LOCUS       V043386                11659 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043386
VERSION     V043386
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5685
                     /label="USP43(NM_153210)"
                     /note="USP43(NM_153210)"
                     /gene="USP43"
     CDS             5692..5757
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5801..6012
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6025..6293
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6322..6331
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6352..7014
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7084..7137
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7138..7737
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7738..8326
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8400..8633
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8705..8826
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8866..9001
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9034..9050)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9058..9074
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9082..9112)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9127..9148
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9436..10024)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10195..11055)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11056..11160)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11634..11650
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg acctgggccc cggggacgcg
     2341 gcaggagggg gaccgctcgc gccccggccc cgccgccgcc gctccctgcg ccgcctgttc
     2401 agccgcttcc tgctggcgct gggcagccgc tcacgccccg gggactcacc gccccggccc
     2461 cagccgggac actgtgatgg cgacggtgag gggggcttcg cctgcgcccc gggcccagtt
     2521 ccagcggccc ccgggagccc cggggaggaa cgcccgcccg gaccccagcc ccagctccag
     2581 ctccccgccg gcgatggggc gcggccgccg ggcgctcagg gcttgaagaa ccacggcaac
     2641 acctgtttca tgaacgcggt ggtgcagtgt ctcagcaaca ccgacctgct ggccgagttc
     2701 ctggcgctgg ggcgctaccg ggcggctccg ggccgcgccg aggtcaccga gcagctggcg
     2761 gcgctggtgc gcgcgctctg gactcgcgaa tacacgcccc aactttccgc ggagttcaag
     2821 aatgcagttt ccaagtacgg ctctcagttc caaggcaatt cccagcacga cgccctggaa
     2881 ttcctgctct ggttgctgga tcgtgtacat gaggacctgg agggttcatc ccgagggccg
     2941 gtgtcggaga agcttccgcc tgaagccact aaaacctctg agaactgcct gtcaccatca
     3001 gctcagcttc ctctaggtca aagctttgtg caaagccact ttcaagcaca atatagatct
     3061 tccttgactt gtccccactg cctgaaacag agcaacacct ttgatccttt cctgtgtgtg
     3121 tccctaccta tccccttgcg ccagacgagg ttcttgagtg tcaccttggt cttcccctct
     3181 aagagccagc ggttcctgcg ggttggcctg gccgtgccga tcctcagcac agtggcagcc
     3241 ctgaggaaga tggttgcaga ggaaggaggc gtccctgcag atgaggtgat cttggttgaa
     3301 ctgtatccca gtggattcca gcggtctttc tttgatgaag aggacctgaa taccatcgca
     3361 gagggagata atgtgtatgc ctttcaagtt cctccctcac ccagccaggg gactctctca
     3421 gctcatccac tgggtctgtc ggcctcccca cgcctggcag cccgtgaggg ccagcgattc
     3481 tccctctctc tccacagtga gagcaaggtg ctaatcctct tctgtaactt ggtggggtca
     3541 gggcagcagg ctagcaggtt tgggccaccc ttcctgataa gggaagacag agctgtttcc
     3601 tgggcccagc tccagcagtc tatcctcagc aaggtccgcc atcttatgaa gagtgaggcc
     3661 cctgtacaga acctggggtc tctgttctcc atccgtgttg tgggactctc tgtggcctgc
     3721 agctatttgt ctccgaagga cagtcggccc ctctgtcact gggcagttga cagggttttg
     3781 catctcagga ggccaggagg ccctccacat gtcaagctgg cggtggagtg ggatagctct
     3841 gtcaaggagc gcctgttcgg gagcctccag gaggagcgag cgcaggatgc cgacagtgtg
     3901 tggcagcagc agcaggcgca tcagcagcac agctgtacct tggatgaatg ttttcagttc
     3961 tacaccaagg aggagcagct ggcccaggat gacgcctgga agtgtcctca ctgccaagtc
     4021 ctgcagcagg ggatggtgaa gctgagtttg tggacgctgc ctgacatcct catcatccac
     4081 ctcaaaaggt tctgccaggt gggcgagaga agaaacaagc tctccacgct ggtgaagttt
     4141 ccgctctctg gactcaacat ggctccccat gtggcccaga gaagcaccag ccctgaggca
     4201 ggactgggcc cctggccttc ctggaagcag ccggactgcc tgcccaccag ttacccgctg
     4261 gacttcctgt acgacctgta tgccgtctgc aaccaccatg gcaacctgca aggtgggcat
     4321 tacacagcct actgccggaa ctctctggat ggccagtggt acagttatga tgacagcacg
     4381 gtggaaccgc ttcgagaaga tgaggtcaac accagagggg cttatatcct gttctatcag
     4441 aagcggaaca gcatccctcc ctggtcagcc agcagctcca tgagaggctc taccagctcc
     4501 tccctgtctg atcactggct cttacggctc gggagccacg ctggcagcac aaggggaagc
     4561 ctgctgtcct ggagctctgc cccctgcccc tccctgcccc aggttcctga ctctcccatc
     4621 ttcaccaaca gcctctgcaa tcaggaaaag ggagggttgg agcccaggcg tttggtacgg
     4681 ggcgtgaaag gcagaagcat tagcatgaag gcacccacca cttcccgagc caagcaggga
     4741 ccattcaaga ccatgcctct gcggtggtcc tttggatcca aggagaaacc accaggtgcc
     4801 tccgtcgagt tggtggagta cttggaatcc agacgaagac ctcggtccac gagccagtcc
     4861 attgtgtcgc tgttgacggg cactgcgggt gaggatgaga agtcagcatc gccgaggtcc
     4921 aacgtcgccc ttcctgctaa cagcgaagat ggtgggcggg ccattgaaag aggtccagcc
     4981 ggggtgccct gtccctcggc tcaacccaac cactgtctgg cccctggaaa ctcagatggt
     5041 ccaaacacag caaggaaact caaggaaaat gcagggcagg acatcaagct tcccagaaag
     5101 tttgacctgc ctctcactgt gatgccttca gtggagcatg agaaaccagc tcgaccggag
     5161 ggccagaagg ccatgaactg gaaggagagc ttccagatgg gaagcaaaag cagcccaccc
     5221 tccccctata tgggattctc tggaaacagc aaagacagtc gccgaggcac ctctgagcta
     5281 gacagacccc tgcaggggac actcaccctt ctgaggtccg tgtttcggaa gaaggagaac
     5341 aggaggaatg agagggcaga ggtctctcca caggtgcccc ccgtctccct ggtgagtggc
     5401 gggctgagcc ctgccatgga cgggcaggct ccaggctcac ctcctgccct caggatccca
     5461 gagggcctgg ccaggggcct gggcagccgg ctcgagaggg atgtctggtc agcccccagc
     5521 tctctccgcc tccctcgtaa agccagcagg gccccgagag gcagtgcact gggcatgtca
     5581 caaaggactg ttccagggga gcaggcttct tatggcacct ttcagagagt caaatatcac
     5641 actctttctt taggtcgaaa gaaaacctta ccggagtcca gctttggatc cgactacaaa
     5701 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtag
     5761 cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     5821 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     5881 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     5941 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     6001 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     6061 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     6121 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     6181 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     6241 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     6301 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg
     6361 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc
     6421 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc
     6481 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc
     6541 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc
     6601 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc
     6661 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac
     6721 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc
     6781 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc
     6841 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc
     6901 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc
     6961 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc
     7021 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct
     7081 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg
     7141 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     7201 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     7261 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     7321 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     7381 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     7441 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     7501 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     7561 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     7621 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     7681 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat
     7741 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     7801 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     7861 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     7921 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     7981 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     8041 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     8101 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     8161 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     8221 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     8281 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag
     8341 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     8401 ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta ctgggtctct
     8461 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8521 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8581 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     8641 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     8701 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8761 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8821 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     8881 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8941 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9001 ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
     9061 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     9121 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     9181 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     9241 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     9301 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     9361 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9421 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9481 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9541 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9601 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9661 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9721 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9781 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9841 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     9901 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9961 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    10021 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    10081 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    10141 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    10201 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    10261 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    10321 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    10381 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10441 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10501 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10561 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10621 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10681 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10741 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10801 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10861 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10921 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10981 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    11041 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    11101 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    11161 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    11221 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    11281 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    11341 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    11401 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    11461 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    11521 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    11581 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    11641 gacggccagt gccaagctg
//
