LOCUS       V046153                11806 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046153
VERSION     V046153
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5832
                     /label="Thbs2(NM_011581)"
                     /note="Thbs2(NM_011581)"
                     /gene="Thbs2"
     CDS             5839..5904
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5948..6159
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6172..6440
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6469..6478
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6499..7161
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7231..7284
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7285..7884
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7885..8473
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8547..8780
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8852..8973
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9013..9148
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9181..9197)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9205..9221
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9229..9259)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9274..9295
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9583..10171)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10342..11202)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11203..11307)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11781..11797
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc tctgggcact ggccctgctg
     2341 gctctgggca tagggccaag agcttctgct ggtgaccacg tcaaggacac ttcatttgac
     2401 cttttcagca tcagcaacat taaccggaag accatcggtg ccaagcagtt ccgagggcct
     2461 gaccccgggg tgcccgccta ccgttttgta cggtttgact acatcccccc agtgaacaca
     2521 gatgatctca acaggattgt caagcttgca aggagaaagg agggcttctt cctcacagcc
     2581 caactgaagc aggaccgcaa gtctcgggga acgctcctgg tgttggaagg ccccggcacc
     2641 tcccagaggc agtttgagat tgtgtccaat ggcccagggg acactttgga cctcaactac
     2701 tgggtagaag gcaatcagca taccaacttc ctggaggatg tgggcctggc tgactcccag
     2761 tggaagaatg tgactgtgca ggtggccagt gacacctata gcctgtatgt gggctgcgat
     2821 cttatcgaca gtgtcaccct ggaagaacca ttctatgagc agctagaagt agacaggagc
     2881 aggatgtacg tggccaaagg tgcatctcga gagagtcact tcaggggctt gctgcagaat
     2941 gtccatctcg tgtttgcaga ttctgtggaa gatatcttaa gcaagaaagg ctgtcaacac
     3001 agccagggag ctgaagtcaa caccatcagt gaacatacag agactctcca tctgagccct
     3061 cacatcacca cagatctcgt ggtccagggt gtggagaagg cacaggaggt gtgtacgcac
     3121 tcctgcgagg agttgagcaa catgatgaat gagctctctg gactgcacgt catggtgaac
     3181 cagctgagca agaacctgga gagagtgtct agtgataacc agttcctttt ggagctcatt
     3241 gggggccctc tgaagacaag aaacatgtca gcctgtgtgc aggagggccg aatctttgca
     3301 gaaaatgaaa cctgggttgt ggatagttgt accacatgca cctgcaagaa atttaaaaca
     3361 gtctgccatc agatcacctg ctcacctgca acttgtgcca acccatcttt tgtggaaggc
     3421 gagtgctgtc catcctgttc acactctgca gacagtgatg agggctggtc tccgtgggca
     3481 gagtggaccg agtgttctgt cacctgtggc tctgggaccc agcagagagg ccggtcttgt
     3541 gatgtcacca gcaacacctg cctgggcccc tccattcaga caaggacatg cagcctgggc
     3601 aaatgtgata cgagaatccg tcagaatgga ggctggagtc actggtcacc ctggtcttca
     3661 tgctccgtga cttgtggagt tggcaatgtc acccgcatac gtctctgcaa ctcaccagtg
     3721 ccccagatgg gtggcaagaa ctgcaagggc agcggccggg aaaccaaacc ctgtcagcgt
     3781 gatccgtgcc caattgatgg ccgctggagc ccctggtccc cttggtcagc ctgcacagtt
     3841 acctgtgctg gagggatccg tgagcgctca cgtgtttgca acagccctga gccccagtat
     3901 ggagggaagg actgtgtcgg ggatgtgaca gaacaccaaa tgtgcaacaa gagaagctgc
     3961 cctattgatg ggtgcttatc caacccgtgt tttcctggag ccaagtgcaa cagcttccct
     4021 gatgggtcct ggtcctgtgg ctcctgccca gtgggctttc tgggcaatgg tacccactgt
     4081 gaggacctgg atgagtgtgc tgtggtcaca gatatttgct tctcaactaa caaagctccc
     4141 cgctgtgtca acaccaaccc gggcttccac tgcctgcctt gtccaccacg ctacaagggg
     4201 aaccaaccct tcggtgttgg cctggaggat gctaggacag aaaaacaagt gtgtgagcca
     4261 gagaatccat gtaaggacaa gactcacagc tgccacaaga atgcagagtg catctacctg
     4321 ggccacttta gtgaccccat gtacaagtgt gagtgccaga ttggctacgc aggtgatggg
     4381 ctcatctgcg gggaggactc agacctggat ggctggccca acaacaacct ggtgtgtgct
     4441 actaatgcca cctaccactg catcaaggac aactgcccca aactgccaaa ttccgggcag
     4501 gaggattttg ataaggatgg aatcggagat gcttgtgacg aggacgatga caatgacggt
     4561 gtgagcgatg agaaggacaa ttgccagctt ctcttcaatc cccgtcaatt agactatgac
     4621 aaggatgagg ttggagaccg ctgtgacaac tgcccctatg tgcacaaccc agcacagatc
     4681 gacacagaca acaatggcga gggggatgcc tgctctgtgg acattgacgg agacgatgtt
     4741 ttcaatgagc gagacaattg tccatatgtc tacaacactg accagagaga tacggatggt
     4801 gatggcgtgg gtgaccactg tgacaattgt cctctgatgc acaacccaga tcagatcgat
     4861 caggacaatg atctcgttgg agaccagtgt gacaacaatg aggacataga tgatgacggc
     4921 caccagaaca accaagacaa ctgcccatac atctccaact ccaaccaggc tgaccatgac
     4981 aacgacggca agggcgatgc ctgcgactct gatgatgaca atgatggtgt tccagatgac
     5041 agggacaact gtcggcttgt gttcaaccca gaccaggaag actcggacgg tgacggccga
     5101 ggtgacattt gtaaagatga ctttgacaat gataatgtcc cagatattga tgatgtgtgc
     5161 cctgagaaca atgccatcac tgagacagac ttcagaaact tccagatggt ccctctggat
     5221 cccaagggga ccacacaaat tgatcccaac tgggtaattc gtcaccaagg caaagagctg
     5281 gtgcagacag caaactcaga ccctggcatc gctgtaggtt tcgacgagtt tgggtctgtg
     5341 gacttcagtg gcactttcta tgtcaacact gaccgggatg atgactacgc tggctttgtc
     5401 tttggctatc agtcaagcag ccgcttctat gtggtgatgt ggaagcaggt gacccagacc
     5461 tactgggaag acaagcccag tcgggcttac ggctactctg gtgtgtcact caaagtggta
     5521 aactccacga ctggtactgg cgagcacctg aggaatgccc tgtggcacac gggaaacaca
     5581 gaaggccagg tccggactct atggcatgac cccaaaaaca ttggctggaa agactacact
     5641 gcctacaggt ggcacctgat tcacaggcct aagacaggct acatgagagt cttagtgcat
     5701 gaaggaaagc aagtcatggc tgactcagga ccaatttatg accaaaccta cgctggtgga
     5761 cggctgggcc tgtttgtctt ctcccaagag atggtctatt tctcggacct caagtatgag
     5821 tgcagagatg ccggatccga ctacaaagac catgacggtg attataaaga tcatgacatc
     5881 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc
     5941 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     6001 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     6061 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     6121 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
     6181 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     6241 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     6301 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     6361 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     6421 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag
     6481 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac
     6541 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc
     6601 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg
     6661 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg
     6721 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc
     6781 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag
     6841 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc
     6901 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc
     6961 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac
     7021 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc
     7081 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc
     7141 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc
     7201 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt
     7261 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc
     7321 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     7381 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     7441 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     7501 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     7561 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     7621 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     7681 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     7741 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     7801 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     7861 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg
     7921 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7981 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     8041 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     8101 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     8161 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     8221 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     8281 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     8341 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     8401 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     8461 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     8521 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga
     8581 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     8641 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8701 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8761 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     8821 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     8881 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8941 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     9001 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     9061 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     9121 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat
     9181 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9241 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9301 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9361 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9421 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9481 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9541 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9601 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9661 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9721 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9781 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9841 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9901 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9961 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10021 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10081 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10141 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10201 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10261 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10321 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10381 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10441 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10501 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10561 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10621 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10681 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10741 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10801 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10861 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10921 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10981 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11041 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11101 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11161 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11221 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11281 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    11341 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    11401 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    11461 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    11521 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    11581 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    11641 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    11701 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    11761 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
