LOCUS       V029092                11389 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029092
VERSION     V029092
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5415
                     /label="Topors(NM_134097)"
                     /note="Topors(NM_134097)"
                     /gene="Topors"
     CDS             5422..5487
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5531..5742
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5755..6023
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6052..6061
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6082..6744
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6814..6867
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6868..7467
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7468..8056
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8130..8363
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8435..8556
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8596..8731
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8764..8780)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8788..8804
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8812..8842)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8857..8878
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9166..9754)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9925..10785)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10786..10890)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11364..11380
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ggtcgcagcc gccgccaccg
     2341 gggtctccgc tgtctcgcga ggagggtgaa gcgccccctc tggtgcctgc cgaggagggg
     2401 aggcgccgaa gccgccgggt acgccttcgc ggatcctgcc gccaccggcc gagcttactg
     2461 agccggcggg agctggcttc caacggccca gcggttcctg ccaccgcgtc ctcggagata
     2521 atggcctcag ctgctaagga atttaaaatg gacaactttt cacctaaagc tggcactagc
     2581 aaattgcagc agacagtacc agctgatgcg tctcctgatt ctaagtgtcc tatatgcttg
     2641 gatagatttg ataatgtgtc ttacttagat cgttgcctac ataagttctg ttttcgctgt
     2701 gtacaagagt ggtccaaaaa caaagccgaa tgcccactgt gtaaacagcc ctttgattct
     2761 attttccatt cagtgagggc tgaagatgac ttcaaggagt atgtcctgag gccttcctat
     2821 aatggttcct ttactaaccc tgaggttcga aggtttcgct accggacaac tatgacgaga
     2881 gaacggagcg cctctcttta ttcacccagt agtactgtaa gtaggagaac aaccacgcca
     2941 ccagatagtg gagtgctgtt tgaagggtta ggcatttcga caagacctag agatgttgac
     3001 attcctcaat ttatgagaca aatggcatta agggggccaa ctactacaga tgagagatct
     3061 ttgcggaaaa ttcaagaaca agatattatt aatttcaggc gaactcttta tcgtgctggt
     3121 gttcgagtta gaagtattga agatggtggc cgctacaggg atatttcagc tgagttcttc
     3181 cgtagaaatc cagcttgcct tcacagatta gttccttggt taaagcgtga acttactgtg
     3241 ctttttggag cccatggttc gctagtcaat atcgtccagc acatcatcat gagtaatgtt
     3301 actcgctatg atttggagag tcaggcattt gtgtctgact taagaccatt tttgcttaat
     3361 cgaactgagc attttataca tgaatttatc agttttgcta gatctccctt caatatggca
     3421 gcttttgacc agcatgctaa ttatgattgc cctccttcat ctgaagaagg cagccgttca
     3481 gattcttcag tcattacaat atcaccagat gaggctgaaa ctcaagagct agatatgaat
     3541 gcatctactg ttaggcaggc accgtgggat gatgaaactc ctggaccatc ttattcaagt
     3601 tcagagcaag tgcatgttgg cgtgtcttcc cttttaaatt cttcagacag ttcagatgaa
     3661 gagcttgtct ctggaggaac cacatctcag atacaaggag tacagaccaa tgacgatgtc
     3721 aataatgaca gtgattcatc gtcagataat tgtgtcattg ttgggtttgt taagccattg
     3781 gctgaaagga ccccggaact tgttgaactg tcttctgatt ctgaggagtt gggcccttat
     3841 gagaaagtgg agacagtgaa gactcaggag caagagcagt cgtacagttc aggtgacagt
     3901 gatgttagcc gggcgtcatc tccacgctct gtccttggaa aagatgaaca aatgagtaaa
     3961 agccattgtg attctgacac aagaataagc tcaaagaaag aagagaaacg atctacatca
     4021 ttgccagctc ccagggacag ctcatctaca agaggtgaca gggtatgctc cccatataac
     4081 catcgacaca ggaagggagg aaggtcgagg agttcagatt cccggtccca gagtagaagt
     4141 ggccatgacc cgaggaatca caggaagcac gggaagaaaa gactgaggaa caagaggtcc
     4201 agaagcaggg agagtagtag caggccgaga gcaagaaagg acaaaaagag atccagaact
     4261 agagatagca gctggtcccg gaggagccag acactttctc taagtagtgg aagcaccagt
     4321 agatcgaggt ctcgtagcag tgatcatggc aaaagaagat cacgaagcag aaatcgagat
     4381 cgctattatt taagaaataa ttatggaagc aaatataagt gggaatacac ttactacagc
     4441 agaaacaagg acagagatgg ctatgaatca tcatacagga ggaggactct ttcaagagcc
     4501 cattactcta gacagtcttc aagtccagaa tttagaattc agtctttttc tgaaagaaca
     4561 aatgcgagga agaagaatca cagtgaaagg aaatactact actatgaaag gcgccggtcc
     4621 aggagcgtgt ccagtaacag gtcgaggact acatccgccg ggcccgaccg ggtgagaaat
     4681 gagaagcccg gagggaaacg caagtacaaa acccggcatt tggaaggtac cagtgaagag
     4741 gctcagccgg ctcgggagtt cacttccaaa gggaaggaca gtcattacca aaaatcaaaa
     4801 ttggatggca gttataaaaa tgagagtgac agcttttcag atagcagatc atcagacaga
     4861 gagacaaaac acaagaggag gaggaggagg acccggagcc taagtgtaga gatagtttat
     4921 gaaggaaagg ctacggacac aagcaaacac cataagaaga aaaagaagaa acataagaag
     4981 aagcataaga aacaccatgg agataatact tcacggtcgc cggtggttat aaccatcgac
     5041 agtgacagcg atggagagtc tgaagtaaag gcgggtattg aatgtagcaa tggcagtctt
     5101 ccacagccta tacaagatgg agcatttgaa actaaagatg ttgtcacaat cgaagatgag
     5161 cttggtgtcc tggacaagga ctgtgacgtt acagcacttg ctgatgactt gagcaccagc
     5221 caaactgtgg agaattgtga ctcgccggca gtcccagttg aacaaactct tgatgtaaga
     5281 gaagaaagca cctttgcctc tgatttggag agccagtcga gtaatgtgtc tattcaggct
     5341 gaaccatcga gaccagtgcc atctccacgg acctcactgt catcagtgtc tcctggtaga
     5401 gactgtgatg tgtctggatc cgactacaaa gaccatgacg gtgattataa agatcatgac
     5461 atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga tcgctccggt
     5521 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     5581 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     5641 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     5701 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     5761 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     5821 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     5881 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     5941 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     6001 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg
     6061 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg
     6121 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg
     6181 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac
     6241 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc
     6301 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc
     6361 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc
     6421 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc
     6481 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc
     6541 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg
     6601 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc
     6661 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc
     6721 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac
     6781 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc
     6841 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc
     6901 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     6961 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     7021 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     7081 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     7141 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     7201 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     7261 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     7321 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     7381 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     7441 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga
     7501 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7561 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7621 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7681 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7741 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7801 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7861 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7921 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7981 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8041 tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct
     8101 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata
     8161 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     8221 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8281 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8341 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     8401 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     8461 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8521 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     8581 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8641 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     8701 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat
     8761 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     8821 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8881 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8941 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     9001 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     9061 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     9121 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     9181 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9241 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9301 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9361 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9421 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9481 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9541 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9601 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9661 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9721 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9781 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9841 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9901 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9961 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    10021 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    10081 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    10141 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    10201 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    10261 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    10321 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    10381 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    10441 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    10501 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10561 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10621 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10681 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10741 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10801 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10861 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10921 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    10981 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
    11041 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    11101 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
    11161 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    11221 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    11281 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
    11341 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
