LOCUS       V038617                11227 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038617
VERSION     V038617
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5253
                     /label="USP37(NM_020935)"
                     /note="USP37(NM_020935)"
                     /gene="USP37"
     CDS             5260..5325
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5369..5580
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5593..5861
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5890..5899
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5920..6582
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6652..6705
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6706..7305
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7306..7894
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7968..8201
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8273..8394
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8434..8569
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8602..8618)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8626..8642
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8650..8680)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8695..8716
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9004..9592)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9763..10623)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10624..10728)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11202..11218
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt ctcctctgaa gatacatggt
     2341 cctatcagaa ttcgaagtat gcagactggg attacaaagt ggaaagaagg atcctttgaa
     2401 attgtagaaa aagagaataa agtcagccta gtagttcact acaatactgg aggaattcca
     2461 aggatatttc agctaagtca taacattaaa aatgtggtgc ttcgacccag tggagcgaaa
     2521 caaagccgcc taatgttaac tctgcaagat aacagcttct tgtctattga caaagtacca
     2581 agtaaggatg cagaggaaat gaggttgttt ctagatgcag tccatcaaaa cagacttcct
     2641 gcagccatga aaccgtctca ggggtctggt agttttggag ccattctggg cagcaggacc
     2701 tcacagaagg aaaccagcag gcagctttct tactcagaca atcaggcttc tgcaaaaaga
     2761 ggaagtttgg aaactaaaga tgatattcca tttcgaaaag ttcttggtaa tccgggtaga
     2821 ggatcgatta agactgtagc aggaagtgga atagctcgga cgattccttc tttgacatct
     2881 acttcaacac ctcttagatc agggttgcta gaaaatcgta ctgaaaagag gaaaagaatg
     2941 atatcaactg gctcagaatt gaatgaagat taccctaagg aaaatgattc atcatcgaac
     3001 aacaaggcca tgacagatcc ctccagaaag tatttaacca gcagtagaga aaagcagctg
     3061 agtttgaaac agtcagaaga gaataggaca tcagggcttt tacctttaca gtcatcatcc
     3121 ttttatggta gcagagctgg atccaaggaa cactcttctg gtggcactaa cttagacagg
     3181 actaatgttt caagccagac tccctctgcc aaaagaagtt tgggatttct tcctcagcca
     3241 gttcctcttt ctgttaaaaa actgaggtgt aaccaggatt acactggctg gaataaacca
     3301 agagtgcccc tttcctctca ccaacagcag caactgcagg gcttctccaa tttgggaaat
     3361 acctgctata tgaatgctat tctacaatct ctattttcac tccagtcatt tgcaaatgac
     3421 ttgcttaaac aaggtatccc atggaagaaa attccactca atgcacttat cagacgcttt
     3481 gcacacttgc ttgttaaaaa agatatctgt aattcagaga ccaaaaagga tttactcaag
     3541 aaggttaaaa atgccatttc agctacagca gagagattct ctggttatat gcagaatgat
     3601 gctcatgaat ttttaagtca gtgtttggac cagctgaaag aagatatgga aaaattaaat
     3661 aaaacttgga agactgaacc tgtttctgga gaagaaaatt caccagatat ttcagctacc
     3721 agagcataca cttgccctgt tattactaat ttggagtttg aggttcagca ctccatcatt
     3781 tgtaaagcat gtggagagat tatccccaaa agagaacagt ttaatgacct ctctattgac
     3841 cttcctcgta ggaaaaaacc actccctcct cgttcaattc aagattctct tgatcttttc
     3901 tttagggccg aagaactgga gtattcttgt gagaagtgtg gtgggaagtg tgctcttgtc
     3961 aggcacaaat ttaacaggct tcctagggtc ctcattctcc atttgaaacg atatagcttc
     4021 aatgtggctc tctcgcttaa caataagatt gggcagcaag tcatcattcc aagatacctg
     4081 accctgtcat ctcattgcac tgaaaataca aaaccacctt ttacccttgg ttggagtgca
     4141 catatggcaa tttctagacc attgaaagcc tctcaaatgg tgaattcctg catcaccagc
     4201 ccttctacac cttcaaagaa attcaccttc aaatccaaga gctccttggc tttatgcctt
     4261 gattcagaca gtgaggatga gctaaaacgt tctgtggccc tcagccagag actttgtgaa
     4321 atgttaggca acgaacagca gcaggaagac ctggaaaaag attcaaaatt atgcccaata
     4381 gagcctgaca agtctgaatt ggaaaactca ggatttgaca gaatgagcga agaagagctt
     4441 ctagcagctg tcttggagat aagtaagaga gatgcttcac catctctgag tcatgaagat
     4501 gatgataagc caactagcag cccagatacc ggatttgcag aagatgatat tcaagaaatg
     4561 ccagaaaatc cagacactat ggaaactgag aagcccaaaa caatcacaga gctggatcct
     4621 gccagtttta ctgagataac taaagactgt gatgagaata aagaaaacaa aactccagaa
     4681 ggatctcagg gagaagttga ttggctccag cagtatgata tggagcgtga aagggaagag
     4741 caagagcttc agcaggcact ggctcagagc cttcaagagc aagaggcttg ggaacagaaa
     4801 gaagatgatg acctcaaaag agctaccgag ttaagtcttc aagagtttaa caactccttt
     4861 gtggatgcat tgggttctga tgaggactct ggaaatgagg atgtttttga tatggagtac
     4921 acagaagctg aagctgagga actgaaaaga aatgctgaga caggaaatct gcctcattcg
     4981 taccggctca tcagtgttgt cagtcacatt ggtagcactt cttcttcagg tcattacatt
     5041 agtgatgtat atgacattaa gaagcaagcg tggtttactt acaatgacct ggaggtatca
     5101 aaaatccaag aggctgccgt gcagagtgat cgagatcgga gtggctacat cttcttttat
     5161 atgcacaagg agatctttga tgagctgctg gaaacagaaa agaactctca gtcacttagc
     5221 acggaagtgg ggaagactac ccgtcaggcc ttgggatccg actacaaaga ccatgacggt
     5281 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     5341 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     5401 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     5461 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     5521 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     5581 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     5641 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     5701 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     5761 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     5821 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     5881 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     5941 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     6001 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     6061 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     6121 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     6181 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     6241 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     6301 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     6361 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     6421 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     6481 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     6541 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     6601 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     6661 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     6721 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6781 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6841 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6901 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6961 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     7021 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     7081 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     7141 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     7201 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7261 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     7321 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7381 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7441 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7501 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7561 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7621 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7681 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     7741 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7801 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7861 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
     7921 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     7981 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca
     8041 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8101 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8161 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     8221 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     8281 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8341 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8401 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8461 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8521 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     8581 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8641 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8701 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8761 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8821 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8881 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8941 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9001 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9061 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9121 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9181 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9241 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9301 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9361 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9421 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     9481 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9541 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9601 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9661 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9721 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9781 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9841 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9901 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9961 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10021 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10081 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10141 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10201 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10261 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10321 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10381 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10441 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10501 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10561 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10621 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10681 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10741 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    10801 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    10861 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    10921 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
    10981 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    11041 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
    11101 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    11161 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    11221 caagctg
//
