LOCUS       V025038                12417 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025038
VERSION     V025038
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..6274
                     /label="USP42(NM_001365764)"
                     /note="USP42(NM_001365764)"
                     /gene="USP42"
     CDS             6281..6325
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic
                     ribonuclease A"
                     /transl_table=1
                     /translation="KETAAAKFERQHMDS"
                     /label="S-Tag"
     CDS             6359..6382
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             6386..6409
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             6419..6532
                     /codon_start=1
                     /note="selected from a peptide library; binds streptavidin
                     with nanomolar affinity (Keefe et al., 2001)"
                     /product="streptavidin-binding peptide"
                     /transl_table=1
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
                     /label="SBP"
     promoter        6576..6787
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6800..7068
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7097..7106
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7127..7789
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7859..7912
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7913..8512
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8513..9100
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9174..9407
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9479..9600
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9619..9754
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9792..9808)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9816..9832
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9840..9870)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9885..9906
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10194..10782)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10953..11813)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11814..11918)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12392..12408
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgaccat agttgacaaa gcttctgaat cttcagaccc
     2341 atcagcctat cagaatcagc ctggcagctc cgaggcagtc tcacctggag acatggatgc
     2401 aggttctgcc agctggggtg ctgtgtcttc attgaatgat gtgtcaaatc acacactttc
     2461 tttaggacca gtacctggtg ctgtagttta ttcgagttca tctgtacctg ataaatcaaa
     2521 accatcacca caaaaggatc aagccctagg tgatggcatc gctcctccac agaaagttct
     2581 tttcccatct gagaagattt gtcttaagtg gcaacaaact catagagttg gagctgggct
     2641 ccagaatttg ggcaatacct gttttgccaa tgcagcactg cagtgtttaa cctacacacc
     2701 acctcttgcc aattacatgc tatcacatga acactccaaa acatgtcatg cagaaggctt
     2761 ttgtatgatg tgtacaatgc aagcacatat tacccaggca ctcagtaatc ctggggacgt
     2821 tattaaacca atgtttgtca tcaatgagat gcggcgtata gctaggcact tccgttttgg
     2881 aaaccaagaa gatgcccatg aattccttca atacactgtt gatgctatgc agaaagcatg
     2941 cttgaatggc agcaataaat tagacagaca cacccaggcc accactcttg tttgtcagat
     3001 atttggagga tacctaagat ctagagtcaa atgtttaaat tgcaagggcg tttcagatac
     3061 ttttgatcca tatcttgata taacattgga gataaaggct gctcagagtg tcaacaaggc
     3121 attggagcag tttgtgaagc cggaacagct tgatggagaa aactcgtaca agtgcagcaa
     3181 gtgtaaaaag atggttccag cttcaaagag gttcactatc catagatcct ctaatgttct
     3241 tacactttct ctgaaacgtt ttgcaaattt taccggtgga aaaattgcta aggatgtgaa
     3301 ataccctgag tatcttgata ttcggccata tatgtctcaa cccaacggag agccaattgt
     3361 ctacgtcttg tatgcagtgc tggtccacac tggttttaat tgccatgctg gccattactt
     3421 ctgctacata aaagctagca atggcctctg gtatcaaatg aatgactcca ttgtatctac
     3481 cagtgatatt agatcggtac tcagccaaca agcctatgtg ctcttttata tcaggtccca
     3541 tgatgtgaaa aatggaggtg aacttactca tcccacccat agccccggcc agtcctctcc
     3601 ccgccccgtc atcagtcagc gggttgtcac caacaaacag gctgcgccag gctttatcgg
     3661 accacagctt ccctctcaca tgataaagaa tccacctcac ttaaatggga ctggaccatt
     3721 gaaagacacg ccaagcagtt ccatgtcgag tcctaacggg aattccagtg tcaacagggc
     3781 tagtcctgtt aatgcttcag cttctgtcca aaactggtca gttaataggt cctcagtgat
     3841 cccagaacat cctaagaaac aaaaaattac aatcagtatt cacaacaagt tgcctgttcg
     3901 ccagtgtcag tctcaaccta accttcatag taattctttg gagaacccta ccaagcccgt
     3961 tccctcttct accattacca attctgcagt acagtctacc tcgaacgcat ctacgatgtc
     4021 agtttctagt aaagtaacaa aaccgatccc ccgcagtgaa tcctgctccc agcccgtgat
     4081 gaatggcaaa tccaagctga actccagcgt gctggtgccc tatggcgccg agtcctctga
     4141 ggactctgac gaggagtcaa aggggctggg caaggagaat gggattggta cgattgtgag
     4201 ctcccactct cccggccaag atgccgaaga tgaggaggcc actccgcacg agcttcaaga
     4261 acccatgacc ctaaacggtg ctaatagtgc agacagcgac agtgacccga aagaaaacgg
     4321 cctagcgcct gatggtgcca gctgccaagg ccagcctgcc ctgcactcag aaaatccctt
     4381 tgctaaggca aacggtcttc ctggaaagtt gatgcctgct cctttgctgt ctctcccaga
     4441 agacaaaatc ttagagacct tcaggcttag caacaaactg aaaggctcga cggatgaaat
     4501 gagtgcacct ggagcagaga ggggccctcc cgaggaccgc gacgccgagc ctcagcctgg
     4561 cagccccgcc gccgaatccc tggaggagcc agatgcggcc gccggcctca gcagcaccaa
     4621 gaaggctccg ccgccccgcg atcccggcac ccccgctacc aaagaaggcg cctgggaggc
     4681 catggccgtc gcccccgagg agcctccgcc cagcgccggc gaggacatcg tgggggacac
     4741 agcaccccct gacctgtgtg atcccgggag cttaacaggc gatgcgagcc cgttgtccca
     4801 ggacgcaaag gggatgatcg cggagggccc gcgggactcg gcgttggcgg aagccccgga
     4861 agggttgagt ccggctccgc ctgcgcggtc ggaggagccc tgcgagcagc cactccttgt
     4921 tcaccccagc ggggaccacg cccgggacgc tcaggaccca tcccagagct tgggcgcacc
     4981 cgaggccgca gagcggccgc cagctcctgt gctggacatg gccccggccg gtcacccgga
     5041 aggggacgct gagcctagcc ccggcgagag ggtcgaggac gccgcggcgc cgaaagcccc
     5101 aggcccttcc ccagcgaagg agaaaatcgg cagcctcaga aaggtggacc gaggccacta
     5161 ccgcagccgg agagagcgct cgtccagcgg ggagcccgcc agagagagca ggagcaagac
     5221 tgagggccac cgtcaccggc ggcgccgcac ctgcccccgg gagcgcgacc gccaggaccg
     5281 ccacgccccg gagcaccacc ccggccacgg cgacaggctc agccctggcg agcgccgctc
     5341 tctgggcagg tgcagtcacc accactcccg acaccggagc ggggtggagc tggactgggt
     5401 cagacaccac tacaccgagg gcgagcgtgg ctggggccgg gagaagttct accccgacag
     5461 gccgcgctgg gacaggtgcc ggtactacca tgacaggtac gccctgtacg ctgcccggga
     5521 ctggaagccc ttccacggcg gccgcgagca cgagcgggcc gggctgcacg agcggccgca
     5581 caaggaccac aaccggggcc gtaggggctg cgagccggcc cgggagaggg agcggcaccg
     5641 ccccagcagc ccccgcgcag gcgcgcccca cgccctcgcc ccgcaccccg accgcttctc
     5701 ccacgacaga actgcacttg tagccggaga caactgtaac ctctctgatc ggtttcacga
     5761 acacgaaaat ggaaagtccc ggaaacggag acacgacagt gtggagaaca gtgacagtca
     5821 tgttgaaaag aaagcccgga ggagcgaaca gaaggatcct ctagaagagc ctaaagcaaa
     5881 gaagcacaaa aaatcaaaga agaaaaagaa atccaaagac aaacaccgag accgcgactc
     5941 caggcatcag caggactcag acctctcagc agcgtgctct gacgctgacc tccacagaca
     6001 caaaaaaaag aagaagaaaa agaagagaca ttcaagaaaa tcagaggact ttgttaaaga
     6061 ttcagaactg cacttaccca gggtcaccag cttggagact gtcgcccagt tccggagagc
     6121 ccagggtggc tttcctctct ctggtggccc gcctctggaa ggcgtcggac ctttccgtga
     6181 gaaaacgaaa cacttacgga tggaaagcag ggatgacagg tgtcgtctct ttgagtatgg
     6241 ccagggtaag aggagatact tggaattagg aagaggatcc aaagaaaccg ctgctgctaa
     6301 attcgaacgc cagcacatgg acagcggagc cggcgcagga gccggcgctg acgcgcctga
     6361 ctacaaagac gatgacgaca agggagatta caaggatgac gatgacaaag gcgcgtcgat
     6421 ggacgagaag accaccggct ggcggggcgg ccacgtggtg gagggcctgg ccggcgagct
     6481 ggagcagctg cgggccaggc tggagcacca ccctcagggc cagcgggagc cctagcggcc
     6541 gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     6601 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
     6661 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     6721 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     6781 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg
     6841 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg
     6901 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc
     6961 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc
     7021 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc
     7081 ggcgcctacg ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat
     7141 cgagtgccgc atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga
     7201 gggcaccccc aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac
     7261 cttcagcccc tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta
     7321 ccccagcggc tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac
     7381 ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga
     7441 ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt
     7501 gatcttcacc gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg
     7561 cgataacgtg ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta
     7621 cagcttcgtg gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca
     7681 gaacgggggc cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct
     7741 gggcatcgtg gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc
     7801 tcagtcgtcc aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga
     7861 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga
     7921 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     7981 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     8041 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     8101 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     8161 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     8221 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     8281 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     8341 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     8401 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     8461 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc
     8521 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     8581 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     8641 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     8701 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     8761 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     8821 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     8881 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     8941 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     9001 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tccgccttcg
     9061 ccctcagacg agtcggatct ccctttggcc gcctccccgc ctggtacctt taagaccaat
     9121 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     9181 gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt
     9241 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     9301 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     9361 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     9421 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     9481 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     9541 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9601 tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc
     9661 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9721 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat
     9781 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     9841 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     9901 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     9961 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
    10021 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    10081 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    10141 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    10201 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    10261 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    10321 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    10381 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    10441 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    10501 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    10561 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    10621 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    10681 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    10741 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    10801 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    10861 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    10921 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    10981 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    11041 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    11101 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    11161 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    11221 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    11281 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    11341 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    11401 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    11461 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    11521 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    11581 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    11641 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    11701 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    11761 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    11821 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    11881 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    11941 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
    12001 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
    12061 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
    12121 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
    12181 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
    12241 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
    12301 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
    12361 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
