LOCUS       V022960                11368 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022960
VERSION     V022960
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..5446
                     /label="ULK1(NM_003565)"
                     /note="ULK1(NM_003565)"
                     /gene="ULK1"
     CDS             5453..5482
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     promoter        5527..5738
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5751..6019
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6048..6057
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6078..6740
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6810..6863
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6864..7463
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7464..8051
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8125..8358
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8430..8551
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8570..8705
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8743..8759)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8767..8783
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8791..8821)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8836..8857
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9145..9733)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9904..10764)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10765..10869)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11343..11359
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg agcccggccg cggcggcaca gagaccgtgg gcaagttcga
     2341 gttctcccgc aaggacctga tcggccacgg cgccttcgcg gtggtcttca agggccgcca
     2401 ccgcgagaag cacgatttgg aggtcgccgt caagtgcatt aacaagaaga acctcgccaa
     2461 gtctcagacg ctgctgggga aggaaatcaa aatcctgaag gaactgaaac atgaaaacat
     2521 cgtggccctg tacgacttcc aggaaatggc taattctgtc tacctggtta tggagtactg
     2581 caacggtggg gacctggccg actacctgca cgccatgcgc acgctgagcg aggacaccat
     2641 caggctcttc ctgcagcaga tcgcgggcgc catgcggctt ctgcacagca aaggcatcat
     2701 ccaccgcgac ctgaaaccgc agaacatcct gctgtccaac cccgccggcc gccgcgccaa
     2761 ccccaacagc atccgcgtca agatcgctga cttcggcttc gcgcggtacc tccagagcaa
     2821 catgatggcg gccacactct gcggctcccc catgtacatg gcccccgagg tcatcatgtc
     2881 ccagcactac gacgggaagg cggacctgtg gagcatcggc accatcgtct accagtgcct
     2941 gacggggaag gcgcccttcc aggccagcag cccccaggac ctgcgcctgt tctacgagaa
     3001 gaacaagacg ttggtcccca ccatcccccg ggagacctcg gccccgctgc ggcagctgct
     3061 cctggcccta ctgcaacgca accacaagga ccgcatggac ttcgatgagt tttttcatca
     3121 ccctttcctc gatgccagcc cctcggtcag gaaatcccca cccgtgcctg tgccctcgta
     3181 cccaagctcg gggtccggca gcagctccag cagcagctcc acctcccacc tggcctcccc
     3241 gccgtccctg ggcgagatgc agcagctgca gaagaccctg gcctccccgg ctgacaccgc
     3301 tggcttcctg cacagctccc gggactctgg tggcagcaag gactcttcct gtgacacaga
     3361 cgacttcgtc atggtccccg cgcagtttcc aggtgacctg gtggctgagg cgcccagtgc
     3421 caaacccccg ccagacagcc tgatgtgcag tgggagctca ctggtggcct ctgcgggctt
     3481 ggagagccac ggccggaccc catctccatc cccaccctgc agcagctccc ccagtccctc
     3541 aggccgggct ggcccgttct ccagcagcag gtgcggcgcc tctgtcccca tcccagtccc
     3601 cacgcaggtg cagaactacc agcgcattga gcgaaacctg cagtcaccca cccagttcca
     3661 aacacctcgg tcctctgcca tccgcaggtc aggcagcacc agccccctgg gctttgcaag
     3721 ggccagcccc tcgccccctg cccacgctga gcatggaggc gtcctggcca ggaagatgtc
     3781 tctgggtgga ggccggccct acacgccatc tcctcaagtt ggaaccatcc ctgagcggcc
     3841 aggctggagc gggacgccct ccccacaggg agctgagatg cggggtggca ggtcccctcg
     3901 tccaggctcc tctgcacccg agcactctcc ccgcacttcc gggctgggct gccgcctgca
     3961 cagcgccccc aacctgtctg acttgcacgt cgtccgcccc aagctgccca aaccccccac
     4021 ggaccccctg ggagctgtgt tcagcccacc acaggccagc cctccccagc cgtcccacgg
     4081 cctgcagtcc tgccggaacc tgcggggctc acccaagctg cccgacttcc tgcagcgaaa
     4141 ccccctgccc cccatcctgg gctcccccac caaggctgtg ccctcctttg acttcccgaa
     4201 gacccccagc tcccagaacc tgctggccct cctagcccgg cagggcgtgg tgatgacgcc
     4261 ccctcgaaac cggacgctgc ccgacctctc ggaggtggga cccttccatg gtcagccgtt
     4321 gggccctggc ctgcggccag gcgaggaccc caagggcccc tttggccggt ctttcagcac
     4381 cagccgcctc actgacctgc tccttaaggc ggcgtttggg acacaagccc cggacccggg
     4441 cagcacggag agcctgcagg agaagcccat ggagatcgca ccctcagctg gctttggagg
     4501 gagcctgcac ccaggagccc gtgctggggg caccagcagc ccttccccgg tggtcttcac
     4561 cgtgggctct cccccgagcg ggagcacgcc cccccagggc ccccgcacca ggatgttctc
     4621 agcgggcccc actggctctg ccagctcttc tgcccgccac ctggtgcctg ggccctgcag
     4681 cgaggcccca gcccctgagc tccctgctcc aggacacggc tgcagctttg ccgaccccat
     4741 tactgcgaac ctggaggggg ctgtgacctt cgaggccccc gacctccctg aggagaccct
     4801 catggagcaa gagcacacgg agatcctgcg tggcctgcgc ttcacgctgc tgttcgtgca
     4861 gcacgtcctg gagatcgcag ccctgaaggg cagcgccagt gaggcggcgg ggggccctga
     4921 gtaccagctg caggagagtg tggtggccga ccagatcagc ctgctgagcc gagaatgggg
     4981 cttcgcggaa cagctggtgc tgtacctgaa ggtggccgag ctactgtcct ccggcctgca
     5041 aagtgccatc gaccagatcc gggccggcaa gctctgcctg tcgtccactg tgaagcaggt
     5101 ggtgcgcagg ctgaatgagc tgtacaaggc cagcgtggtg tcctgccagg gcctgagcct
     5161 gcggctgcag cgcttcttcc tggacaagca gcggctcctg gaccgcattc acagcatcac
     5221 tgccgagagg ctcatcttca gccacgctgt gcagatggtg cagtcggctg ccctggacga
     5281 gatgttccag caccgtgagg gctgcgtccc acgctaccac aaggccctgc tgctcctgga
     5341 ggggctgcag cacatgctct cggaccaggc cgacatcgag aacgtcacca agtgcaagct
     5401 gtgcattgag cggagactct cggcgctgct gactggcatc tgtgccggat ccgaacaaaa
     5461 actcatctca gaagaggatc tgtgagcggc cgcgaaggat ctgcgatcgc tccggtgccc
     5521 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     5581 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     5641 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     5701 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
     5761 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
     5821 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
     5881 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
     5941 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
     6001 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggaga
     6061 gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg
     6121 gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca
     6181 acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga
     6241 tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc
     6301 acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg
     6361 tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg
     6421 tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca
     6481 acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc
     6541 gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact
     6601 tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc
     6661 gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca
     6721 agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca
     6781 ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg
     6841 acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     6901 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     6961 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     7021 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     7081 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     7141 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     7201 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     7261 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     7321 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     7381 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     7441 tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga
     7501 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     7561 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     7621 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     7681 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     7741 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7801 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     7861 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7921 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7981 ctctgcggcc tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggc
     8041 cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     8101 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc
     8161 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     8221 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     8281 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     8341 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     8401 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     8461 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8521 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     8581 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     8641 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     8701 aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt
     8761 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8821 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8881 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8941 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     9001 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     9061 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     9121 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9181 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     9241 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9301 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     9361 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9421 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9481 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9541 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     9601 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9661 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9721 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9781 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9841 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9901 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9961 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    10021 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    10081 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    10141 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    10201 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    10261 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    10321 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    10381 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    10441 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10501 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    10561 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10621 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10681 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10741 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10801 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10861 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    10921 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
    10981 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
    11041 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
    11101 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
    11161 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
    11221 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    11281 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    11341 ttgtaaaacg acggccagtg ccaagctg
//
