 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9781 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9841 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9901 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9961 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10021 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10081 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10141 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10201 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10261 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
    10321 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
    10381 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    10441 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
    10501 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
    10561 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
    10621 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    10681 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
    10741 gtgccaagct g
//
